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pCFJ355 - MCS(ttTi14024, X)

Plasmid

#34870

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi14024 targeting

Use

Worm targeting

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pJW1310

Plasmid

#61253

Purpose

Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Publication

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

U6 promoter

Use

CRISPR; Cloning template

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAWP78-PVCpnf_pvc13-E01DARPin

Plasmid

#198287

Purpose

Produces PVCs retargeted with anti-EGFR DARPin E01

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-E01DARPin

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-forward-intron336-Venus-NLS-D2

Plasmid

#110054

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Publication

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV

Tags

NLS-D2

Expression

Mammalian

Promoter

Cry1

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-DIO-Magneto2.0-sNRPpA

Plasmid

#74307

Purpose

Cre-dependent expression of Magneto2.0 in mammalian cells

Depositor

Ali Guler

Publication

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)

Use

AAV and Cre/Lox

Tags

Flag

Expression

Mammalian

Mutation

TRPV4: delta 760-871

Promoter

CMV (after Cre recombination)

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGreen_dualluc_ORF_sensor

Plasmid

#55207

Purpose

Dual-luciferase reporter for microRNA targeting in plants

Depositor

Michael Axtell

Publication

Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

Inserts

Renilla luciferase
Firefly luciferase

Use

Luciferase; Plant transient expression

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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FUW-KM

Plasmid

#20332

Depositor

Rudolf Jaenisch

Publication

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Klf4-F2A-cMyc

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TRAF6mut-UTR

Plasmid

#15094

Depositor

David Baltimore

Publication

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRAF6 3'UTR (TRAF6 Human)

Use

Luciferase

Expression

Mammalian

Mutation

4-bp substitutions in the miR-146 target sites

Available since

June 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV-RSV-SpCas9

Plasmid

#85450

Purpose

Express SpCas9 in mammalian cells

Depositor

Hetian Lei

Publication

Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

Has service

AAV8

Insert

pRSV

Use

AAV

Promoter

pRSV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRG5271Neo

Plasmid

#26387

Depositor

Denis Dupuy

Publication

Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

Type

Empty backbone

Expression

Bacterial and Worm

Available since

Oct. 15, 2010

Availability

Academic Institutions and Nonprofits only

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1037 pGL3 FasL promoter

Plasmid

#9028

Depositor

William Sellers

Publication

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

Fas ligand promoter (FASLG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGL3-Control-PTEN-3'UTR-Mut

Plasmid

#21327

Depositor

Joshua Mendell

Publication

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTEN 3' UTR Mutant (PTEN Human)

Use

Luciferase

Expression

Mammalian

Mutation

2 miR binding site mutated. Changed from TTTGCAC …

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

Plasmid

#204146

Purpose

Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

Depositor

Jesse Bloom

Publication

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Inserts

SARS-CoV-2 BA.1 variant spike
zsGreen-T2A-PuR

Use

Lentiviral

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV and TRE3G

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAGGS-SARS2-S-G614 (C-flag)

Plasmid

#156421

Purpose

Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Publication

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Mutation

D614G

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRVL-5

Plasmid

#104583

Purpose

Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

Depositor

Daniel Christ

Publication

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Human IgG1 CH1/CH2/CH3 (IGHG1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Publication

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Expression

Worm

Promoter

rab-3

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA

Plasmid

#37090

Purpose

Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

Depositor

Bernardo Sabatini

Publication

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

hChr2(H134R)-mCherry

Use

AAV and Cre/Lox; Cre-off

Tags

mCherry

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pC0037

Plasmid

#181934

Purpose

Dual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)

Depositor

Feng Zhang

Publication

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Gaussia luciferase
Cypridina luciferase

Expression

Mammalian

Promoter

CMV and EF1alpha

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFUGW-FerH-ffLuc2-eGFP

Plasmid

#71393

Purpose

Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

Depositor

Glenn Merlino

Publication

Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

Insert

FerH-ffLuc2-eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

FerH

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-EF1a-tdTomato-WPRE-pGHpA

Plasmid

#67527

Purpose

"Expresses the fluorescent protein tdTomato"

Depositor

Botond Roska

Publication

Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

Insert

tandem dimer tomato

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available since

July 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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