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We narrowed to 18,759 results for: nls

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Showing: 9361 - 9400 of 18759 results

  1. Cer(1-158)-gamma-5 in pcDNAI/Amp

    Plasmid
    #55625
    Purpose
    An amino-terminal mCerulean fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cer(1-158)-gamma-5 (GNG5 Human, Aequorea victoria)
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-5 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55625-plasmid-map-sequence-id-115180

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  2. CFP(159-238)-beta-5 in pcDNAI/Amp

    Plasmid
    #55763
    Purpose
    A carboxyl-terminal CFP fragment was fused to Gbeta-5. When co-expressed with an amino terminal CFP or YFP fragment fused to a Ggamma subunit or RGS7, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CFP(159-238)-beta-5 (GNB5 Human, Aequorea victoria)
    Tags
    CFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    CFP(159-238) includes a substitution of His for A…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55763-plasmid-map-sequence-id-114867

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  3. YFP(1-158)-beta-2 in pcDNAI/Amp

    Plasmid
    #54469
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP(1-158)/beta-2 (GNB2 Human, Aequorea victoria)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54469-plasmid-map-sequence-id-114883

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  5. pMuLE ENTR SV40-hCas9 L3-L2

    Plasmid
    #62133
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized Cas9
    Use
    CRISPR and Gateway Cloning
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62133-plasmid-map-sequence-id-101417

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  14. pTALEN(Nd154C63) MND

    Plasmid
    #50432
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    MND
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50432-plasmid-map-sequence-id-75353

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  18. pCAG-attBr-Cre-rc-attP

    Plasmid
    #51264
    Purpose
    Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation
    Depositor
    Pawel Pelczar
    Publication
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    attBr-Cre-rc-attP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    51264-plasmid-map-sequence-id-77874

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  22. pcDXC3GMS

    Plasmid
    #49031
    Purpose
    FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide
    Depositor
    Raimund Dutzler, Eric Geertsma
    Publication
    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.
    Type
    Empty backbone
    Tags
    3C protease site (PreScission site), EGFP, MYC, a…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49031-plasmid-map-sequence-id-71989

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  25. pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49228
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Publication
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49228-plasmid-map-sequence-id-73645

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  26. pGL1.7XX

    Plasmid
    #49344
    Purpose
    luciferase reporter with mouse Bmp2 promoter
    Depositor
    Melissa Rogers
    Publication
    Abrams et al J Biol Chem. 2004 Apr 16;279(16):15916-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bmp2 distal promoter (Bmp2 Mouse)
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    nt –1237 to +471
    Promoter
    Bmp2 distal promoter
    Available since
    Dec. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    49344-plasmid-map-sequence-id-73264

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  27. pAC148-pCR8-dCas9VP96

    Plasmid
    #48220
    Purpose
    dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP96 activation domain
    Use
    CRISPR and Gateway Cloning
    Tags
    HA Tag and VP96
    Mutation
    D10A;H840A
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48220-plasmid-map-sequence-id-69505

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  35. pEMS1611

    Plasmid
    #29276
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple139 (NR2E1 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    29276-plasmid-map-sequence-id-13691

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  36. pEMS1612

    Plasmid
    #29277
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple140 (NR2E1 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    29277-plasmid-map-sequence-id-13692

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  38. pEMS1418

    Plasmid
    #29214
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple112 (HCRT Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Nov. 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29214-plasmid-map-sequence-id-13629

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  39. pEMS1401

    Plasmid
    #29198
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter
    Depositor
    Elizabeth Simpson
    Publication
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Nov. 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    29198-plasmid-map-sequence-id-13613

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Showing: 9361 - 9400 of 18759 results