We narrowed to 22,191 results for: his

1. pCS2-wt-EGFP

   Plasmid

   #191117

   Purpose

   An wt EGFP control for Zebrafish

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   enhanced green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-pro-sumo2

   Plasmid

   #25102

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   pro-SUMO2 (SUMO2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

3. RP1B-PP1alpha

   Plasmid

   #51768

   Purpose

   Expresses PP1 alpha residues 7-330

   Depositor

   Wolfgang Peti

   Article

   Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

   Insert

   pp1 alpha
   

   Use

   Tags

   His, TEV protease site, and Thio-6

   Expression

   Bacterial

   Mutation

   Contains PP1 alpha residues 7-330

   Promoter

   T7-lac

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCellFree_G03 IL1B

   Plasmid

   #67066

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   IL1B (IL1B Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pETM6-Amp-T7-roGFP

   Plasmid

   #202464

   Purpose

   Expresses redox sensitive GFP (roGFP) with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   reduction-oxidation sensitive green fluorescent protein
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNC1125

   Plasmid

   #41561

   Depositor

   Beverly Errede

   Article

   Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

   Insert

   Ura3-GAL1-UBIMdkGFP*-SpHis5-Tim9
   

   Use

   Tags

   Expression

   Mutation

   GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

   Promoter

   Available Since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. human p53-(1-393)-N268D

   Plasmid

   #24861

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Asparagine 268 mutated to Aspartate

   Promoter

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pCellFree_G03 CALR

   Plasmid

   #67086

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CALR (CALR Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET28a-SENP5 (catalytic domain)

   Plasmid

   #16358

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP5 catalytic domain (SENP5 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 567 to 755 catalytic domain of human S…

   Promoter

   Available Since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase

    Plasmid

    #25846

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA-W37I
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    W37I

    Promoter

    Available Since

    July 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDEST40-Arl13b11

    Plasmid

    #40873

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    no stop codon

    Promoter

    CMV

    Available Since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

12. human p53-Δ(325-355)

    Plasmid

    #24871

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Deletion of aa 325-355

    Promoter

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pMCSG28-SH3-588

    Plasmid

    #185920

    Purpose

    Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase

    Depositor

    Brian Kuhlman

    Article

    Park et al Nat Chem Biol. 2023 Apr;19(4):460-467. d

    Insert

    SH3-588
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Designed enzyme with novel interface between huma…

    Promoter

    T7

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEBG-TFII-I delta isoform

    Plasmid

    #22188

    Depositor

    Ananda Roy

    Article

    Cheriyath et al Mol Cell Biol. 1998 Aug . 18(8):4444-54.

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDFBB-eGFP

    Plasmid

    #32550

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    egfp (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    Consitutive lac

    Available Since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTorPE-IP2.0

    Plasmid

    #109217

    Purpose

    Express IP2.0 in E. coli

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUB6-VSV-GS

    Plasmid

    #190781

    Purpose

    Expresses VSV-GS in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

    Insert

    VSV-GS
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQTEV-PEPD

    Plasmid

    #31328

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    peptidase D (PEPD Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCellFree_G04

    Plasmid

    #67138

    Purpose

    Gateway destination vector for cell-free expression with C-term eGFP 8xHis tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    EGFP and HIS

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. human p53-(310-360)

    Plasmid

    #24872

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains only the dimeric oligomerization domain …

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pACBB-eGFP

    Plasmid

    #32551

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    Consitutive lac

    Available Since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEBG-TFII-I-p70

    Plasmid

    #22187

    Depositor

    Ananda Roy

    Article

    Cheriyath et al Mol Cell Biol. 1998 Aug . 18(8):4444-54.

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Contains only amino acids 1-735 C-terminal residu…

    Promoter

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pThuS

    Plasmid

    #171674

    Purpose

    ThuS expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    Glycosyltransferase ThuS
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. NTSP-PC1

    Plasmid

    #41575

    Depositor

    Thomas Weimbs

    Article

    Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    Myc/Myc/His

    Expression

    Mammalian

    Mutation

    4106-4218 of FLS PC1

    Promoter

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

    Plasmid

    #87350

    Purpose

    S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/S355C/C574S/S867C

    Promoter

    T7

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTriEx4-H6-FGB

    Plasmid

    #49203

    Purpose

    Fluorescent reporter for cGMP

    Depositor

    John Garthwaite

    Article

    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

    Insert

    H6-FGB
    

    Use

    Tags

    His tag + Other tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    None

    Promoter

    CMV

    Available Since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET22b-T5-FB-E25TAG

    Plasmid

    #176545

    Purpose

    Expresses IgG affinity protein FB with a TAG stop codon at E25 position

    Depositor

    Han Xiao

    Article

    Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

    Insert

    IgG affinity protein
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    change glutamic acid 25 to amber stop codon

    Promoter

    T5

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MSX2-pET

    Plasmid

    #21025

    Depositor

    Corey Largman

    Article

    Stelnicki et al Differentiation. 1997 Oct . 62(1):33-41.

    Insert

    MSX2 (Msx2 Mouse)
    

    Use

    Tags

    HIS and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

29. Lmajor_gp63_10_0460_E265A

    Plasmid

    #171641

    Purpose

    Expression of catalytically inactive L. major glcyoprotein-63 (E265A) from chromosome 10 in mammalian cells

    Depositor

    Dennis Ko

    Article

    Antonia et al Front Cell Infect Microbiol. 2019 Aug 7;

    Insert

    LmjF.10.0460 E265A
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    E265A

    Promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSP72-deltaFlincG

    Plasmid

    #19733

    Depositor

    Wolfgang Dostmann

    Article

    Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    delta-FlincG (PRKG1 Bovine)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 77-356

    Promoter

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

31. pET15b-hnRNP-F

    Plasmid

    #23021

    Depositor

    Douglas Black

    Article

    Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

    Insert

    hnRNP-F (HNRNPF Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

32. pCellFree_G07

    Plasmid

    #67141

    Purpose

    Gateway destination vector for cell-free expression with N-term myc-tag mCherry tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His-MYC and mCherry

    Expression

    Mutation

    Promoter

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Halo-Tev-Akt3 WT-2X Flag

    Plasmid

    #87607

    Purpose

    expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of human Akt3 protein

    Depositor

    Yimon Aye

    Insert

    Akt3 isoform 2 (AKT3 Human)
    

    Use

    Zebrafish (danio rerio) expression

    Tags

    2 tandem flag tags and His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Akt3 isoform 2

    Promoter

    SP6

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMS32: pTwinStrep-SUMO-PmcTnsC

    Plasmid

    #168165

    Purpose

    PmcTnsC expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST TnsC
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EKAR2G_design1_mTFP_175_Venus_173

    Plasmid

    #39830

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pTriExEKAR2G1

    Plasmid

    #40174

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pT7-agrBD-I

    Plasmid

    #53439

    Purpose

    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrBD locus
    

    Use

    Synthetic Biology

    Tags

    V5 epitope, 6x HIS (not expressed on agrB or agrD…

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7-lac

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEBG-TFII-I gamma

    Plasmid

    #22156

    Depositor

    Ananda Roy

    Article

    Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

39. pET-28a+GFP-CAMSAP2 CKK

    Plasmid

    #59032

    Purpose

    expresses CKK domain of CAMSAP2 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP2 CKK domain (CAMSAP2 Human)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pFR-mTq2-antiFRET-P2A-jRCaMP1b

    Plasmid

    #191468

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    jRCaMP1b
    

    Use

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge