We narrowed to 13,035 results for: Mpl

1. pU6-DHFR

   Plasmid

   #80329

   Purpose

   Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Insert

   DHFR-TSc3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   DHFR-TS 5' UTR

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-eCas9-sgNT

   Plasmid

   #140238

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-MR1

   Plasmid

   #178603

   Purpose

   Mammalian expression of myc-tagged MR1

   Depositor

   Erik Procko

   Article

   McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

   Insert

   MR1 (MR1 Human)
   

   Use

   Tags

   Extracellular c-myc epitope ta and Signal peptide…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pISH-Gap43

   Plasmid

   #74336

   Purpose

   Use to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.

   Depositor

   Peter Mombaerts

   Article

   Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

   Insert

   growth associated protein 43 (Gap43 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK1290-AAV-dSaCas9

   Plasmid

   #223139

   Purpose

   AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEYFP-Sec24D

   Plasmid

   #66614

   Purpose

   YFP fusion of Sec24D

   Depositor

   David Stephens

   Article

   Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

   Insert

   Sec24D (SEC24D Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pLenti6.3_CRKL-Y207F-V5

   Plasmid

   #196016

   Purpose

   Expresses CRKL Y207F, V5 tagged

   Depositor

   William Hahn

   Article

   Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

   Insert

   CRKL
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

   Plasmid

   #83947

   Purpose

   gRNA cassette-carrying vector with natMX marker

   Depositor

   Irina Borodina

   Article

   Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

   Insert

   gRNA targeting ADE2 gene
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHLP700

   Plasmid

   #203742

   Purpose

   C-terminal tagging of target proteins with AID*/3xHA degron

   Depositor

   Mark Hall

   Article

   Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

   Insert

   IAA17 auxin binding domain codons 71-114
   

   Use

   Pcr amplification template for gene tagging

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHLP701

    Plasmid

    #203743

    Purpose

    C-terminal tagging of target proteins with 3xV5-IAA17 degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xV5

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAtC

    Plasmid

    #78098

    Purpose

    Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance

    Depositor

    Jin-Soo Kim, Sang-Gyu Kim

    Article

    Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

    Plasmid

    #159803

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pscALPS gag-gfp/vpx

    Plasmid

    #115808

    Purpose

    SFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpx

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    gag-gfp
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICH47802-p35S::tdTomato-ER::tNOS

    Plasmid

    #160220

    Purpose

    tdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverse

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    p2x35S::TdTomato-ER::tNOS
    

    Use

    Synthetic Biology; Moclo toolkit level 1 acceptor…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-NavMs

    Plasmid

    #100004

    Purpose

    Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP

    Depositor

    Paul DeCaen

    Article

    Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10

    Insert

    His-NavMs, Voltage gated sodium channel
    

    Use

    Tags

    Hexa-His tag followed by a thrombin cleavage site

    Expression

    Mammalian

    Mutation

    C-terminus from a codon-optimized synthetic gene,…

    Promoter

    CMV

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-SAG1-DHFR

    Plasmid

    #80322

    Purpose

    Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    SAG1 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-Hygromycin-TTF-1

    Plasmid

    #119173

    Purpose

    Retroviral mammalian expression vector containing wt-human TTF-1 cDNA

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac1-Ls-AChBP

    Plasmid

    #63126

    Purpose

    Expression of Lymnaea stagnalis AChBP

    Depositor

    Titia Sixma

    Article

    Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

    Insert

    AChBP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. [pSK729] pRK5 HA-FLCN(R164A)

    Plasmid

    #136147

    Purpose

    mammalian expression of FLCN

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Cell. 2019 Nov 27;179(6):1319-1329.e8. d

    Insert

    FLCN (FLCN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R164A

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-ATXN1-2Q

    Plasmid

    #33239

    Depositor

    Huda Zoghbi

    Article

    Cummings et al Nat Genet. 1998 Jun;19(2):148-54.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    insert contains 2Q (2 glutamines) and there isno …

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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