We narrowed to 23,163 results for: CRISPR

1. pcDNA3-P1-LoxP-Neo-N-miRFP670-C

   Plasmid

   #159434

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Inserts

   Neomycin
   miRFP670
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Promoter

   SV40

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

   Plasmid

   #105039

   Purpose

   Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Tsc2 (Tsc2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA3_ASCL1

   Plasmid

   #64131

   Purpose

   Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1_ASCL3 (ASCL1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPN062

   Plasmid

   #91595

   Purpose

   Express sgRNA targeting human DFNA5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DFNA5 (GSDME Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPN063

   Plasmid

   #91596

   Purpose

   Express sgRNA targeting human DFNA5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DFNA5 (GSDME Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLmoCascade-IL1RNcrRNA

   Plasmid

   #126487

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   Lmo IL1RN cr03
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY005 (pcDNA3.1-hLb3Cpf1)

   Plasmid

   #69977

   Purpose

   Expresses humanized Lb3Cpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hLb3Cpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pJZC42

   Plasmid

   #66564

   Purpose

   sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 1XPP7
   PCP-VP64 IRES mCherry
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDECKO_mCherry_CD19.4

   Plasmid

   #140101

   Purpose

   CRISPR-Cas9 Knock down

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1375_pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-CB-SACas9-HA-OLLAS-spA

    Plasmid

    #109311

    Purpose

    Plasmid for AAV SaCas9 Mammalian Expression with a gRNA against Ldlr

    Depositor

    William Lagor

    Article

    Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

    Insert

    Ldlr gRNA
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC147-pCR8-dCas9VP64

    Plasmid

    #48219

    Purpose

    dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP64 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP64

    Mutation

    D10A;H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVC297 VEGF Site#1

    Plasmid

    #47505

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 1 (VEGFA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVC228 VEGF Site#3

    Plasmid

    #47507

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 3 (VEGFA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

    Plasmid

    #105040

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102890

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHS1068

    Plasmid

    #205962

    Purpose

    NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH proteins and CRISPR array
    

    Expression

    Bacterial

    Promoter

    Lac (proteins), pJ23119 (crRNA)

    Available Since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

    Plasmid

    #132560

    Purpose

    Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Inserts

    ABEmax
    sgRNA, with Tetraloop replaced by com aptamer
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgRNA1_IL1RN

    Plasmid

    #64140

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PAX6 sgRNA7

    Plasmid

    #68466

    Purpose

    targeting PAX6 gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    sgRNA targeting PAX6 (PAX6 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

    Plasmid

    #140205

    Purpose

    Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    mCherry
    sgRNA E1
    

    Use

    CRISPR and Synthetic Biology; Proof-of-concept te…

    Mutation

    G174D

    Promoter

    PelcA and U3

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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