We narrowed to 42,069 results for: LAS

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pACAGW-oChiEFtdTomato-AAV

Plasmid

#32846

Purpose

AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

Depositor

Rory McQuiston

Article

Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

Insert

oChiEF

Use

AAV

Tags

tdTomato

Promoter

CAGG

Available Since

March 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCAT-YFP-TetR

Plasmid

#59018

Purpose

Expresses a fusion of yellow ﬂuorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii

Depositor

David Sibley

Article

Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

Insert

YFP-TetR

Use

Toxoplasma expression

Promoter

alpha-tubulin

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-BLST-dCas9-VPR

Plasmid

#115148

Purpose

Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

BlastR-P2A-dCas9-VPR

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51-GOLPH3

Plasmid

#21690

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Invitrogen gateway destination vector

Tags

6xHIS and V5

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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p0857 pBR322 SV40

Plasmid

#10877

Depositor

Peter Howley

Insert

SV40

Expression

Bacterial

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pJRH-1346 U6-B2M sgRNA Gag-pol v2

Plasmid

#201917

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Expression

Mammalian

Promoter

CAG and Human U6

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta13

Plasmid

#13559

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A Delta13

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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TLCV2-LoxP

Plasmid

#127098

Purpose

LentiCRISPR v2 was modified into an all-in-one dox inducible system.

Depositor

Adam Karpf

Insert

Cas9-2A-eGFP

Use

Lentiviral

Promoter

U6

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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LHP1481 - TOPO-ProteinA

Plasmid

#32225

Depositor

Linda Hicke

Insert

Protein A

Use

Cloning

Promoter

P-lac

Available Since

Oct. 24, 2011

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat FL

Plasmid

#32449

Depositor

Anthony Means

Article

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S3L, G96R, and P108L

Promoter

SV40

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only
GFP-TRIM66

Plasmid

#65400

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM66 (TRIM66 Human)

Tags

GFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pGEM2 hTSP-1

Plasmid

#12993

Depositor

Jack Lawler

Insert

TSP-1 (THBS1 Human)

Expression

Bacterial

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

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pSIRIP-puro shRNA p19-1

Plasmid

#14089

Depositor

Tyler Jacks

Article

Sage et al Nature. 2003 Jul 10. 424(6945):223-8.

Insert

p19 ARF shRNA - 1 (Cdkn2a Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-lox-GFP shRNA p19-2

Plasmid

#14091

Depositor

Tyler Jacks

Article

Sage et al Nature. 2003 Jul 10. 424(6945):223-8.

Insert

p19 ARF shRNA -2 (Cdkn2a Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-ms-Klf5

Plasmid

#50787

Purpose

retroviral expression of mouse Klf5

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Klf5 (Klf5 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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TagRFP-T_A1aY1

Plasmid

#158751

Purpose

Lentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

TagRFP-T_A1aY1

Use

Lentiviral

Tags

TagRFP-T

Expression

Mammalian

Promoter

CAG (CMV enhancer and chicken beta actin promoter)

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-OMP25

Plasmid

#141150

Purpose

Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat)

Tags

AcGFP

Expression

Mammalian

Mutation

OMP25 c terminus from addgene plasmid #69598

Promoter

CMV

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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mATF4

Plasmid

#24874

Depositor

Fumihiko Urano

Article

Ghosh et al PLoS One. 2010 . 5(3):e9575.

Insert

mouse ATF4 (Atf4 Mouse)

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available Since

May 17, 2010

Availability

Academic Institutions and Nonprofits only
pEZYshuttle

Plasmid

#18703

Purpose

pShuttle-CMV converted Gateway Destination vector for AdEasy adenoviral vector system

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pDCC5

Plasmid

#59984

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Insert

Cas9-D10A

Use

CRISPR

Tags

HA

Expression

Insect

Mutation

D10A

Promoter

hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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