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Showing: 11851 - 11900 of 23156 results

  3. p426_Cas9_gRNA-ARS720a

    Plasmid
    #87392
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS720a sequence CAACAATTGTTACAATAGTA in yeast chromosome 7.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS720a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87392-plasmid-map-sequence-id-193435

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  4. p426_Cas9_gRNA-ARS1021b

    Plasmid
    #87395
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1021b sequence CCTCTGTGTGGTGGTAATTG in yeast chromosome 10.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1021b
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87395-plasmid-map-sequence-id-193438

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  5. p426_Cas9_gRNA-ARS1014a

    Plasmid
    #87396
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87396-plasmid-map-sequence-id-193439

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  6. pMXs-hs-3xHA-C12AC15A-LIN-41

    Plasmid
    #52725
    Purpose
    retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations at cysteine 12 and 15 and 3 N-terminal HA tags
    Depositor
    Shinya Yamanaka
    Article
    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.
    Insert
    LIN-41 (TRIM71 Human)
    Use
    Retroviral
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    changed cysteines 12 and 15 to alanines
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    52725-plasmid-map-sequence-id-82094

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  11. p426_Cas9_gRNA-YOLCd1b

    Plasmid
    #87401
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YOLCd1b sequence GACTAGTTAAGCGAGCATGT in yeast chromosome 15.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting YOLCd1b
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87401-plasmid-map-sequence-id-193444

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  12. p426_Cas9_gRNA-CAN1y

    Plasmid
    #87391
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting CAN1y sequence GATACGTTCTCTATGGAGGA in yeast chromosome 6.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting CAN1y
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87391-plasmid-map-sequence-id-193434

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  16. pCHC030 (∆CBBp ∆MBH ∆SH)

    Plasmid
    #226205
    Purpose
    Electroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for CBBp, MBH, and SH operons, and intervening sequencessequences.
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ∆cbbR ∆cbbLSXYEFPTZGKA ∆hoxKGZMLOQRTV ∆hypA1B1F1C…
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226205-plasmid-map-sequence-id-453511

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  17. pCHC036 (∆pHG1)

    Plasmid
    #226206
    Purpose
    Conjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for the pHG1 megaplasmid replication region
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ∆helD ∆repA ∆repB ∆parAB
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226206-plasmid-map-sequence-id-452437

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  25. psiCHECK-1-miR-376a1(3p)-I-type

    Plasmid
    #215883
    Purpose
    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
    Depositor
    Kumiko Ui-Tei
    Article
    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1
    Insert
    three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    215883-plasmid-map-sequence-id-443531

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  29. psiCHECK-1-miR-376a2(3p)-I-type

    Plasmid
    #215887
    Purpose
    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
    Depositor
    Kumiko Ui-Tei
    Article
    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1
    Insert
    three tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-I-type (MIR376A2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    215887-plasmid-map-sequence-id-443595

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  35. pGBT9-ATG9A-N

    Plasmid
    #198530
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11
    Insert
    ATG9A N-terminal cytosolic tail (residues 1-66) (ATG9A Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198530-plasmid-map-sequence-id-389380

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  36. pGADT7-δ

    Plasmid
    #197259
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-3 δ (AP3D1 Human)
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Val 549 Ile substitution; silent substitutions in…
    Promoter
    ADH1
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197259-plasmid-map-sequence-id-387016

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  37. pBridge-tyrosinase.σ3A

    Plasmid
    #197513
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-3 σ3A
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    silent substitution in codon 2 of tyrosinase tail…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197513-plasmid-map-sequence-id-388877

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  42. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1mut-F961A_S

    Plasmid
    #147514
    Purpose
    Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-F961A
    Depositor
    Elisa Izaurralde
    Article
    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94
    Insert
    CeAIN2_254-400-DmGW182_635-1384-CIM1-F961A (gw Human, Nematode)
    Use
    Tags
    Expression
    Insect
    Mutation
    Deletion of 2 AA (SD) at position 378-379 compare…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147514-plasmid-map-sequence-id-289961

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  47. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut_R

    Plasmid
    #147388
    Purpose
    Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut
    Depositor
    Elisa Izaurralde
    Article
    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94
    Insert
    CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut (gw Fly, Nematode)
    Use
    Tags
    Expression
    Insect
    Mutation
    Deletion of 2 AA (SD) at position 378-379 compare…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147388-plasmid-map-sequence-id-289835

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Showing: 11851 - 11900 of 23156 results