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pCDNA3-HA/Bmal L115E

Plasmid

#47345

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Ha

Expression

Mammalian

Mutation

L115E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-mMcl-1 KR

Plasmid

#32981

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

All Lys to Arg

Promoter

LTR

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-h-mmPtch1-B-L235C-HA

Plasmid

#120906

Purpose

Expresses mmPtch1-B (L235C)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, L…

Promoter

CMV

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
3156 pBS Bad cDNA

Plasmid

#8752

Depositor

Stanley Korsmeyer

Article

Yang et al Cell 1995 Jan 27;80(2):285-91.

Insert

BAD (Bad Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
FUGW-H1-Syndecan-1 shRNA (UTR)

Plasmid

#40623

Depositor

Sally Temple

Article

Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

Insert

Syndecan-1 shRNA(UTR) (Sdc1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pVLAD6-mmPtch1-B-NNQ-TEV-SBP

Plasmid

#120887

Purpose

BacMam vector for Ptch1-B NNQ variant expression

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

SBP

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, N…

Promoter

CMV

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-myc- mCE(Del 25C)

Plasmid

#82472

Purpose

Expresses myc tag and mRNA capping enzyme without 25 amino acid from C-terminal

Depositor

Daniel Schoenberg

Article

Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

Insert

myc-mCE-C terminal deleted (Rngtt Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pScalps_puro_mIL-2Rα RK

Plasmid

#59920

Purpose

Expresses mouse interleukin-2 receptor alpha chain mutated

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Tags

Expression

Mutation

changed sequence of the intracellular (C-terminal…

Promoter

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
Nrp2.1-AP-His

Plasmid

#71972

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.1 (Nrp2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-mE6-AP/2525

Plasmid

#11467

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

mE6-AP (Ube3a Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

n/a

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dicer_1

Plasmid

#68807

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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PJ14

Plasmid

#25912

Depositor

Kenneth Marcu

Article

Lang et al Nucleic Acids Res. 1982 Jan 22. 10(2):61

Insert

Ig germline mu switch region

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only
pAcUW31-M10.5/human b2m

Plasmid

#11521

Depositor

Pamela Bjorkman

Article

Olson et al PLoS Biol. 2005 Aug . 3(8):e257.

Insert

M10.5 (H2-M10.4 Mouse)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-GFP

Plasmid

#75130

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pENTR-TER+-shLKB1-Ms

Plasmid

#61234

Purpose

Entry clone encoding shRNA to mouse LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (STK11, Stk11 Mouse, Human)

Use

Entr clone

Tags

Expression

Mutation

Promoter

H1/TO

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pInducer21 Flag-Sesn1b

Plasmid

#61865

Purpose

Dox inducible lentivirial expression of Flag-Sesn1b (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn1 (Sesn1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

Available since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
miR-141 reporter

Plasmid

#67632

Purpose

To quantify miR-141 function

Depositor

Judy Lieberman

Article

Le et al J Clin Invest. 2014 Dec;124(12):5109-28.

Insert

2 binding sites for miR-141

Use

Luciferase

Tags

Expression

Mutation

Promoter

SV40

Available since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
pLVTHM-shREV3-4

Plasmid

#38033

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shREV3-4 (Rev3l, Rev3l, REV3L Mouse, human, Rat, Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pMyt1(502-623)

Plasmid

#22655

Depositor

Lynn Hudson

Article

Romm et al J Neurochem. 2005 Jun . 93(6):1444-53.

Insert

Myelin Transcription Factor 1 (Myt1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

contains only first 1/2 of Myt1 central domain (C…

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only

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ATF4 19: (SL)RLuc.5’ATF4.GFP

Plasmid

#21865

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stem Loop upstream of ATF4

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-139

Plasmid

#26329

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-139 (Mir139 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hmga2 ATG wt

Plasmid

#52034

Purpose

encodes wt HMGA2

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

starting ATG deleted

Promoter

CMV

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
Mouse NCKX2-AS-long (pcDNA3.1+)

Plasmid

#75199

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Insert

SLC24A2 (Slc24a2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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mTurquoise-MyosinIIA-C-18

Plasmid

#55571

Purpose

Localization: Cytoskeleton, Excitation: 434, Emission: 474

Depositor

Michael Davidson

Insert

MyosinIIA

Use

Tags

mTurquoise

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits
3186 pBS Bcl-XL Exon 1

Plasmid

#8755

Depositor

Stanley Korsmeyer

Article

Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

Insert

Bcl-XL (Exon 1) (Bcl2l1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pENN.AAV.CB7.CI.PM20D2-flag.WPRE.rBG

Plasmid

#132684

Purpose

AAV plasmid encoding mouse PM20D2 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 2 (Pm20d2 Mouse)

Use

AAV

Tags

Flag

Expression

Mutation

Promoter

Chicken b-actin

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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mHDAC7-Unspliced (23-938) pEF6-Flag (MJS_018)

Plasmid

#73269

Purpose

mHDAC7-U (23-938) pEF6-V5 (MJS_017) (Flag tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
tdEos-Talin-IBS2-7

Plasmid

#57676

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

Talin IBS2

Use

Tags

tdEos

Expression

Mammalian

Mutation

Talin Integrin Binding Site

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCS2 YFP-Smo-Weyer's

Plasmid

#41014

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

smoothened homolog (Smo Mouse)

Use

Tags

EVC2 (aa 1177-1220) and YFP

Expression

Mammalian

Mutation

YFP inserted after Smo signal sequence. Transfer…

Promoter

Available since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV4-Flag-mIL-17RA/CFP.Hygro

Plasmid

#46861

Purpose

encodes Flag-tagged mouse IL-17RA fused to Cyan Fl. Protein

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-CFP

Use

Tags

Flag/IL-3 signal sequence and fused to CFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only