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pMGF173

Plasmid

#96999

Purpose

NLS-GFP-lacZ leu1 insertion vector

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

lacZ

Tags

NLS-GFP

Expression

Yeast

Promoter

Pnmt1

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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Pr2-T7RNAP

Plasmid

#67739

Purpose

OR2-OR1-P2 promoter expressing T7 RNAP

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-T7RNAP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr2

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pattB-3xFlag-Loqs-PD

Plasmid

#41110

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Loquacious-PD (loqs Fly)

Tags

3xFlag

Expression

Insect

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
pNP1 OR sfGFP

Plasmid

#107364

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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p3039 pVL E6-AP

Plasmid

#8646

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Insect

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pBS mouse IRS4 promoter

Plasmid

#11445

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-4 promoter (Irs4 Mouse)

Expression

Bacterial

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pNL1 Ecarin

Plasmid

#106289

Purpose

Expresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Ecarin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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907K

Plasmid

#160236

Purpose

FlightN-PipR-VP16-Sv40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

pristinamycin repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAP1680

Plasmid

#137088

Purpose

Plasmid for PCR-generated donor DNA for rapid tagging of proteins with tagRFP

Depositor

Geraldine Seydoux

Article

Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

Insert

tagRFP

Use

Pcr template for donor dna

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGBKT7-PRP5

Plasmid

#87592

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp5

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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Cav1-miniSOG

Plasmid

#67667

Purpose

Mammalian expression vector driving dog Caveolin1-minSOG

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

Dog Cav1-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 Y292C

Plasmid

#87291

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 Y292C

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

Y292C

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-56

Plasmid

#92260

Purpose

Plasmid for expression of HA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

Depositor

Marina Chekulaeva, Roy Parker

Article

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 (gw Fly)

Tags

HA

Expression

Insect

Promoter

Ac5

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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Pr-delRBS-deGFP

Plasmid

#67744

Purpose

Pr promoter expressing a deGFP that is missing a ribosomal binding site

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

deGFP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pMT mir-1-1 reporter cel-mir-40

Plasmid

#46739

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-40

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Promoter

Metallothionein (MT)

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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MAGE-A2Pro pGL4basic

Plasmid

#78137

Purpose

luciferase reporter plasmid

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MAGE-A2 (MAGEA2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains -549/+332 of promoter

Promoter

MAGE-A2

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only
MAGE-A1Pro pGL4basic

Plasmid

#78136

Purpose

luciferase reporter plasmid

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MAGE-A1 (MAGEA1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains -82/+116 of promoter

Promoter

MAGE-A1

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDD401

Plasmid

#91828

Purpose

Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Pmyo-2::GFP

Use

CRISPR

Expression

Worm

Mutation

Nucleotide 489 of the myo-2 promoter was changed …

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-R1ACR_653-EYFP

Plasmid

#111079

Purpose

Anion channelrhodopsin from Rhodomonas sp. for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin R1ACR_653

Tags

EYFP

Expression

Mammalian

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pNP1 AND gate sfGFP

Plasmid

#107361

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 AND input A and pNP1 AND input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

AND gate + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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