We narrowed to 16,478 results for: por

1. pLJM60-FLAG-SLC38A9.1

   Plasmid

   #71858

   Purpose

   lentiviral stable expression

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Jan 9;347(6218):188-94. do

   Insert

   SLC38A9.1 (SLC38A9 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST47-MCU-GFP

   Plasmid

   #31732

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   *

   Promoter

   CMV

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP

   Plasmid

   #194686

   Purpose

   CMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_on-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir

   Plasmid

   #90009

   Purpose

   Split-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   FKBP-linker-Myc-NBirA*
   FRB-linker-CBirA*
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FKBP-epsin(144-575)-GFP

   Plasmid

   #100733

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   epn1 (Epn1 Mouse)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   clathrin binding middle part aa 144-575

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

   Plasmid

   #130976

   Purpose

   Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

   Insert

   Hook3-SNAPf (HOOK3 Human)
   

   Use

   Tags

   8His-ZZ-LTLT and SNAPf

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-TEK-V5/HIS

   Plasmid

   #205611

   Purpose

   Expression of human TEK receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TEK receptor tyrosine kinase (TEK Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CD8-mCherry-FRB

   Plasmid

   #100738

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CD8A (CD8A Human)
   

   Use

   Tags

   FRB and mCherry

   Expression

   Mammalian

   Mutation

   Truncated

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBa.GFP-KIF16B tail 458-1317

   Plasmid

   #134624

   Purpose

   Mammalian expression of human KIF16B 458-1317

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF16B (KIF16B Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 457 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPV-EF1a-EGxxFP(DMDex45_10)-iP-A

    Plasmid

    #204621

    Purpose

    Single-strand annealing (SSA) vector for monitoring crRNA(DMDex45)'s activity. DMD intron 44 region (upstream of exon 45)

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22b-T5-sfGFP*- MmH-MH4R

    Plasmid

    #196409

    Purpose

    Express sfGFP with TAG and enzymes for biosynthesizing DOPA

    Depositor

    Han Xiao

    Article

    Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

    Inserts

    sfGFP with TAG at 151 position
    Tyrosine Hydroxylase (Th Mouse)
    dihydropterine reductase from human (QDPR Human)
    pterin-4a-carbinolamine dehydratase from human
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2

    Plasmid

    #215326

    Purpose

    Lentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

    Depositor

    Rob Hynds

    Article

    Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

    Insert

    dNTP63 Promoter
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dNTP63

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. LENTICRISPR-UPP1_sgRNA1

    Plasmid

    #201634

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    UPP1 (UPP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDNR-SpCas9-Cdt1

    Plasmid

    #80426

    Purpose

    Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Cdt1
    

    Use

    CRISPR

    Tags

    Cdt1

    Expression

    Mutation

    codon optimized for human

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. LENTICRISPR-UPP1_sgRNA2

    Plasmid

    #201635

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    UPP1 (UPP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. A2_uSFFV

    Plasmid

    #153415

    Purpose

    Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    A*02:01-P2A-human beta2 microglobulin (B2M Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWZ403

    Plasmid

    #208113

    Purpose

    mSwAP-In marker cassette 1 with mScarlet-PuroTK

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mScarlet-PuroTK
    

    Use

    Tags

    P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla

    Plasmid

    #174620

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a cone arrestin promoter

    Depositor

    Volker Busskamp

    Article

    Gasparini et al J Clin Invest. 2022 Apr 28. pii: 154619.

    Insert

    enhanced green fluorescent protein
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. OMM-long-CFAST10

    Plasmid

    #233598

    Purpose

    Expression of CFAST10 on the OMM membrane after a long linker

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    CFAST10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRX-IP/Venus-rLC3

    Plasmid

    #58740

    Purpose

    Expresses Venus-rLC3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    Venus

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pLV-Hygro-EF1A-OB-Linker-eGFP

    Plasmid

    #176068

    Purpose

    EGFP fused to the C-terminus of an OB domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    OB
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1

    Plasmid

    #138417

    Purpose

    CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1

    Depositor

    Jian Xu

    Article

    Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

    Inserts

    BirA
    dCas9
    

    Use

    Lentiviral

    Tags

    FLAG, Avi-tag and V5, His

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PGK EGFP-PPM1H-WT

    Plasmid

    #200084

    Purpose

    Mammalian expression of wild-type PPM1H

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MLF2-GFP-3xFLAG

    Plasmid

    #230986

    Purpose

    Expresses GFP tagged MLF2 in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

    Insert

    MLF2 (MLF2 Human)
    

    Use

    Tags

    3xFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMRX-IP/SECFP-hATG16A1

    Plasmid

    #58994

    Purpose

    Expresses SECFP-hATG16A1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)
    

    Use

    Retroviral

    Tags

    SECFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-Hygro-EF1A-FHA-Linker-eGFP

    Plasmid

    #176065

    Purpose

    EGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    FHA
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40

    Plasmid

    #194690

    Purpose

    hSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40

    Plasmid

    #194691

    Purpose

    hSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_on-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hSyn-GRAB_OTmut

    Plasmid

    #185388

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Has Service

    AAV1 and AAV9

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MYH11-gRNA1

    Plasmid

    #132587

    Purpose

    gRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )

    Depositor

    James Thomson

    Article

    Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

    Insert

    MYH11-gRNA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge