We narrowed to 62,128 results for: cloning

Showing: 4801 - 4820 of 62128 results

Results per page:

Sort results by:

pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

Plasmid

#176268

Purpose

Expression of dCas12f-VPR-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

dCas12f

Use

Lentiviral

Tags

HA-VPR-mCherry

Expression

Mammalian

Mutation

D326A, D510A

Promoter

PGK

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166013

Purpose

mammalian expression of MERS-CoV soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

MERS-CoV S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30a-Spike-E

Plasmid

#165096

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MSCV-BCL6-t2A-BCL2

Plasmid

#135305

Purpose

Overexpression of multiple human genes (BCL2 and BCL6)

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

BCL6 (BCL6 Human)
BCL2 (BCL2 Human)

Use

Retroviral

Promoter

LTR

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Human J-chain

Plasmid

#158215

Purpose

Express the human J-chain

Depositor

Junyu Xiao

Article

Li et al Science. 2020 Feb 28;367(6481):1014-1017

Insert

JCHAIN (JCHAIN Human)

Tags

8×his

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RKS-Fzd7/8 subtype NGS Wnt

Plasmid

#159628

Purpose

Express Fzd7/8 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7/8 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV-EF1amCherry-2A-OsTIR1-W

Plasmid

#159296

Purpose

Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

OsTIR

Use

Lentiviral

Expression

Mammalian

Promoter

human EF1a promoter

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-His-SCov2-nsp7

Plasmid

#154757

Purpose

Expression of SARS-CoV-2 nsp7

Depositor

Patrick Cramer

Article

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
iOn-CAG∞RFP

Plasmid

#154014

Purpose

Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

mRFP1

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO-GAlpha15-RLuc8

Plasmid

#140984

Purpose

Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlpha15-RLuc8 (GNA15 Human)

Use

Luciferase

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-GfaABC1D-GRAB_Ado1.0

Plasmid

#153288

Purpose

Express the adenosine sensor GRAB_Ado1.0 in astrocytes

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

GfaABC1D

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC-AdEvolve-DEST

Plasmid

#131692

Purpose

A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Type

Empty backbone

Use

Adenoviral

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PL-5LTR-RGR(DMD#1)-AmCyan-A

Plasmid

#138482

Purpose

Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Ribozyme-flanked gRNA and AmCyan

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRm43GW

Plasmid

#133748

Purpose

Three-fragment Multisite Gateway vector construction for red fluorescent seed selection in Arabidopsis thaliana

Depositor

Niko Geldner

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFG7m34GW

Plasmid

#133747

Purpose

Three-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thaliana

Depositor

Niko Geldner

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
DR5rev::erRFP

Plasmid

#61011

Purpose

Auxin signalling reporter (DR5rev) driving RFP (ER-targeted)

Depositor

Alexis Maizel

Article

Marin et al Plant Cell. 2010 Apr;22(4):1104-17. doi:

Insert

DR5rev::erRFP:35S_term

Expression

Plant

Promoter

DR5rev::erRFP:35Sterm

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol1-UAS:GtACR2-tdTomato

Plasmid

#124236

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

GtACR2-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mARG1 cassette 3

Plasmid

#134345

Purpose

Mammalian acoustic reporter gene 1 cassette 3 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Article

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 3

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX305_FKBP12F36V-SHOC2

Plasmid

#134522

Purpose

Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

Depositor

Andrew Aguirre

Article

Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Tags

FKBP12F36V-2xHA

Expression

Mammalian

Mutation

Silent mutations made at wobble base position at …

Promoter

hPGK

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET23b-2E2-HA-mEGFP-6xHis

Plasmid

#129594

Purpose

The encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge