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Showing: 7291 - 7320 of 8645 results

  1. pLyGo-Bs-3

    Plasmid
    #163141
    Purpose
    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PamyL-PamyQ-PcryIIIA-cryIIIAstab49
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163141-plasmid-map-sequence-id-332141

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  2. pLyGo-Bs-2

    Plasmid
    #163140
    Purpose
    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PamyL-PamyQ-PcryIIIA-cryIIIAstab49
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163140-plasmid-map-sequence-id-332139

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  5. pSWD95Y14D

    Plasmid
    #159098
    Purpose
    CckA FRET Sensor with cyclic-di-GMP point mutant
    Depositor
    Seth Childers
    Article
    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do
    Insert
    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
    Expression
    Bacterial
    Mutation
    Mutated Y514 to D514
    Promoter
    Xylose
    Available Since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159098-plasmid-map-sequence-id-310723

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  6. pSWD95D293A

    Plasmid
    #159099
    Purpose
    CckA FRET Sensor with DITE motif mutant
    Depositor
    Seth Childers
    Article
    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do
    Insert
    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
    Expression
    Bacterial
    Mutation
    CckA D293 mutated to A293 and V199I on mRuby3 (P…
    Promoter
    Xylose
    Available Since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159099-plasmid-map-sequence-id-316589

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  11. MTK0_004

    Plasmid
    #123961
    Purpose
    Encodes the Cas9 cROSA26 homology destination with Blasticidin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    cRosa26 destination
    Expression
    Mammalian
    Available Since
    Feb. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    123961-plasmid-map-sequence-id-272155

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  15. MTK0_052

    Plasmid
    #123978
    Purpose
    Encodes the PhiC31 landing pad on hThal5.10-2-hg18 with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PhiC31 LP on hThal5.10-2-hg18 (Hygro::mRuby)
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123978-plasmid-map-sequence-id-251837

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  16. MTK0_054

    Plasmid
    #123980
    Purpose
    Encodes the PhiC31 landing pad on hCLYBL with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PhiC31 LP on hCLYBL (Hygro::mRuby)
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123980-plasmid-map-sequence-id-251849

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  21. MTK6_013

    Plasmid
    #123867
    Purpose
    Encodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    WPRE-Blast (no terminator)
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123867-plasmid-map-sequence-id-249818

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  22. pEPOR2KN0078

    Plasmid
    #117645
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    Expression
    Plant
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    117645-plasmid-map-sequence-id-240245

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  23. pGEM-myr-Citrine-SV40pA-FKF

    Plasmid
    #119865
    Purpose
    Donor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    myr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Tags
    2x myristoylation
    Expression
    Bacterial
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    119865-plasmid-map-sequence-id-239419

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  24. pOCC17

    Plasmid
    #118875
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal tagRFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-tagRFP-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and tagRFP
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118875-plasmid-map-sequence-id-232352

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  25. pOCC16

    Plasmid
    #118870
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal EGFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-EGFP-stop-HindIII cassette
    Tags
    EGFP and HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118870-plasmid-map-sequence-id-232333

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  26. pOCC218

    Plasmid
    #118907
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS10, cleavable with 3C protease and N-terminal YBBR tag
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS10-3C-YBBR-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS10, cleavable with 3C protease, YBBR tag
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118907-plasmid-map-sequence-id-232329

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  27. pOCC18

    Plasmid
    #118868
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal tagRFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-tagRFP-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, tagRFP
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118868-plasmid-map-sequence-id-232324

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  28. pOCC171

    Plasmid
    #118888
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tag
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease; SNAP tag
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118888-plasmid-map-sequence-id-232245

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  29. pOCC202

    Plasmid
    #118880
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    Tags
    mKate2, HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118880-plasmid-map-sequence-id-232243

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  30. pOCC139

    Plasmid
    #118872
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and mGFP
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118872-plasmid-map-sequence-id-232236

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Showing: 7291 - 7320 of 8645 results