We narrowed to 11,735 results for: pcdna

1. pcDNA3-JNKsub(TA)DD-YPet-NES

   Plasmid

   #84634

   Purpose

   Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   JNKsub(TA)DD-YPet-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nano-lantern-H2B/pcDNA3

   Plasmid

   #51971

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   human H2B

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-4

   Plasmid

   #112839

   Purpose

   transient/stable overexpression

   Depositor

   Tomasz Prószyński

   Article

   Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

   Insert

   Grb2 (Grb2 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SH3 (N-term) domain of Grb2

   Promoter

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 merlin 308-590

   Plasmid

   #11627

   Depositor

   Vijaya Ramesh

   Article

   Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

   Insert

   merlin (NF2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Carboxy-terminal half of merlin isoform 2.

   Promoter

   Available since

   May 3, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-HA-BHLHE40(S243A/E248A)

   Plasmid

   #110155

   Purpose

   Expression of human BHLHE40 (S243A/E248A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

   Insert

   BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S243A/E248A

   Promoter

   CMV

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.2/V5 mmu-mir-138-1

   Plasmid

   #26328

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   mmu-mir-138-1 (Mir138-1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5-MIS12-LAP-BUB1

   Plasmid

   #115904

   Purpose

   Used for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.

   Depositor

   Geert Kops

   Article

   Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

   Insert

   MIS12-BUB1 (MIS12 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   BUB1 fused to MIS12

   Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.3 CAS9 IRES-Dsred

   Plasmid

   #129444

   Purpose

   expression of FnCas9 and DsRed

   Depositor

   Oskar Laur

   Insert

   FnCas9 IRES-Dsred
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-NES-YFP-CCTd

   Plasmid

   #184326

   Purpose

   Mammalian expression of the cytosolic localized YFP-CCTd(peptide of Cav1.3)

   Depositor

   Xiaodong Liu

   Article

   Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

   Insert

   CCTd (CACNA1D Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-NLS-YFP-CCTd

    Plasmid

    #184325

    Purpose

    Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.3)

    Depositor

    Xiaodong Liu

    Article

    Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

    Insert

    CCTd (CACNA1D Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA 3.1 SIRT1-deltaExon2

    Plasmid

    #105671

    Purpose

    Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

    Plasmid

    #80272

    Purpose

    mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of multi-phospho degron (amino acids 282…

    Promoter

    CMV

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.1(-) + SLNCR1 _701-902

    Plasmid

    #86821

    Purpose

    mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3-HA-human MYCN-V421D

    Plasmid

    #74166

    Purpose

    Mammalian expression of human MycN-VD mutant

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MycN (MYCN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    V421D

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-FLAG-HA-CAD S1900A

    Plasmid

    #46240

    Purpose

    FLAG-HA-CAD with S1900 mutated to alanine

    Depositor

    Brendan Manning

    Article

    Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

    Insert

    carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Serine 1900 changed to Alanine (S1900A)

    Promoter

    CMV

    Available since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.2/V5 hsa-mir-483

    Plasmid

    #26315

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-483 (MIR483 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

17. Nano-lantern-peroxisome/pcDNA3

    Plasmid

    #51974

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to peroxisomes

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    SKL

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pCDNA3-Flag-SPRTN-Y117C

    Plasmid

    #110216

    Purpose

    Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

    Depositor

    Kristijan Ramadan, Ivana Marinovic-Terzic

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y117C, P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1+_LN-HA-AGO2-5xE

    Plasmid

    #92011

    Purpose

    Expresses Lambda-N-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Mutation

    S824E, S828E, T830E, S831E, S834E (5xE)

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 Flag MFF iso4 WT

    Plasmid

    #74390

    Purpose

    Flag tagged human MFF isoform 4

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only