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Plasmid#235473PurposeMessage phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030)DepositorArticleInsertsgRNA3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK001.23
Plasmid#235484Purposedual NOT gate receiver with bs for sgRNA2 and sgRNA3DepositorArticleInsertNOT gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK002.1
Plasmid#235460PurposeMessage phagemid carrying sgRNA1 (prom. J23119, backbone pBR322)DepositorArticleInsertsgRNA1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK002.2
Plasmid#235461PurposeMessage phagemid carrying sgRNA2 (prom. J23119, backbone pBR322)DepositorArticleInsertsgRNA2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK002.3
Plasmid#235462PurposeMessage phagemid carrying sgRNA3 (prom. J23119, backbone pBR322)DepositorArticleInsertsgRNA3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK002.4
Plasmid#235463PurposeMessage phagemid carrying sgRNA4 (prom. J23119, backbone pBR322)DepositorArticleInsertsgRNA4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK002.5
Plasmid#235464PurposeMessage phagemid carrying sgRNA5 (prom. J23119, backbone pBR322)DepositorArticleInsertsgRNA5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK002.6
Plasmid#235465PurposeMessage phagemid carrying sgRNA6 (prom. J23119, backbone pBR322)DepositorArticleInsertsgRNA6
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK302.1
Plasmid#235466PurposeMessage phagemid carrying sgRNA1 (prom. J23110, backbone pBR322)DepositorArticleInsertsgRNA1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK302.2
Plasmid#235467PurposeMessage phagemid carrying sgRNA2 (prom. J23110, backbone pBR322)DepositorArticleInsertsgRNA2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK302.3
Plasmid#235468PurposeMessage phagemid carrying sgRNA3 (prom. J23110, backbone pBR322)DepositorArticleInsertsgRNA3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK302.4
Plasmid#235469PurposeMessage phagemid carrying sgRNA4 (prom. J23110, backbone pBR322)DepositorArticleInsertsgRNA4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK302.5
Plasmid#235470PurposeMessage phagemid carrying sgRNA5 (prom. J23110, backbone pBR322)DepositorArticleInsertsgRNA5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK001.1
Plasmid#235449PurposeNOT gate receiver with binding site (bs) for sgRNA1DepositorArticleInsertNOT gate
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHPHS309
Plasmid#228230PurposeGFP dropout backbone for sgRNA library cloningDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJEC619
Plasmid#182955PurposeSYNZIP18-Cascade.DepositorInsertSynZip18-Cascade
UseTagsSynZip18ExpressionBacterialMutationSynZip18 fusion to CasAPromoterJ23150Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-Un1Cas12f1
Plasmid#205143PurposeUn1Cas12f1 Gateway entry plasmidDepositorInsertUn1Cas12f1
UseCRISPRTagsNLSExpressionMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T2A-H2B mCitirine
Plasmid#210471PurposeCoexpression of Cas9 and H2B mCitrineDepositorInsertCas9-T2A-H2B mCitirine
UseCRISPR and Synthetic BiologyTagsmCitrine on C terminal of H2BExpressionMammalianMutationCas9 contains 3xFLAG tag and nuclear localization…PromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector
Plasmid#194726PurposeCAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vectorDepositorArticleInsertAsCpf1(D908A)
UseCRISPRTagsExpressionMammalianMutationD908APromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBoL1P4GA
Plasmid#112910PurposeExpress a cloned sgRNA from AtU626 promoterDepositorInsertAtU626 PROMOTER-Laz-sgRNA
UseTagsExpressionMutationPromoterAtU626Available sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH48
Plasmid#125813PurposeLevel0 Csy4DepositorInsertCsy4
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZNF57-donor
Plasmid#112296PurposeCRISPR donor plasmid to tag human transcription factor ZNF57 with GFPDepositorInsertZNF57 homology arms flanking EGFP-IRES-Neo cassette (ZNF57 Human)
UseCRISPRTagsExpressionMutationrs2288959, rs2288958, rs10410539, hg38:19:2918211…PromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF215-donor
Plasmid#113804PurposeCRISPR donor plasmid to tag human transcription factor ZNF215 with GFPDepositorInsertZNF215 homology arms flanking EGFP-IRES-Neo cassette (ZNF215 Human)
UseCRISPRTagsExpressionMutationrs2239733, rs2239732, hg38:11:6956348:T>G, rs1…PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNR2F1-donor
Plasmid#112388PurposeCRISPR donor plasmid to tag human transcription factor NR2F1 with GFPDepositorInsertNR2F1 homology arms flanking EGFP-IRES-Neo cassette (NR2F1 Human)
