4,183 results

1. CMV-LUC2CP/intron/ARE

   Plasmid

   #62858

   Purpose

   splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

   Depositor

   Gideon Dreyfuss

   Article

   Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

   Insert

   Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   insertion of chimeric intron at nueotide poclsiti…

   Promoter

   CMV

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAS

   Plasmid

   #60847

   Purpose

   Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

   Depositor

   Jamie Cate

   Article

   Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

   Inserts

   S. pyogenese Cas9
   RNA pol III promoter (tRNA-Tyr)
   hepatitis delta virus ribozyme, genomic
   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   NLS/His8 and TTT 3' extension prior to sgRNA

   Expression

   Bacterial and Yeast

   Mutation

   L4 is UUCG tetraloop and guide targets LYP1 (CATA…

   Available Since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe puro IRES-EGFP

   Plasmid

   #14430

   Depositor

   L. Miguel Martins

   Has Service

   Cloning Grade DNA

   Insert

   IRES-EGFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-TLR3-CFP

   Plasmid

   #13641

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 3 (TLR3 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Available Since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcBac2.tR4-OMeYRS/GFP*

   Plasmid

   #50831

   Purpose

   baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

   Depositor

   Peter Schultz

   Article

   Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

   Inserts

   two-copy Tyr tRNA cassette
   EGFP*
   OMeYRS
   two copy Tyr tRNA cassette
   

   Use

   Baculoviral

   Tags

   His, Myc-6xHis, and WPRE

   Expression

   Insect

   Mutation

   Y39TAG and able to charge various unnatural amino…

   Promoter

   CAG, CMV, and U6 and H1

   Available Since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe puro HA PIK3CA H1047R

   Plasmid

   #12524

   Purpose

   Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CA H1047R (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H1047R. Tumor-derived allele.

   Available Since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-hAgo2

   Plasmid

   #21981

   Depositor

   Phil Sharp

   Article

   Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

   Insert

   Argonaute 2 (AGO2 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmTurquoise2-Mito

   Plasmid

   #36208

   Purpose

   In vivo visualization of the mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   MTS (COX8A Human)
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. PKC delta DN

   Plasmid

   #16389

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC delta isoform 2 (Prkcd Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Dominant negative (kinase inactive): K376R

   Available Since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKI1.1R

    Plasmid

    #85808

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Plant

    Promoter

    AtRPS5A

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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