We narrowed to 34,118 results for: CMV

Showing: 4921 - 4940 of 34118 results

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U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

Plasmid

#221233

Purpose

Express sgRNA targeting HEK3 loci with nCas9(D10A)

Depositor

Fei Chen

Publication

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

SpCas9(D10A)-P2A-EGFP

Expression

Mammalian

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

Plasmid

#186980

Purpose

mammalian expression of Cluc-Gluc reporter

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

Cluc, Gluc

Use

Luciferase

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-GRAM-W

Plasmid

#211704

Purpose

Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)

Depositor

Yasunori Saheki

Publication

Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

Insert

EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Changed Glycine 187 to Tryptophan

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-GFP-TurboID-NES_pLX304

Plasmid

#202019

Purpose

expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Publication

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

Flag-GFP-TurboID-NES

Use

Lentiviral

Tags

Flag and GFP

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-FGFR1c-V5/HIS

Plasmid

#201106

Purpose

expression of human FGFR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Publication

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TRUPATH Triple Gai1

Plasmid

#196048

Purpose

Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Publication

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlphai1-RLuc8 (GNAI1 Human)
GGamma9-GFP2
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLeAPS-GFP-blast

Plasmid

#182230

Purpose

Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

Depositor

Michael Birnbaum

Publication

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

GFP-P2A-Blasticidin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmEmerald-ERGIC-53

Plasmid

#170717

Purpose

Expresses mEmerald-tagged human ERGIC-53 at high level in mammalian cells.

Depositor

Ke Xu

Publication

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

ERGIC-53 (LMAN1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSEP-LAMP1-RFP

Plasmid

#167970

Purpose

Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

Depositor

Juan Bonifacino

Publication

Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

LAMP1 (Lamp1 Rat)

Tags

SEP and mRFP

Expression

Mammalian

Mutation

D50E

Promoter

CMV

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Tubulin-mGreenLantern

Plasmid

#164460

Purpose

Localization of mGreenLantern fluorescent protein to tubulin

Depositor

Gregory Petsko

Publication

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

Tubulin

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcNDA-NES-PhoCl2c-mCherry

Plasmid

#164037

Purpose

Optogenetic control of protein localization by PhoCl2c

Depositor

Robert Campbell

Publication

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-PhoCl2c-mCherry

Tags

kozak sequence

Expression

Mammalian

Promoter

CMV promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBMNb-cALFA

Plasmid

#159991

Purpose

For the protein expression with ALFA tag at C-terminus in mammalian cells

Depositor

Motoyuki Hattori

Publication

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

HRV3C protease cleavage site-ALFA tag-twin Strep …

Expression

Mammalian

Mutation

CMV promoter, WPRE

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1

Plasmid

#155346

Purpose

Express full-length human YTHDF1 with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Publication

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (YTHDF1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHTNW

Plasmid

#136403

Purpose

Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

Depositor

Sebastian Maurer

Publication

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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mCh-KIF5B*-strep

Plasmid

#120164

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Publication

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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LAMP1-SBP-GFP

Plasmid

#120172

Purpose

Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Publication

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Lysosomal-associated membrane protein 1 (Lamp1 Rat)

Tags

SBP and eGFP

Expression

Mammalian

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEv-Fat4-citrine

Plasmid

#112854

Purpose

Constitutive expression of human fat4 protein fused to citrine fluorescent protein

Depositor

David Sprinzak

Publication

Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

Insert

FAT4 (FAT4 Human)

Tags

Citrine

Expression

Mammalian

Promoter

CMV

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCrl

Plasmid

#122443

Purpose

Expresses entire mouse reelin ORF in mammalian cells

Depositor

Tom Curran

Publication

D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

Insert

reelin (Reln Mouse)

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA_DENV2-NS2B3_V5

Plasmid

#115906

Purpose

Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag

Depositor

Alan Rothman

Publication

Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14

Insert

DENV2 NS2B3 protease

Tags

V5 tag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLNCX2-mEGFP-CHMP2B

Plasmid

#115329

Purpose

expresses mEGFP-CHMP2B in mammalian cells

Depositor

Sanford Simon

Publication

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2B (CHMP2B Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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