Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 23,099 results for: crispr

Showing: 11951 - 12000 of 23099 results

  1. pLKO_NWS.Ollas.S_mCherry-NLS

    Plasmid
    #178279
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    NWS.Ollas.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178279-plasmid-map-sequence-id-353143

    Enlarge

  2. pLKO_HSV.S.V5_mCherry-NLS

    Plasmid
    #178278
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.S.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178278-plasmid-map-sequence-id-353142

    Enlarge

  3. pLKO_HSV.Ollas.V5_mCherry-NLS

    Plasmid
    #178277
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.Ollas.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178277-plasmid-map-sequence-id-353139

    Enlarge

  4. pLKO_HSV.NWS.S_mCherry-NLS

    Plasmid
    #178273
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.NWS.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178273-plasmid-map-sequence-id-353133

    Enlarge

  7. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid
    #140193
    Purpose
    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta), NLS
    Expression
    Mutation
    D832A DNAse deactivated
    Promoter
    PgpdA and PtrpC
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140193-plasmid-map-sequence-id-309648

    Enlarge

  10. PB-PE VQR

    Plasmid
    #162797
    Purpose
    All-in-one prime editor piggyBac transposon, VQR variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    CAG
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    162797-plasmid-map-sequence-id-320036

    Enlarge

  15. pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113689
    Purpose
    SaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113689-plasmid-map-sequence-id-231823

    Enlarge

  16. pLKO_HA.S.VSVg_mCherry-NLS

    Plasmid
    #178269
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HA.S.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178269-plasmid-map-sequence-id-353129

    Enlarge

  17. pLKO_HA.HSV.VSVg_mCherry-NLS

    Plasmid
    #178261
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HA.HSV.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178261-plasmid-map-sequence-id-353016

    Enlarge

  18. pLKO_HA.HSV.S_mCherry-NLS

    Plasmid
    #178260
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HA.HSV.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178260-plasmid-map-sequence-id-353014

    Enlarge

  19. pLKO_C.S.VSVg_mCherry-NLS

    Plasmid
    #178255
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.S.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178255-plasmid-map-sequence-id-353006

    Enlarge

  20. pLKO_C.HSV.VSVg_mCherry-NLS

    Plasmid
    #178246
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HSV.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178246-plasmid-map-sequence-id-352849

    Enlarge

  21. pLKO_C.HSV.S_mCherry-NLS

    Plasmid
    #178245
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HSV.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178245-plasmid-map-sequence-id-352848

    Enlarge

  22. pLKO_C.HA.VSVg_mCherry-NLS

    Plasmid
    #178241
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HA.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178241-plasmid-map-sequence-id-352841

    Enlarge

  23. pLKO_C.HA.S_mCherry-NLS

    Plasmid
    #178240
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HA.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178240-plasmid-map-sequence-id-352840

    Enlarge

  24. pLKO_C.HA.HSV_mCherry-NLS

    Plasmid
    #178237
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HA.HSV and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178237-plasmid-map-sequence-id-352835

    Enlarge

  25. pLKO_AU1.HSV.VSVg_mCherry-NLS

    Plasmid
    #178225
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HSV.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178225-plasmid-map-sequence-id-352775

    Enlarge

  26. pTE3328

    Plasmid
    #107539
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    As crRNA
    hAsCpf1(RR mutant)
    Use
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K607R
    Promoter
    CMV and human U6
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107539-plasmid-map-sequence-id-207236

    Enlarge

  28. pLKO_AU1.HSV.Ollas_mCherry-NLS

    Plasmid
    #178223
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HSV.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178223-plasmid-map-sequence-id-352772

    Enlarge

  29. pLKO_AU1.HA.V5_mCherry-NLS

    Plasmid
    #178221
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178221-plasmid-map-sequence-id-352765

    Enlarge

  30. pLKO_AU1.HA.Ollas_mCherry-NLS

    Plasmid
    #178218
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178218-plasmid-map-sequence-id-352762

    Enlarge

  31. pLKO_AU1.C.V5_mCherry-NLS

    Plasmid
    #178215
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178215-plasmid-map-sequence-id-352759

    Enlarge

  32. pLKO_AU1.C.Ollas_mCherry-NLS

    Plasmid
    #178212
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178212-plasmid-map-sequence-id-352756

    Enlarge

  34. pCAT526

    Plasmid
    #180513
    Purpose
    Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv1
    PlmCasX-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180513-plasmid-map-sequence-id-359728

    Enlarge

  35. pLKO_HSV.Ollas.S_mCherry-NLS

    Plasmid
    #178276
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.Ollas.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178276-plasmid-map-sequence-id-353137

    Enlarge

  36. pLKO_HA.HSV.Ollas_mCherry-NLS

    Plasmid
    #178259
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HA.HSV.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178259-plasmid-map-sequence-id-353013

    Enlarge

  37. pLKO_HA.HSV.NWS_mCherry-NLS

    Plasmid
    #178258
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HA.HSV.NWS and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178258-plasmid-map-sequence-id-353011

    Enlarge

  38. p426_Cas9_gRNA-R-ARS607c

    Plasmid
    #87409
    Purpose
    p426_Cas9_gRNA-ARS607c without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS607c sequence CTATTTTTGCTTTCTGCACA in yeast chromosome 6.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS607c
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87409-plasmid-map-sequence-id-174884

    Enlarge

  39. pTE3329

    Plasmid
    #107538
    Purpose
    Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Lb crRNA
    hLbCpf1(RR mutant)
    Use
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    G532R, K595R
    Promoter
    CMV and human U6
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107538-plasmid-map-sequence-id-207235

    Enlarge

  40. pTE3327

    Plasmid
    #107535
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    As crRNA
    hAsCpf1(RVR mutant)
    Use
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K548V, N552R
    Promoter
    CMV and human U6
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107535-plasmid-map-sequence-id-207331

    Enlarge

  42. pSc2

    Plasmid
    #80437
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Available Since
    Sept. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    80437-plasmid-map-sequence-id-151450

    Enlarge

  45. pTE3334

    Plasmid
    #107537
    Purpose
    Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Mb crRNA
    hMbCpf1(RVR mutant)
    Use
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N576R, K582V, N586R
    Promoter
    CMV and human U6
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107537-plasmid-map-sequence-id-207346

    Enlarge

  46. pTE3330

    Plasmid
    #107534
    Purpose
    Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Lb crRNA
    hLbCpf1(RVR mutant)
    Use
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    G532R, K538V, Y542R
    Promoter
    CMV and human U6
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107534-plasmid-map-sequence-id-207314

    Enlarge

  47. pVD1 Multiplexing Edit En-1 (GB2242)

    Plasmid
    #160564
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the position [5_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    Multiplexing Edit (En-1)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160564-plasmid-map-sequence-id-319675

    Enlarge

  49. AcrIIC1X* (AcrIIC1X-mCherry chimera)

    Plasmid
    #128114
    Purpose
    Expresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9
    Depositor
    Dominik Niopek
    Article
    Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038
    Insert
    AcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    128114-plasmid-map-sequence-id-249192

    Enlarge

  50. pTE3336

    Plasmid
    #107541
    Purpose
    Expresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Mb crRNA
    hMbCpf1(RR mutant)
    Use
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N576R, K637R
    Promoter
    CMV and human U6
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107541-plasmid-map-sequence-id-207245

    Enlarge

Showing: 11951 - 12000 of 23099 results