UseCRISPRTagsExpressionMutationrs974916569PromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF211-donor
Plasmid#112382PurposeCRISPR donor plasmid to tag human transcription factor ZNF211 with GFPDepositorInsertZNF211 homology arms flanking EGFP-IRES-Neo cassette (ZNF211 Human)
UseCRISPRTagsExpressionMutationhg38:19:57641976:T>C, hg38:19:57641979:A>T,…PromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF766-donor
Plasmid#112352PurposeCRISPR donor plasmid to tag human transcription factor ZNF766 with GFPDepositorInsertZNF766 homology arms flanking EGFP-IRES-Neo cassette (ZNF766 Human)
UseCRISPRTagsExpressionMutationhg38:19:52290357:G>T, hg38:19:52290373:A>G,…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF84-donor
Plasmid#112330PurposeCRISPR donor plasmid to tag human transcription factor ZNF84 with GFPDepositorInsertZNF84 homology arms flanking EGFP-IRES-Neo cassette (ZNF84 Human)
UseCRISPRTagsExpressionMutationrs623100, hg38:12:133058872:T>C, hg38:12:13305…PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTBPL1-donor
Plasmid#112316PurposeCRISPR donor plasmid to tag human transcription factor TBPL1 with GFPDepositorInsertTBPL1 homology arms flanking EGFP-IRES-Neo cassette (TBPL1 Human)
UseCRISPRTagsExpressionMutationrs35776966, hg38:6:133987646:GTGTAT>-PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMAD3-donor
Plasmid#112311PurposeCRISPR donor plasmid to tag human transcription factor SMAD3 with GFPDepositorInsertSMAD3 homology arms flanking EGFP-IRES-Neo cassette (SMAD3 Human)
UseCRISPRTagsExpressionMutationhg38:15:67189762:C>T, rs11629568, rs8025774PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJH375
Plasmid#86841Purposemamalian expression of anti-CRISPR protein AcrIIA3DepositorInsertacrIIA3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6a:sgRNA#1
Plasmid#64245Purposeexpresses sgRNA under U6a promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6b:sgRNA#3
Plasmid#64247Purposeexpresses sgRNA under U6b promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6c:sgRNA#4
Plasmid#64248Purposeexpresses sgRNA under U6c promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6a:sgRNA#2
Plasmid#64246Purposeexpresses sgRNA under U6a promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6d:sgRNA#5
Plasmid#64249Purposeexpresses sgRNA under U6d promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDonor_ShCAST_kanR
Plasmid#127924PurposeKanamycin resistance donor for ShCASTDepositorInsertKanR, R6K origin of replication
UseTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-8x-gRNA-eGFP
Plasmid#60718PurposeeGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activationDepositorInsert8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pScCas9
Plasmid#117700PurposeMammalian expression vector encoding WT ScCas9DepositorInsertStreptococcus canis Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human ICAM-2
Plasmid#8634DepositorInsertICAM-2 (ICAM2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-tdTomato-VPR
Plasmid#232632PurposeVPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacerDepositorInsertdCas9-tdTomato-VPR
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGGDestTol2LC-5sgRNA
Plasmid#64243Purposedestination vector for five U6x:sgRNA cassettesDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
U6_crRNA_mCherry
Plasmid#197609PurposeExpresses a Cas7-11 crRNA and mCherryDepositorInsertU6_crRNA_mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGGDestTol2LC-4sgRNA
Plasmid#64242Purposedestination vector for four U6x:sgRNA cassettesDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1-BFP
Plasmid#120577PurposeLentiviral plasmid to co-express a guide RNA and EBFP.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGDestTol2LC-2sgRNA
Plasmid#64240Purposedestination vector for two U6x:sgRNA cassettesDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGDestTol2LC-3sgRNA
Plasmid#64241Purposedestination vector for three U6x:sgRNA cassettesDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGDestTol2LC-1sgRNA
Plasmid#64239Purposedestination vector for one U6x:sgRNA cassetteDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXT130_TwinStrep-SUMO-ShTnsC
Plasmid#135526PurposeProtein expression construct for ShTnsCDepositorInsertShTnsC
UseTagsTwinStrep-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
DECOR
Plasmid#219545PurposeExpression of CMV-TadA8e-NLS-dCas13d-NLS in mammalian cells (DECOR expression)DepositorInsertDECOR
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only