We narrowed to 13,950 results for: UNG

1. pC0090

   Plasmid

   #181954

   Purpose

   Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   7xTOPFlash
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0091

   Plasmid

   #181955

   Purpose

   Beta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/Cluc

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   7xFOP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-MP2

   Plasmid

   #36097

   Purpose

   3rd generation lentiviral plasmid

   Depositor

   Pantelis Tsoulfas

   Article

   Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-mCherry-CDK4KTR

   Plasmid

   #126680

   Purpose

   Fluorescent reporter for CDK4/6 activity

   Depositor

   Hee Won Yang

   Article

   Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

   Insert

   RB cterm
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Frb-EGFP-HOTag6

   Plasmid

   #106919

   Purpose

   Express Frb-EGFP-HOTag6 fusion in mammalian cells.

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   Frb-EGFP-HOTag6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FKBP-EGFP-HOTag3

   Plasmid

   #106924

   Purpose

   Expresses FKBP-EGFP-HOTag3 in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   FKBP-EGFP-HOTag3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC0042-PguCas13b crRNA backbone

   Plasmid

   #103853

   Purpose

   For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-PguCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKAd5f11p-EPG

   Plasmid

   #122697

   Purpose

   To generate E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and an EF1a promoter-controlled transgene.

   Depositor

   Zhuo-Zhuang Lu

   Article

   Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

   Insert

   GFP
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF_dCas9

   Plasmid

   #68416

   Purpose

   Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   human EF1[alpha]

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. TALED_Left-ND1-1397C-AD

    Plasmid

    #183892

    Purpose

    Expresses TALED_Left-ND1-1397C-AD in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TALED_Right-ND1-1397N

    Plasmid

    #183898

    Purpose

    Expresses TALED_Right-ND1-1397N in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. LZF43: Cre-on CAG-CheRiff-CFP (DIO)

    Plasmid

    #104114

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    CheRiff-CFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pM201

    Plasmid

    #173179

    Purpose

    dCas9-24xGCN4

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9-24xGCN4
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. TALED_Left-ND1-AD-E1347A

    Plasmid

    #183894

    Purpose

    Expresses TALED_Left-ND1-AD-E1347A in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. HSA3_His

    Plasmid

    #202765

    Purpose

    human serum albumin design HSA3, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    HSA3
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-BbsI-PITCh

    Plasmid

    #127875

    Purpose

    For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pM370

    Plasmid

    #173178

    Purpose

    (Empty Backbone) gRNA-12xMBS

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    gRNA-12xMBS
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TALED_Right-ND1-AD

    Plasmid

    #183900

    Purpose

    Expresses TALED_Right-ND1-AD in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TALED_Left-ND1-E1347A

    Plasmid

    #183895

    Purpose

    Expresses TALED_Left-ND1-E1347A in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    TALED for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198467

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Use

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pC007 - pRFP constitutive expression

    Plasmid

    #79156

    Purpose

    RFP constitutively expressed

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    RFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pXG237

    Plasmid

    #141281

    Purpose

    ATF4 translational reporter

    Depositor

    Martin Kampmann

    Article

    Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

    Insert

    uORF1/2 of ATF4 followed by mApple
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-Tet

    Plasmid

    #138086

    Purpose

    Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles

    Depositor

    Eva Frickel

    Article

    Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV promoter with heptamerized tet‐operat…

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 5xGAL4-TATA-luciferase

    Plasmid

    #46756

    Purpose

    Luciferase reporter gene for factors with GAL4 DNA binding domains

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    minimal TATA

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWRS_1001

    Plasmid

    #192205

    Purpose

    lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs

    Depositor

    William Sellers

    Article

    Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lenti-entry-puro

    Plasmid

    #85745

    Purpose

    Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pWRS3001_VCR1_WCR3

    Plasmid

    #192206

    Purpose

    VCR1 and WCR3 tracrRNAs cloned into pTWIST AMP high copy backbone

    Depositor

    William Sellers

    Article

    Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

    Insert

    VCR1-WCR3 tracrRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-G-Rac1

    Plasmid

    #180575

    Purpose

    ddGFP sensor of Rac1

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddGFP sensor of Rac1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-R-Rac1

    Plasmid

    #180576

    Purpose

    ddRFP sensor of Rac1

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddRFP sensor of Rac1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXG260

    Plasmid

    #141282

    Purpose

    CMV:EGFP (as control for pXG237)

    Depositor

    Martin Kampmann

    Article

    Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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