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Plasmid#218273PurposeCRISPR KO plasmid for ALDH7A1 in human cell linesDepositorInsertInserted sgRNA targetting ALDH7A1 (ALDH7A1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV hGRN2
Plasmid#213683PurposeExpresses human granulin-2 with an N-terminal twin-Strep-FLAG tagDepositorInserthuman Granulin-2
UseAAVTagstwin-Strep-FLAGExpressionMutationPromotercytomegalovirus enhancer/chicken β-actin promoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEMS2075
Plasmid#158098PurposeHigh Copy number plasmid containing smCBA insert.DepositorInsertsmCBA
UseTagsExpressionMutationPromotersmCBAAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pPBbsr2-NRG1-ScNeo
Plasmid#209911PurposeTo monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertNRG1-ScNeo (NRG1 Human)
UseTagsmNeonGreen and mScarletExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-HBEGF-mNeonGreen
Plasmid#209912PurposeTo monitor the expression of HB-EGF, the plasmid encodes a recombinant HB-EGF fused intracellularly to the mNeonGreen fluorescent proteinDepositorInsertHBEGF-mNeonGreen (HBEGF Human)
UseLentiviralTagsmNeonGreenExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-EREG-mNeonGreen
Plasmid#209914PurposeTo monitor the expression of Epiregulin, the plasmid encodes a recombinant Epiregulin fused intracellularly to the mNeonGreen fluorescent proteinDepositorInsertEREG-mNeonGreen (EREG Human)
UseLentiviralTagsmNeonGreenExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbleo-TGFa-ScNeo
Plasmid#209904PurposeTo monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertTGFa-ScNeo (TGFA Human)
UseTagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-NRG1-ScNeo
Plasmid#209906PurposeTo monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertNRG1-ScNeo (NRG1 Human)
UseTagsmNeonGreen and mScarletExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-TGFa-ScNeo
Plasmid#209908PurposeTo monitor the status of TGFα, the plasmid encodes a recombinant TGFα, fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertTGFa-ScNeo (TGFA Human)
UseTagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
p40651-EFSNS-FMR1_e1ΔCGG-24xms2
Plasmid#222969PurposeMS2 mRNA reporter with FMR1 5' UTR (CGG repeats removed) and exon 1 sequenceDepositorInsertFMR1-ΔCGG 5' UTR and exon1
UseTagsExpressionMammalianMutation31 CGG repeats deleted from 5' UTRPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1-49535-sgFmr1_CGG5-2
Plasmid#222964PurposeTargeting FMR1 5' UTR for CGG deletionDepositorInsertFMR1 sgRNA (FMR1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-L1-SNAP
Plasmid#223577PurposeLentiviral expression of human PD-L1 with SNAP tag in mammalian cellsDepositorInsertPD-L1 (CD274 Human)
UseLentiviral; mammalian expressionTagsSNAPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-SNAP-PD-1 I126A
Plasmid#223573PurposeLentiviral expression of human PD-1 (I126A) with SNAP tag in mammalian cellsDepositorInsertPD-1 (PDCD1 Human)
UseLentiviral; mammalian expressionTagsSNAPExpressionMutationI126APromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CLIP-PD-L1
Plasmid#223571PurposeLentiviral expression of human PD-L1 with CLIP tag in mammalian cellsDepositorInsertPD-L1 (CD274 Human)
UseLentiviral; mammalian expressionTagsCLIPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1A_Hygro_P2A_RUNX1_R118G_Barcode
Plasmid#170970PurposeOverexpression of RUNX1 R118G mutant protein.DepositorInsertRUNX1 (RUNX1 Human)
UseLentiviralTagsExpressionMammalianMutationR118GPromoterEf1AAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1A_Hygro_P2A_RUNX1_N119I_Barcode
Plasmid#170971PurposeOverexpression of RUNX1 N119I mutant protein.DepositorInsertRUNX1 (RUNX1 Human)
UseLentiviralTagsExpressionMammalianMutationN119IPromoterEf1AAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1A_Hygro_P2A_RUNX1_A120D_Barcode
Plasmid#170972PurposeOverexpression of RUNX1 A120D mutant protein.DepositorInsertRUNX1 (RUNX1 Human)
UseLentiviralTagsExpressionMammalianMutationA120DPromoterEf1AAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1A_Hygro_P2A_RUNX1_T121I_Barcode
Plasmid#170973PurposeOverexpression of RUNX1 T121I mutant protein.DepositorInsertRUNX1 (RUNX1 Human)
UseLentiviralTagsExpressionMammalianMutationT121IPromoterEf1AAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1A_Hygro_P2A_RUNX1_T101I_Barcode
Plasmid#170956PurposeOverexpression of RUNX1 T101I mutant protein.DepositorInsertRUNX1 (RUNX1 Human)
UseLentiviralTagsExpressionMammalianMutationT101IPromoterEf1AAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only