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pLKO.1 puro GFP siRNA

Plasmid

#12273

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

GFP siRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt11

Plasmid

#35922

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt11 (wnt11f2 Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Snail_pGL2

Plasmid

#31694

Depositor

Paul Wade

Article

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Snail (SNAI1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A

Plasmid

#102585

Purpose

Control lentivector. Coexpression of mTagBFP and gene of interest from SFFV promoter

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-FOXK1

Plasmid

#153132

Purpose

Expression of Flag tagged human FOXK1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXK1 (FOXK1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pACAGW-oChiEFtdTomato-AAV

Plasmid

#32846

Purpose

AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

Depositor

Rory McQuiston

Article

Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

Insert

oChiEF

Use

AAV

Tags

tdTomato

Promoter

CAGG

Available Since

March 19, 2012

Availability

Academic Institutions and Nonprofits only

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pHR-flag-GFP-JAM-B

Plasmid

#205191

Purpose

This vector encodes of a synCAM (JAM-B) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM JAM-B and GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBGSA C5aR1-EGFP

Plasmid

#62612

Purpose

Constitutive expression of C5aR1-EGFP in mammalian cells

Depositor

Heini Miettinen

Article

Suvorova et al Traffic. 2005 Feb;6(2):100-15.

Insert

C5aR1 (C5AR1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

SRalpha

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMK1221

Plasmid

#84220

Purpose

Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, mCherry

Depositor

Martin Kampmann, Jonathan Weissman

Article

Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSico PGK puro

Plasmid

#11586

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-NLuc_SV40:PuroR

Plasmid

#183045

Purpose

Expresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

NanoLuciferase

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-FOXL1

Plasmid

#153134

Purpose

Expression of Flag tagged human FOXL1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXL1 (FOXL1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Vinculin-venus

Plasmid

#27300

Depositor

Martin Schwartz

Article

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

Vinculin (VCL Chicken)

Tags

Venus A206K

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

Plasmid

#134662

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 5 (VAMP5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-hAgo2

Plasmid

#11590

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

Ago2 (AGO2 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only

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pdCas9-J23111

Plasmid

#113149

Purpose

Plasmid constitutively expressing dCas9 protein

Depositor

Chong Zhang

Article

Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

Insert

dCas9

Expression

Bacterial

Mutation

D10A & H840A

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSiMPlk_N + pSiMPlk_C

Plasmid

#134310

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

kanR_gp41-1 N-intein + kanR_gp41-1 C-intein

Expression

Bacterial

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-LNGFR-recipient

Plasmid

#11660

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

LNGFR

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PPARgamma2

Plasmid

#78768

Purpose

To overexpress PPARgamma2 in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPARG (Pparg Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pMpGWB103

Plasmid

#68557

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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