We narrowed to 7,632 results for: ALP
-
Plasmid#163372PurposeExpress HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser345Ala) plasmidDepositorInsertHypoxia Inducible Factor 2 alpha (EPAS1 Human)
TagsHA- and Clover-taggedExpressionMammalianMutationS345APromoterCMV PromoterAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-1alpha S31D
Plasmid#163370PurposeExpress HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser31Asp) plasmidDepositorInsertHypoxia Inducible Factor 1 alpha S31D (HIF1A Human)
TagsHA- and Clover-taggedExpressionMammalianMutationS31DPromoterCMV PromoterAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-1alpha S31G
Plasmid#163369PurposeExpress HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser31Gly) plasmidDepositorInsertHypoxia Inducible Factor 1 alpha S31G (HIF1A Human)
TagsHA- and Clover-taggedExpressionMammalianMutationS31GPromoterCMV PromoterAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-1alpha S786D
Plasmid#163368PurposeExpress HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser786Asp) plasmidDepositorInsertHypoxia Inducible Factor 1 alpha S786D (HIF1A Human)
TagsHA- and Clover-taggedExpressionMammalianMutationS786DPromoterCMV PromoterAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover-HIF-1alpha S786A
Plasmid#163367PurposeExpress HA- and Clover-tagged HIF-1alpha (with a site-directed mutation Ser786Ala) plasmidDepositorInsertHypoxia Inducible Factor 1 alpha S786A (HIF1A Human)
TagsHA- and Clover-taggedExpressionMammalianMutationS786APromoterCMV PromoterAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha2 P35S:RDF:T35S (GB1508)
Plasmid#160613PurposeTU for the constitutive expression of Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)DepositorInsertRDF
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)
Plasmid#160614PurposeTU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.DepositorInsertPhiC31
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromotermini35sAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)
Plasmid#160615PurposeTU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.DepositorInsertPhiC31
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromotermini35sAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL-C2X -MTalpha-FliG
Plasmid#154361PurposeBacterial expression of MtalphaDepositorInsertMtalpha
ExpressionBacterialAvailable SinceNov. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE (hygro) hYAP 1-1alpha
Plasmid#124147PurposeProtein expression for funtional studies of cell growth promotionDepositorAvailable SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE (hygro) hYAP 1-2alpha
Plasmid#124151PurposeProtein expression for funtional studies of cell growth promotionDepositorAvailable SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS puro miR30-TAK1
Plasmid#117162PurposeTarget site AGCGCCCTTCAATGGAGGAAATDepositorInsertshRNA targeting miR30
UseLentiviralAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS puro miR30-STAT2
Plasmid#117161PurposeTarget site TTTAAGTTCCACAGACTTGGADepositorInsertshRNA targeting miR30
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4H
Plasmid#108374PurposeExpresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertExpressionMammalianPromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Alpha-Actinin-19
Plasmid#57782PurposeLocalization: Alpha Actinin, Excitation: 448 / 473, Emission: 500 / 528DepositorAvailable SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-CFP
Plasmid#50482Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
TagsCFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the CFP open…PromoterCMVAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-alpha4-SEP
Plasmid#45607DepositorInsertnAChRAlpha4-SEP
TagsSEP- TagExpressionMammalianPromoterCMVAvailable SinceAug. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2alpha D291N mutant
Plasmid#128813PurposeTo make virus expressing Doc2alpha D291 mutantDepositorInsertDoc2alpha (Doc2a Rat)
UseLentiviralTagsIRES-EGFPMutationD291NPromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-deltaTMD SYT7alpha
Plasmid#179718PurposeLentiviral expression of synaptic vesicle-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD
Plasmid#179722PurposeLentiviral expression of lysosome-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralAvailabilityAcademic Institutions and Nonprofits only -
Px461-Cas9n-Trp53-sgRNA-alpha
Plasmid#88846PurposeCRISPR KO of Trp53DepositorAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
Plasmid#71396Purpose2Gi2R assayDepositorInserts2xGFP:NES
2xRFP:NLS
UseLentiviralTagsIRESExpressionMammalianPromoterEF1alpha and IRESAvailable SinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-VP64-dCas9-VP64_Blast
Plasmid#192650Purpose3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance markerDepositorInsertVP64-dCas9-VP64
UseCRISPR and LentiviralExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 Alpha Spike
Plasmid#185691PurposeExpresses codon-optimized full length SARS-CoV-2 Alpha SpikeDepositorInsertSARS-CoV-2 Alpha Spike (S )
ExpressionMammalianMutationContains the following mutations: Δ69-70, Δ145, N…Promoterchicken β-actin promoter, CMV enhancerAvailable SinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSV human p85 alpha (HA tag)
Plasmid#11499DepositorAvailable SinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
PGC-1 alpha promoter 2kb luciferase
Plasmid#8887DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 GFP B55 alpha shRNA 3-1
Plasmid#10679DepositorAvailable SinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha P402A/P564A-pBabe-puro
Plasmid#19005DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 402 to Alanin…Available SinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
Anti-GABA(A)R, Alpha1 [N95/35R]
Plasmid#177574PurposeMammalian Expression Plasmid of anti-GABA(A)R, Alpha1 (Human). Derived from hybridoma N95/35.DepositorInsertanti-GABA(A)R, Alpha1 (Homo sapiens) recombinant mouse monoclonal antibody (GABRA1 Mouse)
ExpressionMammalianPromoterDual CMVAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2alpha-P405A/P531A-pBabe-puro
Plasmid#19006DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: P405A and P531AAvailable SinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
Slo1/BKAlpha maxi-K+ channel scFv [L6/60]
Plasmid#199421PurposeMammalian expression of Slo1/BKAlpha maxi-K+ channel scFv. Derived from hybridoma L6/60.DepositorInsertrecombinant mouse scFv targetingSlo1/BKAlpha maxi-K+ channel (Rat) (Kcnma1 Mouse)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-E2-Crimson
Plasmid#117661PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-Ost1-alphaf(I)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro
Plasmid#192664Purpose3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance markerDepositorInsertdCas9-KRAB-MeCP2
UseCRISPR and LentiviralExpressionMammalianMutationD10A, D839A, H840A and N863A in Cas9PromoterEF1aAvailable SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)
Plasmid#160626PurposeTU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domainsDepositorInsertP35s-dCas9:TV-Tnos
ExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-TNFalpha(Ozoralizumab) PAGER(Gq)
Plasmid#230015PurposeExpresses anti-TNFalpha PAGER(Gq) in mammalian cellsDepositorInsertMT1-Ozoralizumab-TEVcs-ALFA-hM1D
UseAAVExpressionMammalianMutationW442A on hM1DPromoterCMVAvailable SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
Stat1 alpha Flag pRc/CMV
Plasmid#8691DepositorAvailable SinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf(I)-E2-Crimson
Plasmid#117660PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf(I)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-VP64-dCas9-p300_Blast
Plasmid#192654Purpose3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance markerDepositorInsertVP64-dCas9-p300
UseCRISPR and LentiviralExpressionMammalianMutationD10A; H840A in Cas9PromoterEF1aAvailable SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-Btl2
Plasmid#117667PurposeStudy secretion efficiency of Btl2DepositorInsertpre-Ost1-alphaf(I)-Btl2
ExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-mouse PKA-RIalpha-mEGFP
Plasmid#45525DepositorInsertPKA regulatory subunit RI alpha tagged by mEGFP
TagsHisExpressionMammalianAvailable SinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits only