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pR-mPapaya

Plasmid

#228607

Purpose

Mammalian expression of mPapaya, for MuSIC barcode construction

Depositor

Marc Birtwistle

Publication

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mPapaya

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mClover3

Plasmid

#228624

Purpose

Mammalian expression of mClover3 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Publication

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mClover3

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDonorTp-fugfp

Plasmid

#211790

Purpose

pDonorTp with addition of fugfp gene from pUS252 outside of the FRT sites

Depositor

Silvia Cardona

Publication

Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

Insert

fuGFP

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-empty

Plasmid

#221239

Purpose

Expression of FLAG-4xFKBP scaffold in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

FLAG-4xFKBP scaffold

Use

Retroviral

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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aCE120

Plasmid

#204491

Purpose

Figure 1. reporter circuit

Depositor

Ron Weiss

Publication

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

EBFP2
mKate2
EYFP

Use

Synthetic Biology

Tags

none

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pPath-pR-integrase

Plasmid

#200000

Purpose

Entry vector for biosynthetic pathway. pR-phiC31 integrase

Depositor

Farren Isaacs

Publication

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRset-ARSeNL

Plasmid

#207872

Purpose

Bacterial expression of the BRET ATP sensor

Depositor

Mathew Tantama

Publication

Min et al Sensors (Basel). 2019 Aug 10;19(16). pii

Insert

ARSeNL

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-HIV-1_PR

Plasmid

#203493

Purpose

Expresses HIV-1 protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HIV-1 protease

Expression

Yeast

Promoter

GALL

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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PMT-mApple

Plasmid

#203771

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Publication

Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

Insert

PMT-mApple

Expression

Mammalian

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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sgCTRL-hygro

Plasmid

#199639

Purpose

sgRNA control; induces CAS9 cutting in an intergenic region

Depositor

William Hahn

Publication

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0001

Plasmid

#196141

Purpose

Gateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Publication

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtTGA1_HiBit

Use

Gateway Cloning

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCdKN0001

Plasmid

#196148

Purpose

Gateway destination vector for protein expression of 9xHis_AtTGA1_HiBit

Depositor

Nicola Patron

Publication

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

9xHis_AtTGA1_HiBit

Expression

Bacterial

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_A5803

Plasmid

#176878

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Publication

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV:Luc+:HSP

Use

Luciferase

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3

Plasmid

#120253

Purpose

Expression of ATP3 in yeast

Depositor

Markus Ralser

Publication

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Expression

Yeast

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP5V1

Plasmid

#165058

Purpose

Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP

Depositor

Claudia Vickers

Publication

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1- T-URA3

Expression

Yeast

Mutation

WT

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJAID6A

Plasmid

#165046

Purpose

Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

Depositor

Claudia Vickers

Publication

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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Rap-MESA-20-M-Gal4

Plasmid

#84554

Purpose

MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

Depositor

Joshua Leonard

Publication

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FRB-20-M-Gal4

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pPD301 ZF43x16-Compact_EYFP

Plasmid

#138911

Purpose

EYFP reporter vector for the ZF43x16-Compact promoter

Depositor

Joshua Leonard

Insert

ZF43x16-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x16-Compact

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPD299 ZF43x14-Compact_EYFP

Plasmid

#138909

Purpose

EYFP reporter vector for the ZF43x14-Compact promoter

Depositor

Joshua Leonard

Insert

ZF43x14-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x14-Compact

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPD294 ZF43x10-Compact_EYFP

Plasmid

#138905

Purpose

EYFP reporter vector for the ZF43x10-Compact promoter

Depositor

Joshua Leonard

Insert

ZF43x10-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x10-Compact

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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