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Plasmid#196972PurposeBacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tagDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTags6xHis and GFPExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT1
Plasmid#53557Purposebacterial expression of IFIT1DepositorInsertIFIT1 (IFIT1 Human)
UseTags6xHIS and TEVExpressionBacterialMutationPromoterT7Available sinceMay 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a_DmKHC[1-421]-SNAP-6xHis
Plasmid#196975PurposeBacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tagDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTags6xHis and SNAPExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-AsCas12f-2NLS
Plasmid#171613PurposeNLS-AsCas12f1-NLS heterologous expression plasmid for RNPDepositorInsertNLS-AsCas12f1-NLS
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP6 (catalytic domain)
Plasmid#16359DepositorInserthuman SENP6 catalytic domain (SENP6 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 628 to 1112 catalytic domain of human …PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-StrepII-TagRFP-CohI
Plasmid#58710PurposeDetection construct to label dockerin type I containing fusion proteinsDepositorInsertTagRFP-CohI
UseTagsStrepIIExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP8 (full length))
Plasmid#16361DepositorInserthuman SENP8 full length (SENP8 Human)
UseTagsHisExpressionBacterialMutationhuman SENP8 full length, see Depositor CommentsPromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-Rapamycin sensor
Plasmid#186292PurposeExpression Rapamycin sensor in e coliDepositorInsertcpLumiLuc-Rapamycin sensor
UseTagsHisTagExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-STING(pLxIS)
Plasmid#221333PurposeOverexpression of STING CTT in bacteriaDepositorInsertSTING CTT (Sting1 Mouse)
UseTags6xHis-4xFKBPExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TGFα-GSY-SPM
Plasmid#163615PurposeExpresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.DepositorInsertTGFa-GSY-SPM (TGFA Synthetic, Human)
UseTagsC-tag and His6ExpressionBacterialMutationPromoterT7Available sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT3
Plasmid#53559Purposebacterial expression of IFIT3DepositorInsertIFIT3 (IFIT3 Human)
UseTags6xHIS and TEVExpressionBacterialMutationPromoterT7Available sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP5 (catalytic domain)
Plasmid#16358DepositorInserthuman SENP5 catalytic domain (SENP5 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 567 to 755 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT2
Plasmid#53558Purposebacterial expression of IFIT2DepositorInsertIFIT2 (IFIT2 Human)
UseTags6xHIS and TEVExpressionBacterialMutationPromoterT7Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a SV40 ST full length
Plasmid#132636Purposeexpresses SV40 ST full length in E. coliDepositorInsertSV40 small t antigen
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-his-Gluc-iLID
Plasmid#172096PurposeExpresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteriaDepositorInsertGaussia Luciferase - iLID
UseTagsExpressionBacterialMutationGluc Mutations - M43L, M110LPromoterT7Available sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ClfB_N2N3-HIS-ybbr
Plasmid#101717PurposeClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertClfB_N2N3
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP3(307-574)
Plasmid#71481PurposeEncodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 3 (SENP3 Human)
UseTagsHexahistidineExpressionBacterialMutationDeleted amino acids 1-306 (catalytic domain only)PromoterT7Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hDcp2 E147Q, E148Q
Plasmid#72215Purposebacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorInsertDcp2 (DCP2 Human)
UseTagsHisExpressionBacterialMutationE147Q, E148QPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160-GCN4
Plasmid#74608PurposeExpresses artificially dimerized Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
UseTags6x His, GCN4, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
WT N-His Ana GvpC in pET28a
Plasmid#153294PurposeExpresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coliDepositorInsertGas vesicle protein C (N-terminal his-tagged)
UseTagsHis-tagExpressionBacterialMutationAdded a hexahistidine tag after the methionine at…PromoterT7Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only