We narrowed to 13,291 results for: cDNA

1. cacna2d1 R241A D491A HA pcDNA3

   Plasmid

   #206109

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding

   Depositor

   Annette Dolphin

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R241A D491A

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.1(-) mouse Egr4

   Plasmid

   #108096

   Purpose

   Expresses mouse Egr4 in mammalian cells

   Depositor

   Peter Johnson

   Article

   Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

   Insert

   Egr4 (Egr4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. OeNL(Ca2+)_18μ-pcDNA3

   Plasmid

   #111928

   Purpose

   Orange color bright luminescent calcium indicator with intermediate affinity

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   OeNL(Ca2+)_18μ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E67D, E104D, D133E at CaM

   Promoter

   CMV

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG

   Plasmid

   #127322

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO HIS DNAJB8

   Plasmid

   #19558

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)Gαq-124-NLuc

   Plasmid

   #207153

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAQ (GNAQ Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position F(124)E

   Promoter

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

   Plasmid

   #207160

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNG2 (GNG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N-terminally tagged with LSS-mKATE2

   Promoter

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3 AU1 mTOR S2035W

   Plasmid

   #15678

   Depositor

   John Lawrence

   Article

   McMahon et al Mol Cell Biol. 2002 Nov . 22(21):7428-38

   Insert

   mTOR (Mtor Rat)
   

   Use

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   S2035W

   Promoter

   Available Since

   Sept. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5-FRT/TO-Hygro-3xFLAG

   Plasmid

   #185359

   Purpose

   Vector backbone for mammalian expression of proteins with an N-terminal 3xFLAG tag.

   Depositor

   Ayaz Najafov

   Insert

   3xFLAG
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 3495 pcDNA3 Bid p15

    Plasmid

    #8780

    Depositor

    Stanley Korsmeyer

    Article

    Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

    Insert

    Bid p15 (Bid Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

11. EGFP Rab11a WT pcDNA3.1 Zeo (+)

    Plasmid

    #206079

    Purpose

    Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

    Depositor

    Annette Dolphin

    Article

    Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-FLAG-mTET2 (N500)

    Plasmid

    #89897

    Purpose

    Expresses mouse TET2 N500 truncation in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    TET2 (Tet2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-term 500 truncation of mouse TET2 (contains fir…

    Promoter

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1/Hygro-Mfn2-ActA

    Plasmid

    #191981

    Purpose

    Expression of Mfn2 tagged with mitochondrion localization signal (ActA)

    Depositor

    Luca Scorrano

    Insert

    Mfn2-ActA
    

    Use

    Tags

    ActA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 PBD HK-AA (Nigg AH13)

    Plasmid

    #41161

    Depositor

    Erich Nigg

    Article

    Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

    Insert

    PBD HK-AA (PLK1 Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    Contains Plk1-PBD only (aa 326–603); HK-AA (H538A…

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5-FRT-T0-Flag-HROB

    Plasmid

    #135298

    Purpose

    Expresses Flag-tagged HROB in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Hustedt et al Genes Dev. 2019 Oct 1;33(19-20):1397-141

    Insert

    HROB (HROB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. EphA2 430-976 pcDNA3.1

    Plasmid

    #102738

    Purpose

    Expression of c terminal EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

    Insert

    EphA2 N terminal (430-976aa) (Epha2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+) neo with Smad5 CDS

    Plasmid

    #61800

    Purpose

    pcDNA3.1(+) with full length mouse Smad5 CDS

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    Smad5 CDS (Smad5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

18. 3578 pcDNA3 Bad S112A

    Plasmid

    #8797

    Depositor

    Stanley Korsmeyer

    Article

    Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

    Insert

    Bad (Bad Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S112A

    Promoter

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

19. CeNL(Ca2+)_19μ-pcDNA3

    Plasmid

    #111927

    Purpose

    Cyan color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_19μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, F92W, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-puro-eGFP-HEC1-9A

    Plasmid

    #114023

    Purpose

    Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - 9A (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Hec1 - 9A

    Promoter

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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