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GFP-LiMETER-v1

Plasmid

#113933

Purpose

Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP

Depositor

Yubin Zhou

Article

Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

Insert

LOV2 and Rit polybasic domain (PB)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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shRNA GR

Plasmid

#24095

Depositor

Moses Chao

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2008 Mar 25. 1

Insert

Glucocorticoid Receptor (Nr3c1 Rat)

Use

Lentiviral

Tags

GFP

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
pXGAD4eGFP

Plasmid

#25866

Depositor

Sergey Kasparov

Article

Wang et al FASEB J. 2006 Jul . 20(9):1537-9.

Insert

GAD4-EGFP

Expression

Mammalian

Available Since

Dec. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pGL2B -78/+64

Plasmid

#24947

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pMDC32B-AtTAS1c-D2-B/c-AtMIR173

Plasmid

#137885

Purpose

Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.

Depositor

Alberto Carbonell

Article

Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

Inserts

AtTAS1c-D2-B/c
2x35S-AtMIR173-Tnos

Expression

Plant

Mutation

A. thaliana TAS1c precursor sequence including a …

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_2xChromo

Plasmid

#179396

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_2xChromo

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

G113V in flexible linker

Promoter

CAGGS

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pNIR-FbmCherry

Plasmid

#184676

Purpose

NIR-Fb to mCherry

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-FbmCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-FAF1

Plasmid

#107986

Purpose

expression of FAF1

Depositor

Georg Weber

Article

Weber et al Sarcoma. 2015;2015:839182. doi: 10.1155/

Insert

FAF1 (FAF1 Human)

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_YTHDC1_WT

Plasmid

#81799

Purpose

Gateway Donor vector containing YTHDC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

YTHDC1 (YTHDC1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Sumire-pRsetB

Plasmid

#193361

Purpose

GFP variant emit 414 nm fluorescence.

Depositor

Takeharu Nagai

Article

Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

Insert

Sumire

Use

E.coli expression

Tags

His X6 tag

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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TaRGET-ABE-C3.1 ver4.1

Plasmid

#188251

Purpose

Hypercompact adenine base editors

Depositor

Yong-Sam Kim

Article

Kim et al Nat Chem Biol. 2022 Sep;18(9):1005-1013.

Insert

TaRGET-ABE3.1

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B1

Plasmid

#172842

Purpose

CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9037

Plasmid

#153225

Purpose

Template for PCR amplification of the constant part of guide RNAs

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

guide RNA constant part, carotenoid marker

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-aequorin

Plasmid

#101010

Purpose

Construct for expression of aequorin, calcium sensitive luciferase reporter and hygromycin resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCI 9.4

Plasmid

#74230

Purpose

Luminescent coincidence reporter with FLAG tags, FLuc-P2A-NLuc

Depositor

James Inglese

Article

Schuck et al Curr Protoc Neurosci. 2017 Apr 10;79:5.3

Insert

3xFlag-FLuc-P2A-3xFlag-NLuc

Expression

Mammalian

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MEF2C_WT

Plasmid

#81735

Purpose

Gateway Donor vector containing MEF2C , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MEF2C (MEF2C Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt8b

Plasmid

#42285

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt8b (Wnt8b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pRSLeu2d-BISopt

Plasmid

#35524

Depositor

Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

Bisabolene Synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

P GAL 1

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1228

Plasmid

#29114

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple246 (VIM Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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