We narrowed to 150,160 results for: ins
-
Plasmid#113308Purposehuman Mre11 in baculovirus transfer vectorDepositorAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only
-
mKO2-2A-mTurquoise2
Plasmid#98838PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmKO2-2A-mTurquoise2
ExpressionMammalianPromoterCMVAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORE303N
Plasmid#194438PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, KpnI site for gRNA insertionDepositorInsertCas9
UseCRISPRExpressionPlantPromoter2x35SAvailable SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hPMS2
Plasmid#129425PurposeExpression human PMS2 protein using baculovirusDepositorAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-sfGFPx2
Plasmid#51559PurposeSuperfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)DepositorInsertsuperfolder GFP
TagsGLESTCRHASLAVLADERRFSA and MARARAExpressionBacterialMutationContains additonal aa in the N and C terminus (in…Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMLH1
Plasmid#129426PurposeExpression human MLH1 protein using baculovirusDepositorAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDV05.1_pEF1a-iRFP720_frameshifted
Plasmid#200408PurposeExpresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent proteinDepositorInsertiRFP720 (frameshift mutant)
UseSynthetic BiologyExpressionMammalianAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMON-HsNMT1
Plasmid#67475PurposeExpression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteriaDepositorInsertHsNMT1 (NMT1 Human)
ExpressionBacterialAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-116:PCNA-mEGFP
Plasmid#170818PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human PCNADepositorInsertPCNA Homology Arms with mEGFP-linker (PCNA Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceJune 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBLADE_ONLY_A
Plasmid#168051PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
ExpressionBacterialPromoterJ23101**Available SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES
Plasmid#200406PurposeExpresses a compact, engineered version of ADAR2 constitutively in mammalian cellsDepositorInsertMCP-ADAR2dd(E488Q)-NES
UseSynthetic BiologyExpressionMammalianMutationE488Q (hyperactive)PromoterCMVAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
nos-Cas9.874Z1
Plasmid#112685PurposeExpress hSpCas9 under nanos promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoternanosAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymTq2-URA3
Plasmid#168057PurposeReplaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymTurquoise2
TagsymTurquoise2ExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-079:DCP1A-mEGFP
Plasmid#193922PurposeHomology arms and mEGFP-SRA for N-terminus tagging of human DCP1ADepositorInsertDCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
UseCRISPR; Donor templateTagsmEGFP-linkerMutationhomology arms contain point mutations to disrupt …Available SinceDec. 22, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-166:CDH1-mEGFP
Plasmid#193921PurposeHomology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1DepositorInsertcadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
UseAAV and CRISPR; Donor templateTagslinker-mEGFPMutationhomology arms contain point mutations to disrupt …Available SinceDec. 22, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]
Plasmid#71805PurposeGenerating transgenic flies for 4-hydroxytamoxifen-inducible DamIDDepositorInsertFL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]
ExpressionInsectAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKG I alpha WT
Plasmid#16390DepositorAvailable SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-DeltaJunD
Plasmid#68547PurposeAAV expression of DeltaJunDDepositorInsertDeltaJunD-IRES-GFP
UseAAVExpressionMammalianMutationtruncated splice variantPromoterCMVAvailable SinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT005-HaloGPM
Plasmid#163385Purpose2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN systemDepositorInsertHalotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)
UseLentiviralTagsmCherryExpressionBacterial and MammalianPromoterEF1aAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRXG
Plasmid#113643PurposeThis construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.DepositorInsertmRFP1-linker-sfGFP
UseSynthetic BiologyAvailable SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only