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pAG32-L-IFP-F[2] (clon 3)

Plasmid

#52900

Purpose

Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Vector for homologous recombination

Promoter

TEF promoter

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pMaCTag-P15

Plasmid

#120026

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-11

Plasmid

#119990

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE150

Plasmid

#118500

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform III

Plasmid

#120319

Purpose

Septin 11 isoform III mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pgRNAkan-glpR

Plasmid

#169873

Purpose

Single guide RNA plasmid for Cas9 targeting glpR in P. putida

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

sgRNA towards glpR in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBBK83 pHomL-RPL18Bp-ERG6-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179067

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Scarlet(rfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ERG6-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK82 pHomL-RPL18Bp-Scarlet(rfp)-Link-ATG8-SSA1t-HomR (AmpR)

Plasmid

#179066

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-ATG8, a marker for the autophagosome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-ATG8

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK76 pHomL-RPL18Bp-PIL1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179060

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Scarlet(rfp), a marker for the eisosomes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PIL1-Link-Scarlet(rfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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3XFlag-pLPC

Plasmid

#73560

Purpose

vector encoding 3 Flag tags

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

3X-Flag

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pAJT430

Plasmid

#202291

Purpose

RPU integration plasmid (LPattB7)

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

J23101-RiboJ00-B0034-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {ChETA}on-WPRE

Plasmid

#111386

Purpose

AAV expression of ChETA (hChR2 with E123T/H134R mutations) fused to HA tag driven by hSynapsin promoter in Cre-ON manner for optogenetic activation

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Insert

ChETA

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

humanized ChR2, E123T/H134R

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRL8

Plasmid

#24980

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2010 Jun;7(6):451-3. doi: 1

Insert

Cbr-unc-119

Use

Multisite gateway

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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Sm-Myocardin Omni-pShuttle

Plasmid

#122122

Purpose

Expression of the smooth muscle version of myocardin

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Myocardin (Myocd Mouse)

Tags

Omni

Expression

Mammalian

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)

Plasmid

#70211

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deCFP

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP97

Plasmid

#83551

Purpose

Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

CYC1 promoter-yEGFP

Expression

Yeast

Promoter

CYC1

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Pr-deGFP-MGapt

Plasmid

#67734

Purpose

OR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCLC-mEos2

Plasmid

#38010

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

mEos2

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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ptdEos-CLC

Plasmid

#38009

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Inserts

Clathrin, light polypeptide (LCa) (Clta Mouse)
Eos FP, tandem dimer

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAJT254-litR

Plasmid

#202308

Purpose

litR repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

litR

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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