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Plasmid#89346Purpose2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassetteDepositorInsertTCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
UseLentiviralTagsSNAPf tagExpressionMammalianMutationPromoterSFFVAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
hBACCS2-IRES-GFP
Plasmid#72891PurposeMammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and GFPDepositorInserthBACCS2-IRES-GFP
UseTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo3+GFP-Utr_CH
Plasmid#64359Purposebicistronic expression of GFP-Utrophin and Neo resistanceDepositorInsertGFP-Utrophin (UTRN Human)
UseTagsGFP and IRES-NeoExpressionMammalianMutationQ236R; aa1-261 Utrophin presentPromoterCMVAvailable sinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo3+mRFP-Utr_CH
Plasmid#64360Purposebicistronic expression of mRFP-Utrophin and Neo resistanceDepositorInsertmRFP-Utrophin (UTRN Human)
UseTagsIRES-Neo and mRFPExpressionMammalianMutationQ236R; aa1-261 Utrophin presentPromoterCMVAvailable sinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
DNA-CARzeta-IRESpuro
Plasmid#89345Purpose2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassetteDepositorInsertDNA-CARzeta
UseLentiviralTagsSNAPf tagExpressionMammalianMutationPromoterAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
[Bla][IRES][GFP]
Plasmid#97193PurposeVector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.DepositorInsertsbeta-Lactamase
GFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR-NLS
Plasmid#118467Purposecp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.DepositorInsertsapphireAKAR-NLS
UseTagsNuclear localization signal (NLS)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-RFP-IRES-CD
Plasmid#126678PurposeDoxycycline inducible expression of CD along with reporter RFPDepositorInsertRFP-IRES-CD (FCY1 Budding Yeast)
UseTagsExpressionMutationPromotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjBCCIP
Plasmid#122278PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjBCCIP 3'UTR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113344PurposeExpresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteinsDepositorInsertVP64-dCas9-p300core-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMutationPromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113342PurposeExpresses dCas9-p300 and MS2-p65-HSF1 fusion proteinsDepositorInsertdCas9-p300core-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA
Plasmid#112921PurposeGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant markerDepositorInsertEF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
PU.1-ets-IRES-mCherry
Plasmid#80141Purposeretroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherryDepositorInsertPU.1 (Spi1 Mouse)
UseRetroviralTagsIRES-mCherryExpressionMammalianMutationets = PU.1 DNA binding domain (amino acids 159-26…PromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSIREN-RetroQ-shLuc
Plasmid#73666PurposepSRIEN-RetroQ vector containing control sequence luciferaseDepositorInsertshRNA to Luciferase
UseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQUAST-shibire (ts1)
Plasmid#24362DepositorInsertShibire (ts1) (shi Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-sapphireAKAR
Plasmid#118466Purposecp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.DepositorInsertLyn-sapphireAKAR
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-siResist
Plasmid#49852PurposeEncodes human connexin 43 with wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationContains several wobble mutations to confer resis…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
hBACCS2-IRES-mCherry
Plasmid#72892PurposeMammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherryDepositorInserthBACCS2-IRES-mCherry
UseTagsIRES-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-Firefly-ccdB
Plasmid#87077PurposeLentiviral Gateway-compatible expression vector backbone with an N-terminal Firefly luciferaseDepositorTypeEmpty backboneUseLentiviralTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMYs-IRES-mitoRFP
Plasmid#121996PurposeExpression of mitoRFP in mammalian cellsDepositorInsertIRES-mitoRFP
UseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only