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pMD2.G-K47Q/R354Q

Plasmid

#207323

Purpose

VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

Depositor

Feng Zhang

Publication

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

VSV-G (VSIVgp4 Vesicular Stomatitis Virus)

Expression

Mammalian

Mutation

K47Q/R354Q

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGL410_Ins2500

Plasmid

#49058

Purpose

Expresses luciferase under control of mouse insulin 2 gene promoter

Depositor

Kevin Ferreri

Publication

Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mouse Ins2 promoter (Ins2 Mouse)

Expression

Mammalian

Promoter

Ins2 promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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XRE-pNL1.3[secNLuc]

Plasmid

#182295

Purpose

Secreted Luciferase reporter for AhR activity

Depositor

Severine Degrelle, Thierry Fournier

Publication

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Xenobiotic Response Element (CYP1A1 Human)

Tags

secNluc

Expression

Mammalian

Promoter

XRE

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Tagged-IRES

Plasmid

#35570

Depositor

Marian Waterman

Publication

Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lymphoid Enhancer Factor 1 5'UTR (LEF1 Human)

Use

Luciferase

Tags

Firefly Luciferase and MS2 Stem Loop

Expression

Mammalian

Promoter

SV40

Available since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-CA-FLEX-RG

Plasmid

#38043

Depositor

Naoshige Uchida

Publication

Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

Insert

Rabies virus glycoprotein

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CA

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMUH_unc84_2XGFP

Plasmid

#46023

Depositor

Sean Eddy

Publication

Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

Insert

UNC-84 (unc-84 )

Use

10xuas-phi31-expression vector

Tags

2XGFP

Mutation

Two amino acid mutations (F279L and L500P) compar…

Promoter

10XUAS

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV2.5-THP-dsRed2

Plasmid

#80335

Purpose

Fluorescent Reporter for Dopaminergic Neuron Differentiation

Depositor

Kwang-Soo Kim

Publication

Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

Insert

rat TH promoter (Th Rat)

Use

AAV

Promoter

rat Tyrosine hydroxylase

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pComb3

Plasmid

#63888

Purpose

For phage display of antibody libraries by fusion to modified geneIII.

Depositor

Carlos Barbas

Publication

Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

Type

Empty backbone

Use

Phage display

Tags

geneIII

Expression

Bacterial

Promoter

lacZ

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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ORF3a-EGFP

Plasmid

#165121

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Publication

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ORF3a (ORF3a codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pETM33_Nsp15

Plasmid

#156481

Purpose

Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Publication

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp15 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHCMV-VSVgSS-pAG-TM

Plasmid

#207318

Purpose

Functional incorporation of protein AG (pAG) fusion protein into envelope of virions

Depositor

Feng Zhang

Publication

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

protein AG

Expression

Mammalian

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRluc-LC3wt

Plasmid

#105002

Purpose

Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Publication

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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145-pGL3ctrl-3' UTR

Plasmid

#28175

Depositor

Michael Kastan

Publication

Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Luciferase flanked by tp53 UTRs (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Note that insert length is based on sum of the 2 …

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pAAV-mCAMK-RFP

Plasmid

#22908

Depositor

Edward Callaway

Publication

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Insert

mouse alpha-calcium/calmodulin-dependent protein kinase II promoter (Camk2a Mouse)

Use

AAV

Expression

Mammalian

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDD315 (mTurquoise2^SEC^2xHA)

Plasmid

#73343

Purpose

mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Publication

Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

Insert

mTurquoise2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

2xHA and C. elegans codon-optimized mTurquoise2

Expression

Worm

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pComb8

Plasmid

#63889

Purpose

For phage display of antibody libraries by fusion to modified geneVIII.

Depositor

Carlos Barbas

Publication

Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

Type

Empty backbone

Use

Phage display

Tags

geneVIII

Expression

Bacterial

Promoter

lacZ

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-CA-FLEX

Plasmid

#38042

Purpose

Cre activates gene of interest, using 4 mutated lox sites

Depositor

Naoshige Uchida

Publication

Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

Type

Empty backbone

Use

AAV and Cre/Lox

Expression

Mammalian

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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10XSTAT92E–luciferase

Plasmid

#37393

Depositor

Norbert Perrimon

Publication

Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

Insert

SOCS36E enhancer fragment (Socs36E Fly)

Use

Luciferase; Jak/stat reporter construct

Expression

Insect

Promoter

Hsp70

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCFJ210 - pDESTR4-R3(ttTi4348, I)

Plasmid

#34863

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi4348 targeting

Use

Worm targeting

Available since

June 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670)

Plasmid

#100061

Purpose

AAV expression of ChR1-VChR1 Chimera fused to mCherry driven by Ef1a promoter

Depositor

Karl Deisseroth

Has service

AAV9

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E122T and E162T

Promoter

EF1a

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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