1,878 results

1. pXF222

   Plasmid

   #139032

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   mkate2 (no STOP codon)
   

   Expression

   Worm

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGC76

   Plasmid

   #19641

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   <4xNLS-GFP::let-858(3’)
   

   Use

   Flp/frt

   Tags

   4xNLS

   Expression

   Worm

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJH3666

   Plasmid

   #191447

   Purpose

   Pacr-2s-GCaMP6s::wCherry unc-54 3' UTR C.elegans A/B MN expression of GCaMP6s RFP

   Depositor

   Mei Zhen

   Article

   Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

   Insert

   GCaMP6s
   

   Tags

   wCherry

   Expression

   Worm

   Promoter

   Pacr-2s

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJH3043

   Plasmid

   #175284

   Purpose

   Pdpy-30 DAF-16a unc-54 3' UTR C.elegans pan-tissue expression of isoform a of DAF-16 fused to GFP

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   daf-16a mini-gene (daf-16 Nematode)
   

   Expression

   Worm

   Promoter

   Pdpy-30

   Available Since

   Nov. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAP546-2

   Plasmid

   #99476

   Purpose

   3XFlag::mNeptune::Myc

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   3XFlag::mNeptune::Myc
   

   Expression

   Bacterial

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)

   Plasmid

   #154838

   Purpose

   Insertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selection

   Depositor

   Patrick Phillips

   Article

   Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

   Insert

   synthetic guide::∆HYGR::unc-54 3' UTR
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   ∆HYGR is promoterless and encodes aa 60-341

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPD162.21

   Plasmid

   #27511

   Depositor

   Andrew Fire

   Article

   Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

   Insert

   pmyo-3::dsRed::let-858_3'UTR
   

   Expression

   Worm

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHA843 - hrp-1p::hrp-1HsLCdeltaLC

   Plasmid

   #139200

   Purpose

   Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Expression

   Worm

   Mutation

   stop at beginning of last exon

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEY68

   Plasmid

   #191065

   Purpose

   ceh-14 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   ceh-14 (ceh-14 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEY61

    Plasmid

    #191058

    Purpose

    acr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-2(3.7k) (acr-2 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET_RGG-RGG_withTEVsite

    Plasmid

    #124940

    Purpose

    Expression of RGG-RGG

    Depositor

    Daniel Hammer, Matthew Good, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    with TEV site between RGG domains

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCZGY553

    Plasmid

    #110883

    Purpose

    Pmec-4 Gateway Destination vector for cloning for expression in C. elegans mechanosensory neurons

    Depositor

    Andrew Chisholm

    Article

    Ghosh-Roy et al J Neurosci. 2010 Mar 3;30(9):3175-83. do

    Insert

    Pmec-4
    

    Use

    Gateway destination vector

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCM5.50

    Plasmid

    #19383

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    spn-4 3'utr in pDONRP2RP3 (spn-4 Nematode)
    

    Expression

    Worm

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGC49

    Plasmid

    #19680

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Gateway(R1-R2)-L4440
    

    Use

    RNAi; Gateway destination vector

    Available Since

    Feb. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJW1882

    Plasmid

    #154330

    Purpose

    sgRNA Target Vector for CRISPR

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    ttTi4348 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. nud-1::L4440

    Plasmid

    #11337

    Depositor

    Guy Caldwell

    Insert

    nud-1 (nud-1 Nematode)
    

    Use

    RNAi

    Expression

    Worm

    Available Since

    July 12, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pAS1-his-24::tagRFP

    Plasmid

    #124329

    Purpose

    Expressing Nuclear Localized tagRFP

    Depositor

    Andrew Leifer

    Insert

    tagRFP (his-24 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNM3649

    Plasmid

    #154110

    Purpose

    miniMos dual component RMCE landing site vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    loxP::SEC::loxP::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
    

    Use

    Minimos

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGC133

    Plasmid

    #19612

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Phsp-16.41::FLP::let-858(3’)-(Cbr)unc-119(+)
    

    Use

    Flp/frt

    Expression

    Worm

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCG26

    Plasmid

    #26938

    Depositor

    Geraldine Seydoux

    Article

    Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

    Insert

    dcap-1 (dcap-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Available Since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pCM1.111

    Plasmid

    #19598

    Depositor

    Geraldine Seydoux

    Insert

    mex-5 prom in PDONRP4P1R
    

    Expression

    Worm

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJH2947

    Plasmid

    #132362

    Purpose

    Prgef-1 INS-2::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-2::GFP

    Depositor

    Mei Zhen

    Article

    Hung et al Development. 2014 Apr;141(8):1767-79. do

    Insert

    INS-2 (ins-2 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Prgef-1

    Available Since

    Oct. 7, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. pJN601

    Plasmid

    #59790

    Purpose

    zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a marker

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    ZF1 domain from pie-1
    N-terminal half of yfp with introns
    intron containing floxed unc-119 gene in reverse orientation
    C-terminal half of yfp with introns
    

    Expression

    Worm

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. KG#470

    Plasmid

    #110930

    Purpose

    Expresses proteins pan-neuronally in C. elegans (entire nervous system)

    Depositor

    Kenneth Miller

    Article

    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

    Inserts

    rab-3 promoter
    unc-54 3' control region
    

    Expression

    Bacterial

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEY64

    Plasmid

    #191061

    Purpose

    aqp-6 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    aqp-6 (aqp-6 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCM5.44

    Plasmid

    #19389

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    cep-1 3'utr in pDONRP2RP3
    

    Expression

    Worm

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET_Unc653_GFP_His

    Plasmid

    #14107

    Depositor

    Ron Vale

    Article

    Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

    Insert

    Unc104 (1-653) (unc-104 Nematode)
    

    Tags

    GFP and His

    Expression

    Bacterial

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pRG02

    Plasmid

    #44196

    Depositor

    Denis Dupuy

    Insert

    Neo
    

    Expression

    Worm

    Promoter

    rps-27

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BSP602

    Plasmid

    #122249

    Purpose

    expression vector for mScarlet under EFT3 Promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mScarlet
    

    Use

    Synthetic Biology

    Expression

    Worm

    Promoter

    EFT3

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCFJ66

    Plasmid

    #24981

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2010 Jun;7(6):451-3. doi: 1

    Insert

    Cbr-unc-119 Punc-122::GFP
    

    Use

    Multisite gateway

    Available Since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge