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HT114_Fsyn_FAS(Cre off)_QuasAr6b_Citrine

Plasmid

#178825

Purpose

Neuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6b_citrine

Use

Cre/Lox and Lentiviral

Tags

citrine

Expression

Mammalian

Promoter

hSyn

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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3020_pETcon-SARS-CoV-2-RBD_N501Y

Plasmid

#184405

Purpose

yeast surface display of the SARS-CoV-2 Alpha variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Alpha Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

N501Y

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot4-dsRNAres_Q

Plasmid

#147340

Purpose

Insect Expression of DmNot4-dsRNAres

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

DmNot4-dsRNAres (CG31716 Fly)

Expression

Insect

Mutation

7 silent mutations and 6 non silent mutations G20…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHcRed-NPMMLF1nes-C1

Plasmid

#131849

Purpose

Expresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfection

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Tags

HcRed

Expression

Mammalian

Mutation

This is a control deletion contruct of the cytop…

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-IpgD

Plasmid

#183672

Purpose

Inducible Shigella flexneri IpgD for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Expression

Yeast

Promoter

GAL1

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-IpgD(C439S)

Plasmid

#183673

Purpose

Inducible Shigella flexneri IpgD (catalytically inactive) for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Expression

Yeast

Mutation

C439S

Promoter

GAL1

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Arg57Ala

Plasmid

#180759

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Arg57Ala

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Arg57Ala, Leu1…

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Tyr139Ala

Plasmid

#180761

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Tyr139Ala

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Tyr139Ala, Leu…

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)

Plasmid

#166855

Purpose

Expresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

Insert

Atg13[571–700] (ATG13 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K683E, H685E, K686E

Promoter

pCu

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)

Plasmid

#166854

Purpose

Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

Insert

Atg13[571–700] (ATG13 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

F641E, I645E

Promoter

pCu

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)

Plasmid

#166853

Purpose

Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

Insert

Atg13[571–700] (ATG13 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

F641G, I645G

Promoter

pCu

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 SEC66-VAC8-GFP

Plasmid

#166840

Purpose

Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, SEC66 Budding Yeast)

Tags

GFP and SEC66 transmembrane domain

Expression

Yeast

Mutation

SEC66 transmembrane domain

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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UbC-Halo-SFPQY527A

Plasmid

#166947

Purpose

Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Use

Lentiviral

Tags

Halo

Mutation

SFPQ-Y527A

Promoter

UbC

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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T7pr-His6-MBP-TEV-Cas9-BirA*

Plasmid

#159993

Purpose

Bacterial expression of Cas9-BirA*

Depositor

Jacob Corn

Article

Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

Insert

Cas9-BirA*

Tags

6X-His, MBP, 3X-FLAG, NLS

Expression

Bacterial

Mutation

D10A, H840A

Promoter

T7

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-mu24-ORF24-2xStrep

Plasmid

#138453

Purpose

Expresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

mu24-ORF24 (ORF24 MHV68/KSHV)

Tags

Strep

Expression

Mammalian

Mutation

mu24 amino acids 1-191 fused to ORF24 amino acids…

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-BcRF1-ORF24-2xStrep

Plasmid

#138454

Purpose

Expresses BcRF1-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1-ORF24 (BcRF1, ORF24 EBV/KSHV)

Tags

Strep

Expression

Mammalian

Mutation

BcRF1 amino acids 1-178 fused to ORF24 amino acid…

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-UL87-ORF24-2xStrep

Plasmid

#138455

Purpose

Expresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87-ORF24 (UL87, ORF24 HCMV/KSHV)

Tags

Strep

Expression

Mammalian

Mutation

UL87 amino acids 1-248 fused to ORF24 amino acids…

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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EcNGsC-F307Y-pKK223

Plasmid

#131381

Purpose

Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307Y in Gs MutY.

Depositor

Martin Horvath

Article

Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

Insert

EcNGsC MutY chimera F307Y

Expression

Bacterial

Mutation

Fusion of two MutY genes. Residues 1-225 are from…

Promoter

tac

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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EcNGsC-F307A-pKK223

Plasmid

#131382

Purpose

Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307A in Gs MutY.

Depositor

Martin Horvath

Article

Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

Insert

EcNGsC MutY chimera F307A

Expression

Bacterial

Mutation

Fusion of two MutY genes. Residues 1-225 are from…

Promoter

tac

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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EcNGsC-S308V-pKK223

Plasmid

#131393

Purpose

Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308V in Gs MutY.

Depositor

Martin Horvath

Article

Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

Insert

EcNGsC MutY chimera S308V

Expression

Bacterial

Mutation

Fusion of two MutY genes. Residues 1-225 are fro…

Promoter

tac

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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