We narrowed to 4,899 results for: phy

1. IDA3::NeonGreen N-term (WS14)

   Plasmid

   #126875

   Purpose

   NeonGreen-tag attached to the N-terminus of IDA3. Includes ampicilin and hygromycin resistance.

   Depositor

   Winfield Sale

   Article

   Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

   Insert

   IDA3
   

   Use

   Other

   Tags

   NeonGreen

   Expression

   Mutation

   Promoter

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. IDA3::NeonGreen exon2 (WS15)

   Plasmid

   #126876

   Purpose

   NeonGreen-tag embedded in the second exon of IDA3. Includes ampicilin and hygromycin resistance.

   Depositor

   Winfield Sale

   Article

   Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

   Insert

   IDA3
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAS

   Plasmid

   #206994

   Purpose

   Plasmid expressing both SpCas9 and SaCas9

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Insert

   SpCas9 - P2A - SaCas9 (NEWENTRY, NEWENTRY )
   

   Use

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Luciferase-pcw107-V5

   Plasmid

   #64649

   Purpose

   (control) when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   n/a
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   PGK

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCPP3373

   Plasmid

   #128718

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopQ1-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Synonymous point mutation in the wobble position…

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFsynW SYP-GFP

   Plasmid

   #197283

   Purpose

   Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYP and GFP (Syp Rat)
   

   Use

   Lentiviral

   Tags

   1D4 epitope

   Expression

   Mutation

   N/A

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. eDrIR1

   Plasmid

   #183998

   Purpose

   Optically controlled eDrIR1 expression in mammalian cells

   Depositor

   Vladislav Verkhusha, Dmitry Gerashchenko

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrIR1 (INSR Human)
   

   Use

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hsyn-DIO-GCaMP6s

   Plasmid

   #184284

   Purpose

   Expresses GCaMP6s in genetically defined neurons expressing Cre recombinase

   Depositor

   William Wisden

   Article

   Yu et al Nat Neurosci. 2019 Jan;22(1):106-119. do

   Insert

   CCaMP6s
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   human synaptophysin

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ai34

   Plasmid

   #34881

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

   Insert

   CAG-Floxed Synaptophysin-tdTomato
   

   Use

   Mouse Targeting

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   CAG

   Available since

   March 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Affibody HER2:243.BirA*-pET301/NT-DEST

    Plasmid

    #174692

    Purpose

    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*

    Depositor

    Jean Gariepy

    Article

    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

    Insert

    Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
    

    Use

    Tags

    HIS tag

    Expression

    Bacterial

    Mutation

    R118G mutation in BirA

    Promoter

    T7 promoter

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCPP5233

    Plasmid

    #128715

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

    Insert

    shcM-hopM1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Kv7.5/pcDNA3.1

    Plasmid

    #111498

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCPP5060

    Plasmid

    #128716

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

    Insert

    shcN-hopN1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV2/9-CW3SL-mCherry-DrTrkB

    Plasmid

    #184002

    Purpose

    AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrTrkB (NTRK2 Human)
    

    Use

    AAV

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Luciferase-pcw107

    Plasmid

    #64648

    Purpose

    (control) when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    n/a
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pRiboI-APEX2m

    Plasmid

    #176843

    Purpose

    Expression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)

    Depositor

    Jessica Seeliger

    Article

    Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

    Insert

    pRibo-APEX2m
    

    Use

    Integrating

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRibo, theophilline riboswitch inducible hsp60 pr…

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-CreLite

    Plasmid

    #131786

    Purpose

    Lentiviral vector expressing CreLite, driven by CBh promoter

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PhyBCreC-P2A-PIF6CreN
    

    Use

    Cre/Lox, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCA528 SPASTIN , residues 112-196

    Plasmid

    #180627

    Purpose

    Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 112-196

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV hSyn1 ChR2 ET-TC 2A tDimer

    Plasmid

    #101361

    Purpose

    High efficiency channelrhodopsin, neuron specific promoter, red fluorescent protein

    Depositor

    Thomas Oertner

    Article

    Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

    Inserts

    Channelrhodopsin-2
    synaptophysin-red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E123T , T159C

    Promoter

    human Synapsin

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRibo-APEX2m

    Plasmid

    #176842

    Purpose

    Expression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)

    Depositor

    Jessica Seeliger

    Article

    Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

    Insert

    pRibo-APEX2m
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRibo, theophilline riboswitch inducible hsp60 pr…

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge