We narrowed to 149,122 results for: ins;

1. pLVX-RT006-GCN4-HaloPM

   Plasmid

   #163386

   Purpose

   To express 4X sGCN4 module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   4XGCN4-Halotag-PDGFRtm-2A-mCherry (GCN4-HaloPM)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyCatcher002-oPent

   Plasmid

   #124664

   Purpose

   Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oPent
   

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac1 PIK3R1

   Plasmid

   #17743

   Depositor

   Bert Vogelstein

   Article

   Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

   Insert

   PIK3R1 (PIK3R1 Human)
   

   Expression

   Insect

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pAct:dCas9-GCN4

   Plasmid

   #78899

   Purpose

   Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-10XGCN4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Promoter

   pActin (Drosophila)

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2ADWpuro_2paRAF

   Plasmid

   #129653

   Purpose

   For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-K-I-N-CAAX

   Plasmid

   #41640

   Purpose

   Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

   Insert

   Dronpa145K-IntersectinDH-Dronpa145N-CAAX
   

   Tags

   CAAX box

   Expression

   Mammalian

   Mutation

   K145N on Dronpa

   Promoter

   CMV

   Available Since

   Dec. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti X2 Zeo DEST (749-3)

   Plasmid

   #21562

   Purpose

   3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR insertion, Zeo

   Depositor

   Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-rTH-tTA

   Plasmid

   #133268

   Purpose

   An AAV genome that expresses tTA from the rat TH promoter

   Depositor

   Viviana Gradinaru

   Article

   Robinson et al Elife. 2019 Sep 23;8. pii: 48983. doi: 1

   Insert

   tTA
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   rTH

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAct:dCas9

   Plasmid

   #78903

   Purpose

   Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   pActin (Drosophila)

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpyCatcher002-oHept

    Plasmid

    #124671

    Purpose

    Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oHept
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLBac-FluB-trimer

    Plasmid

    #229859

    Purpose

    Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

    Depositor

    Stephen Cusack

    Article

    Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

    Inserts

    PA, Influenza B (B/Memphis/13/03)
    PB1, Influenza B (B/Memphis/13/03)
    PB2, Influenza B (B/Los Angeles/1/02)
    

    Tags

    2x-Streptag and 8xHis-tag

    Expression

    Insect

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCOLA banana sensor sfGFP

    Plasmid

    #107367

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    banana-detecting toehold + sfGFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLIC-lyn11-myc-LOV2GIVe

    Plasmid

    #170623

    Purpose

    For the mammalian expression of mLOV2GIVe

    Depositor

    Mikel Garcia-Marcos

    Article

    Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

    Insert

    Lyn11-myc-LOV2GIVe
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. MSP1594

    Plasmid

    #65775

    Purpose

    Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSN007

    Plasmid

    #102440

    Purpose

    For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

    Depositor

    Alejandro Chavez, George Church

    Article

    Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

    Insert

    7SK paired gRNA insert
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. vas-Cas9.874Z

    Plasmid

    #112687

    Purpose

    Express hSpCas9 under vasa promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    vasa

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-PI(WT)-Src(WT)-mCherry

    Plasmid

    #87356

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src (Src Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLinker-AID-3xHA-DHFR-LoxP

    Plasmid

    #86669

    Purpose

    for generation of AID strains using CRISPR tagging technology

    Depositor

    David Sibley

    Article

    Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

    Insert

    Linker-AID-3xHA-HXGPRT 3'UTR
    

    Use

    Expression in toxoplasma gondii

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXylA-agrCA-I

    Plasmid

    #53437

    Purpose

    xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrCA locus
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    "MVQTS" sequence added to the N-terminu…

    Promoter

    PxylA

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. Htb2-GFP

    Plasmid

    #117669

    Purpose

    Colorize the nuclei

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    Htb2-GFP
    

    Expression

    Yeast

    Mutation

    Contains the gene encoding the Histone Htb2 from …

    Promoter

    pGAP

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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