We narrowed to 22,191 results for: his

1. pBMN YFP-DHFR(DD)

   Plasmid

   #29326

   Depositor

   Thomas Wandless

   Article

   Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

   Insert

   YFP-DHFR(DD)
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…

   Promoter

   Available Since

   April 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28C-SARS-CoV-2-Nucleocapsid

   Plasmid

   #204402

   Purpose

   E. coli expression of full-length SARS-CoV-2 nucleocapsid

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-LSSmOrange-CNA35

   Plasmid

   #61605

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   LSSmOrange and CNA35
   

   Use

   Tags

   His tag

   Expression

   Mutation

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Mfn1-Myc

   Plasmid

   #23212

   Depositor

   David Chan

   Article

   Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

   Insert

   Mfn1 (Mfn1 Mouse)
   

   Use

   Tags

   6xHis, 9xMyc, and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. bdSUMO-TAT-GFP-HiBit

   Plasmid

   #170991

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-TAT-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRSFDuet-1_VNp-mNeongreen

   Plasmid

   #182387

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-His
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21b-Caspase-3

   Plasmid

   #90087

   Purpose

   Expresses wild type caspase 3 in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Wild type

   Promoter

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PET-28a-YTH domain of YTHDF2

   Plasmid

   #228776

   Purpose

   Protein expression plasmid for YTH domain of YTHDF2 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   6*His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. bdSUMO-pHD118-GFP-HiBit

   Plasmid

   #171015

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-pHD118-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. bdSUMO-pHlip-GFP-HiBit

    Plasmid

    #171016

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-pHlip-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-mAmetrine-CNA35

    Plasmid

    #61604

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mAmetrine

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mAmetrine and CNA35
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-SENP1 (catalytic domain)

    Plasmid

    #16356

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP1 catalytic domain (SENP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 419 to 644 catalytic domain of human S…

    Promoter

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQStrep2-PREI3

    Plasmid

    #31592

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    preimplantation protein 3 (MOB4 Human)
    

    Use

    Tags

    His and StrepII-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EGFPC1-hMyoX

    Plasmid

    #47608

    Depositor

    Emanuel Strehler

    Article

    Bennett et al J Biol Chem. 2007 Feb 2;282(5):3205-12.

    Insert

    MyoX (MYO10 Human)
    

    Use

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSHS207 - Bacterial expression plasmid for WT SpCas9

    Plasmid

    #101199

    Purpose

    Bacterial expression plasmid for WT SpCas9

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. eSrtA(4S-9) pET29b

    Plasmid

    #75146

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    N98D S102C A104V A118T F122A K134R F144L I182V E1…

    Promoter

    T7

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-dCas9-MQ1(WT)-EGFP

    Plasmid

    #89633

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG and 6*His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET24a-AlkB deltaN11

    Plasmid

    #73622

    Purpose

    Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

    Depositor

    Catherine Drennan

    Article

    Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    AlkB (alkB E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deletion of first 11 AA

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pAAV Syn CaMPARI2-human-a-Tubulin (TubuTag)

    Plasmid

    #164184

    Purpose

    Freeze-frame imaging of dendritic calcium signals CaMPARI2 moiety with lower Ca+ affinity (Kd=199nM) (Moeyaert et al., 2018)

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

    Inserts

    CamPARI2
    Human α Tubulin
    

    Use

    AAV

    Tags

    6 His tag and NES

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tetO-TBX5

    Plasmid

    #46032

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    TBX5 (TBX5 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available Since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pQE-80L MBP-SspB Nano

    Plasmid

    #60409

    Purpose

    E. coli expression of SspB Nano

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    SspB nano
    

    Use

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Mutation

    Y11K and A15E

    Promoter

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-SENP2 (catalytic domain)

    Plasmid

    #16357

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP2 catalytic domain (SENP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 365 to 590 catalytic domain of human S…

    Promoter

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET_RGG-RGG

    Plasmid

    #124941

    Purpose

    Expresion of RGG-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA flag PPAR gamma E499Q

    Plasmid

    #8896

    Depositor

    Bruce Spiegelman

    Article

    Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    E499Q, a mutant in the AF2 domain. Binds PPARg l…

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pQE-hTR

    Plasmid

    #21811

    Depositor

    Wafik El-Deiry

    Article

    Kim et al J Biol Chem. 2004 Sep 17. 279(38):40044-

    Insert

    TRAIL (TNFSF10 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa95∼281

    Promoter

    Available Since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

26. hHSF1 K80Q

    Plasmid

    #118351

    Purpose

    Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

    Depositor

    Lea Sistonen

    Article

    Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K80Q

    Promoter

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFDH

    Plasmid

    #131706

    Purpose

    Express constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coli

    Depositor

    Ron Milo

    Article

    Gleizer et al Cell. 2019 Nov 27;179(6):1255-1263.e12.

    Insert

    formate dehydrogenase
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mouse Drp1(K38A)

    Plasmid

    #26049

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Drp1 (Dnm1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K38A GTPase mutant

    Promoter

    Available Since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

29. pTRIP CMV VHHG4

    Plasmid

    #182236

    Purpose

    Expresses GFP nanobody for stable expression in mammalian cells

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Insert

    anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV gfaABC1D NES-jRCaMP1a

    Plasmid

    #171120

    Purpose

    Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium sensor protein.

    Depositor

    Thomas Oertner

    Article

    Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

    Insert

    jRCaMP1a
    

    Use

    AAV

    Tags

    His Tag, T7 Tag, XpressTag

    Expression

    Mammalian

    Mutation

    Promoter

    gfaABC1D

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJBL7061

    Plasmid

    #167230

    Purpose

    cell-free cascaded lead sensor

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pPbr-AKSIRV
    

    Use

    Synthetic Biology

    Tags

    6X His

    Expression

    Mutation

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-28a (+)-Gal4-vp16

    Plasmid

    #171075

    Purpose

    Expresses Gal4-vp16 fusion protein in bacteria

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4 DBD (NEWENTRY Budding Yeast)
    

    Use

    Tags

    His and VP16 AD (413-490)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA6A-EGFR ECD (1-644)

    Plasmid

    #42666

    Depositor

    Mien-Chie Hung

    Article

    Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

    Insert

    EGFR ECD (1-644) (EGFR Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3 MEKK1 KD

    Plasmid

    #12180

    Depositor

    Gary Johnson

    Article

    Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

    Insert

    MEKK1 (Map3k1 Mouse)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Kinase dead: K1253M; V1467L

    Promoter

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

35. IFI16-FL-(1-729)

    Plasmid

    #35051

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16 (IFI16 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

36. pQTEV-STMN1

    Plasmid

    #31326

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    stathmin 1/oncoprotein 18 (STMN1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET_RGG

    Plasmid

    #124929

    Purpose

    Expression of RGG domain from LAF-1 gene

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    LAF-1 RGG domain (laf-1 Nematode)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    RGG domain

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJBL7062

    Plasmid

    #167231

    Purpose

    cell-free cascaded copper sensor

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pCue-AKSIRV
    

    Use

    Synthetic Biology

    Tags

    6X His

    Expression

    Mutation

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28C-mCherry-DDX4LCD

    Plasmid

    #204408

    Purpose

    E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    DDX4(1-236) (DDX4 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET15b SARS-CoV PLpro

    Plasmid

    #61693

    Purpose

    inducible expression of SARS PLpro protease

    Depositor

    Michael Johnson

    Article

    Lee et al ChemMedChem. 2013 Aug;8(8):1361-72. doi:

    Insert

    SARS-CoV PLpro
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge