We narrowed to 13,681 results for: sequence
-
Plasmid#104309Purposecodes for a fusion of fluorescent protein mGarnet and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mitoDepositorInsertmito-mGarnet
ExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-polg guide
Plasmid#107598PurposeAn entry vector with U6a promoter driving polg guide RNA expressionDepositorInsertpolg guide RNA sequence
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag-NLS-Smad2
Plasmid#14931DepositorInsertNLS-Smad2 (SMAD2 Human)
TagsFlagExpressionMammalianMutationfull-length Smad2 is fused to a nuclear-localizat…Available SinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-WNT3A
Plasmid#108345Purposesynchronize trafficking of WNT3A from the ER (RUSH System)DepositorInsertWNT3A, Wnt family member 3A (WNT3A Human)
TagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianPromoterCMVAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b T7pCONS sfGFP
Plasmid#154467PurposepET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherryGGBsMTS
Plasmid#133625PurposeExpression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherryGGBsMTS
ExpressionBacterialAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPLP4HS-mCf
Plasmid#229988PurposeLIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.DepositorTypeEmpty backboneTagsHis6 and Twin-Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP2S-mCf
Plasmid#229986PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.DepositorTypeEmpty backboneTagsStrep-tag IIExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
env8 reverse J1/13
Plasmid#99831PurposeGFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.DepositorInsertenv8 reverse J1/13
TagsGFPuvExpressionBacterialMutationJ1/13 sequence is reversedAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCoofy64
Plasmid#122016PurposeBaculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionInsectAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBFv-UAS3
Plasmid#138399Purpose10xUAS expression vector with a vermilion marker and a phiC31 attB sequenceDepositorTypeEmpty backboneExpressionInsectPromoterUASAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIPlac204TKC-E2-Crimson-HDEL
Plasmid#38771DepositorInsertE2-Crimson
TagsLinker peptide and yeast ER retention signal sequ…ExpressionYeastPromoterTPI1Available SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-Venus
Plasmid#87613PurposeActin-targeted Venus (YFP).DepositorInsertLifeAct-Venus
Tags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianPromoterCMVAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCD-Ugi22
Plasmid#207004PurposepGGC000 Greengate entry vector containing Utricularia gibba insulator 22 (Ugi22) sequence (ID2547)DepositorInsertUtricularia gibba insulator 22
UseGolden gate compatible cloning vectorAvailable SinceNov. 8, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCD-Ugi21
Plasmid#207005PurposepGGC000 Greengate entry vector containing Utricularia gibba insulator 21 (Ugi21) sequence (ID9063)DepositorInsertUtricularia gibba insulator 21
UseGolden gate compatible cloning vectorAvailable SinceNov. 8, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-ALFA-tag-Gal4-VP16
Plasmid#199465PurposeExpression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.DepositorInsertGal4-ALFA-tag-VP16
ExpressionMammalianAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
LacI INCENP
Plasmid#45236PurposeTarget INCENP to lac operator sequenceDepositorInsertLacI, INCENP-Δcen, mCherry (lacI )
TagsLacI (lac repressor protein) and mCherryExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)Promotert7Available SinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3XFLAG-EGFP-NLS hygro
Plasmid#87781PurposeInducible expression of EGFP with a C-terminal NLS sequenceDepositorInserteGFP
UseLentiviralTags3xFLAG and NLSExpressionMammalianAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICSL70008
Plasmid#50336DepositorInsertpFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
UsePlant expressionMutationBsaI/BbsI sites removed by point-mutationAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
RNF168-Halo HRD
Plasmid#207080PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
ExpressionMammalianPromoterNoneAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2 HRD
Plasmid#207544PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cellsDepositorInsertHaloTag flanked by human WIPI2 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Dronpa-Kras
Plasmid#182329PurposePlasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.DepositorInsertDronpa-Kras
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-lynD3cpV
Plasmid#37472DepositorInsertD3cpv second generation cameleon (calcium sensor)
Tagsthe myristoylation and palmitoyla- tion sequence …ExpressionMammalianPromoterCMVAvailable SinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMGB
Plasmid#139932PurposeM. magneticum/E. coli shuttle vector (partial sequence of pMGT and p15A)DepositorInsertsfGFP
ExpressionBacterialPromoterPJ23101Available SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB19-PapRS#34-OmpA
Plasmid#231105PurposeVector for bacterial expression of a protein carrying the non-canonical amino acid p-(phenylazo)-L-phenylalanine (encoding the OmpA signal sequence for periplasmic secretion)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-83:EZH2-mEGFP
Plasmid#164499PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human EZH2DepositorInsertEZH2 Homology Arms with mEGFP-linker (EZH2 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceFeb. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pgRNA-non-target
Plasmid#129465PurposeDerived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.DepositorInsertNon-target/random gRNA
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV HDM2(NES-)
Plasmid#12087DepositorInsertHDM2 (MDM2 Human)
ExpressionMammalianMutationNuclear-export sequence mutant (NES-): L205A, I20…Available SinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only