We narrowed to 18,890 results for: Tec

1. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)

   Plasmid

   #55223

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Available Since

   July 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lentiviral-KOR-FRB

   Plasmid

   #191496

   Purpose

   Rapamycin-dependent opioid sensor for the kappa opioid receptor (component 1)

   Depositor

   Wenjing Wang

   Article

   Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

   Insert

   KOR-FRB
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-Cas9-tdTomato

   Plasmid

   #141013

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Cas9
   

   Expression

   Mammalian

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB6638

   Plasmid

   #106165

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJ915v2+Nterm Spe1 +Cterm Age1 site

   Plasmid

   #88914

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV syn CCR6-eLOV-TEVcs-tTA

   Plasmid

   #173116

   Purpose

   Expresses CCR6 receiver construct in neurons

   Depositor

   Alice Ting

   Article

   Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

   Insert

   CCR6-eLOV-TEVcs-tTA
   

   Use

   AAV

   Promoter

   Synapsin

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUCBB-ntH6-eGFP

   Plasmid

   #32557

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   eGFP (eGFP )
   

   Use

   Synthetic Biology

   Tags

   6 His tag with Thrombin site

   Expression

   Bacterial

   Promoter

   Consitutive lac

   Available Since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEU3-NII-GLICNot-C-His

   Plasmid

   #140187

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, and NotI restriction site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   6xHis and GST

   Promoter

   T7

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMB1-spect-PthrC-eGFP

   Plasmid

   #107408

   Purpose

   This plasmid contain promoter PthrC, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

   Depositor

   Kang Zhou

   Article

   Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   PthrC

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKD317.8

    Plasmid

    #181951

    Purpose

    IPTG-inducible expression of NarX

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    NarX; lacI (narX, lacI NarX - E. coli; lacI - E. coli)
    

    Expression

    Bacterial

    Promoter

    NarX-Ptac; lacI-PlacIq

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-hPDI

    Plasmid

    #60555

    Purpose

    enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

    Depositor

    Han Choe

    Article

    Song et al Appl Biochem Biotechnol. 2013 May;170(1)

    Type

    Empty backbone

    Tags

    His6-hPDI

    Expression

    Bacterial

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSplit-OC-U5-40K

    Plasmid

    #51741

    Purpose

    Express U5-40K-LucC fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    U5-40K (SNRNP40 Human)
    

    Tags

    FLAG and Luciferase (394-550aa)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pUE1-TSP-ORF68

    Plasmid

    #162650

    Purpose

    Expresses ORF68 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LLP743_L2pV1_OR_B3RT_FRT_Rluc_0I_lacZ

    Plasmid

    #192387

    Purpose

    To act as the 0-input state of the OR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFPL-Cg

    Plasmid

    #61652

    Purpose

    Gateway vector for generating cyan fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    enhanced cyan fluorescent protein
    

    Expression

    Plant

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pTS2356_Tier3(PB)-PuroR

    Plasmid

    #169655

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven PuroR expression
    

    Expression

    Mammalian

    Promoter

    PRPBSA

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTH

    Plasmid

    #56659

    Purpose

    Dual-expression vector for P. tricornutum

    Depositor

    Philip Weyman

    Article

    Weyman et al Plant Biotechnol J. 2014 Oct 10. doi: 10

    Insert

    KanR
    

    Use

    Synthetic Biology; Diatom expression

    Available Since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMM585-Tier1-PhCMV-Igk

    Plasmid

    #169486

    Purpose

    Tier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Igk secretion signal (IGK Human)
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEarleyGate103 mmPing20F

    Plasmid

    #140007

    Purpose

    mmPing20F activation tag

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    mmPing20F
    

    Expression

    Plant

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-LbCas12a

    Plasmid

    #213743

    Purpose

    For circular RNA-mediated prime editor using LbCas12a in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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