We narrowed to 9,202 results for: sacs

1. pEasyG1_hph

   Plasmid

   #184912

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs_FLAG-NDT1

   Plasmid

   #133254

   Purpose

   Express FLAG-tagged NDT1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   NDT1 (YIA6 Budding Yeast)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in human cells

   Promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpeCas9-HF

   Plasmid

   #192141

   Purpose

   Expresses highly specific SpeCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

   Insert

   SpeCas9-HF
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   SpeCas9-HF (R247A, N415A, S421A)

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGAN201

   Plasmid

   #129204

   Purpose

   yTRAP sensor of [RNQ+], detects aggregation of Rnq1

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   RNQ1 (RNQ1 Budding Yeast, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SUP35

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p221k-erTq2CFP

   Plasmid

   #71264

   Purpose

   Entry clone containing ER-localized Turqoise2. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system.

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   Tq2CFP
   

   Use

   Gateway

   Tags

   ER retention signal HDEL and signal peptide (ER)…

   Expression

   Mutation

   Promoter

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDuet-1 TIM8,13

   Plasmid

   #170282

   Purpose

   Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

   Insert

   yeast Tim8 and Tim13 (TIM8 Budding Yeast)
   

   Use

   Tags

   TEV-cleavable N-terminal His tag on Tim13 (on bac…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMyc4ElbLuc.

   Plasmid

   #53246

   Purpose

   reporter plasmid containing 4 myc cDNA binding sites

   Depositor

   Roger Davis

   Article

   Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

   Insert

   pMyc4ElbLuc (MYC Human)
   

   Use

   Tags

   Expression

   Mutation

   To prepare plasmids with one, two, three, and fou…

   Promoter

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

8. YIp128-CUP1-NUbo-GFP-His6

   Plasmid

   #212790

   Purpose

   Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-GFP-His6
   

   Use

   Integrative vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET151-Vps4(101-437)-Hcp1

   Plasmid

   #87737

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100

   Promoter

   T7

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179051

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolus

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-RPC19-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pST1374-D10A-GCN4

    Plasmid

    #113025

    Purpose

    Expresses D10A-GCN4 in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    D10A-GCN4 (GCN4 Budding Yeast, S. pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFL-EGFP-3xFlag-Fml1

    Plasmid

    #193058

    Purpose

    Baculovirus expression of S.pombe Fml1 for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    Fml1 (fml1 Fission Yeast)
    

    Use

    Tags

    3xFlag

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIMVAd39T

    Plasmid

    #98303

    Purpose

    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEasyG1_nat

    Plasmid

    #184911

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAEL007097-Cas9

    Plasmid

    #100705

    Purpose

    The promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL007097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL007097 and Opie2

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGAN200

    Plasmid

    #129203

    Purpose

    yTRAP sensor of [PSI+], detects aggregation of Sup35

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Sup35NM (SUP35 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    N terminal and middle domains

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

    Plasmid

    #204288

    Purpose

    yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    pHis3

    Available since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. myc-POMP

    Plasmid

    #86764

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    human POMP (POMP Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS313_ATP3

    Plasmid

    #120253

    Purpose

    Expression of ATP3 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ATP3

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMyc3ElbLuc

    Plasmid

    #53245

    Purpose

    reporter plasmid containing 3 myc cDNA binding sites

    Depositor

    Roger Davis

    Article

    Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

    Insert

    pMyc3ElbLuc (MYC Human)
    

    Use

    Tags

    Expression

    Mutation

    To prepare plasmids with one, two, three, and fou…

    Promoter

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pBBK83 pHomL-RPL18Bp-ERG6-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179067

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Scarlet(rfp), a marker for the lipid droplet

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-ERG6-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBBK82 pHomL-RPL18Bp-Scarlet(rfp)-Link-ATG8-SSA1t-HomR (AmpR)

    Plasmid

    #179066

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-ATG8, a marker for the autophagosome

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-ATG8
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBBK70 pHomL-RPL18Bp-Scarlet(rfp)-Link-TUB1-SSA1t-HomR (AmpR)

    Plasmid

    #179054

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-TUB1, a marker for the microtubules

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-TUB1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG

    Plasmid

    #176400

    Purpose

    Expresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    FRB-RGG-GFP-RGG-RGG
    

    Use

    Tags

    FRB and GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBBK76 pHomL-RPL18Bp-PIL1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179060

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Scarlet(rfp), a marker for the eisosomes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-PIL1-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAEL003877-Cas9

    Plasmid

    #100581

    Purpose

    The promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL003877-hspcas9-GFP
    Opie-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL003877 and Opie2

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FLAG-MED8

    Plasmid

    #15406

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    MED8 (MED8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pAVA-LGF2-Gal11p

    Plasmid

    #127481

    Purpose

    Positive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    LGF2-Gal11p (GAL11 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. -227 hTF (mutant NF-kB)

    Plasmid

    #15443

    Depositor

    Nigel Mackman

    Article

    Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

    Insert

    Tissue factor promoter (F3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    NF-kappaB binding domain mutated

    Promoter

    Available since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pEasyG3_nat

    Plasmid

    #184918

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge