We narrowed to 11,278 results for: VARS

1. pcDNA3.1-RYK-V5/HIS

   Plasmid

   #205610

   Purpose

   Expression of human RYK receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   RYK receptor tyrosine kinase (RYK Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCPH2A.X_S139D-GS3

   Plasmid

   #204748

   Purpose

   Expression of Cas9 and human H2AX with the S139D mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX S139D (H2AX Human)
   

   Expression

   Mammalian

   Mutation

   H2AX with the S139D mutation

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-AID-PcrA-UGI-T2A-mTagBFP2

   Plasmid

   #221224

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA-UGI-T2A-mTagBFP2
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. mTFP1-Caveolin-C-10

   Plasmid

   #55468

   Purpose

   Localization: Membrane, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   NM_001753 (CAV1 Human)
   

   Tags

   mTFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCK340

   Plasmid

   #192639

   Purpose

   Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dSpG
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   SoxS has R93A and S101A mutations and dSpG has se…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

   Plasmid

   #139086

   Purpose

   Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAT105

   Plasmid

   #180510

   Purpose

   Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   DpbCasX with R3 loop
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Designed VEGF-binding scFv G6des13

   Plasmid

   #110213

   Purpose

   Yeast Surface Display of G6des13

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6des13
   

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMS2214

   Plasmid

   #158417

   Purpose

   AAV plasmid with Ple265 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple265 (PCP2 Human)
   

   Use

   AAV

   Promoter

   Ple265

   Available Since

   Feb. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIYN3

    Plasmid

    #197099

    Purpose

    To express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Biochem Biophys Res Commun. 2011 Aug 12;

    Insert

    TyrRS variant, Nap3 tRNA
    

    Expression

    Bacterial

    Mutation

    Ala70, Thr158, Ser159, Arg286 (TyrRS)

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Designed VEGF-binding scFv G6des1

    Plasmid

    #110212

    Purpose

    Yeast Surface Display of G6des1

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    G6des1
    

    Tags

    cmyc tag

    Expression

    Yeast

    Mutation

    L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-ASAP2s

    Plasmid

    #101276

    Purpose

    Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

    Depositor

    Francois St-Pierre

    Article

    Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

    Insert

    ASAP2s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn (human synapsin)

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. NKP90 RFP (787)

    Plasmid

    #62001

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    NKP90 (TMEM126A Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    pgk

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pEMS2284

    Plasmid

    #158419

    Purpose

    AAV plasmid with Ple349 (PDE6H MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple349 (PDE6H Human)
    

    Use

    AAV

    Promoter

    Ple349

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pαH-S-RRAR

    Plasmid

    #164569

    Purpose

    SARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-RRAR variant)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3_GLP1R-V2R-NTEV-TCS-GV-2xHA

    Plasmid

    #194380

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
    

    Tags

    2xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHH0103_HCLS1-1/1

    Plasmid

    #91437

    Purpose

    Protein expression and purification of human SH3 domain construct HCLS1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    HCLS1-1/1 (HCLS1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiU6-hASCL1 gRNA_1-MS2-Puro

    Plasmid

    #192670

    Purpose

    Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human ASCL1 activating gRNA #1 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEMS2021

    Plasmid

    #49128

    Purpose

    AAV plasmid with DCX (Ple302) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple302-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    DCX

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pJC5-4-insulator-BREreporter-insulator

    Plasmid

    #171499

    Purpose

    injections to make Bmp reporter mouse transgenic line

    Depositor

    James Briscoe

    Article

    Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

    Insert

    Insulator BRE-MLP:H2B-Turqoise
    

    Expression

    Mammalian

    Promoter

    BRE-MLP

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only