We narrowed to 7,755 results for: Tet-on
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Plasmid#49525PurposeAmpicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sitesDepositorInsertP750
PromoterP750Available SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC-STITCH
Plasmid#129535PurposeSTITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.DepositorInsertSTITCH
UseCRISPR; Donor templateAvailable SinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFU-cMVIIA-PE
Plasmid#24147DepositorInsertloxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)
UseLentiviralExpressionMammalianAvailable SinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spy)
Plasmid#133316Purposeexpresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus pyogenes)
UseCRISPRPromoterxyloseAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-J23101-CocE
Plasmid#114600Purpose"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"DepositorInsertCocE
UseSynthetic Biology; Cell-free protein synthesisPromoterJ23101Available SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mTagBFP2-WPRE
Plasmid#193332PurposeExpresses mTagBFP2 under the TRE promoter in mammalian cellsDepositorInsertmTagBFP2
ExpressionMammalianMutationA XhoI site in the coding sequence is silencedPromoterTREAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5A0-T
Plasmid#92016PurposeExpresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…MutationS219VPromoterJ23102 Promoter (from Anderson promoter library) …Available SinceJan. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pyc128m
Plasmid#158778Purposesoluble GCaMP expressionDepositorInsertGCaMP6m
UseAAVAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-HA-Ago2
Plasmid#115362PurposeExpression vector to produce HA-tagged human Ago2 protein without N-peptideDepositorAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b – E. coli RecE residues 564-868
Plasmid#104534PurposeExpresses E. coli RecE residues 564-868DepositorInsertRecE residues 564-866 (C-terminal nuclease domain)
Tags6X-His tag followed by thrombin siteExpressionBacterialPromoterT7Available SinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKF-D2irTNP2AG-T2APuroR-5kwh
Plasmid#128255PurposeMammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.DepositorInserthTetR::P2A::EGFP::T2A::PuroR
UseSynthetic Biology; Flp-in expression vectorExpressionMammalianPromoterCMV-D2ir promoterAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-SAP25-126-186(pL91)
Plasmid#124387PurposeE.Coli produced SAP25 SID purified by FPLC nickel columnDepositorAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc)
Plasmid#36879DepositorInsertstTA2
tdT-3Myc
Tags3 Myc tagsExpressionMammalianMutationinsertion of a beta-globin intron with a loxP sit…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL 651
Plasmid#53016PurposeLacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).DepositorInsertsLacIq
tetR
UseSynthetic BiologyTagsRBS(st3) and T1 terminatorExpressionBacterialPromoterpConAvailable SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1277
Plasmid#53017PurposeAmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1DepositorInsertsGFP
mCherry
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialPromoterpLlacO-1 and pLtetO-1Available SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1278
Plasmid#53018PurposeAmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1DepositorInsertsmCherry
GFP
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialPromoterpLlacO-1 and pLtetO-1Available SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEST-HipO
Plasmid#114599PurposeStrong constitutive expression of metabolic enzyme, HipO, for cell-free expressionDepositorInsertHipO
UseSynthetic Biology; Cell-free protein synthesisPromoterOR2-OR1-PrAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ratFDH
Plasmid#135813PurposeExpresses rat FDH (also referred to as AldH1L1) in mammalian cells.DepositorAvailable SinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG-FLAG PLU-HIS
Plasmid#113173PurposeBaculovirus expression of PNG and PLUDepositorInsertPNG, PLU
UseBaculovirusAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHL718
Plasmid#62826PurposeLim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1DepositorInsertsgfp
mCherry
UseSynthetic BiologyTagsipex leader and ompC leaderExpressionBacterialPromoterpLlacO-1 and pLtetO-1Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only