We narrowed to 13,291 results for: cDNA

1. Nano-lantern-H2B/pcDNA3

   Plasmid

   #51971

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Use

   Tags

   human H2B

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3 merlin 308-590

   Plasmid

   #11627

   Depositor

   Vijaya Ramesh

   Article

   Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

   Insert

   merlin (NF2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Carboxy-terminal half of merlin isoform 2.

   Promoter

   Available Since

   May 3, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.2/V5 mmu-mir-138-1

   Plasmid

   #26328

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   mmu-mir-138-1 (Mir138-1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1-myc-AP1S3 R33W

   Plasmid

   #58294

   Purpose

   mammalian expression of AP1S3 R33W mutant

   Depositor

   Francesca Capon

   Article

   Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

   Insert

   AP1S3 (AP1S3 Human)
   

   Use

   Tags

   6xHis and myc

   Expression

   Mammalian

   Mutation

   R33W: c.97C>T,p.Arg33Trp

   Promoter

   CMV

   Available Since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-JNKsub(TA)DD-YPet-NES

   Plasmid

   #84634

   Purpose

   Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   JNKsub(TA)DD-YPet-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HA-BHLHE40(S243A/E248A)

   Plasmid

   #110155

   Purpose

   Expression of human BHLHE40 (S243A/E248A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

   Insert

   BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S243A/E248A

   Promoter

   CMV

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-4

   Plasmid

   #112839

   Purpose

   transient/stable overexpression

   Depositor

   Tomasz Prószyński

   Article

   Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

   Insert

   Grb2 (Grb2 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SH3 (N-term) domain of Grb2

   Promoter

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.2/V5 hsa-mir-483

   Plasmid

   #26315

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   hsa-mir-483 (MIR483 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-FLAG-HA-CAD S1900A

   Plasmid

   #46240

   Purpose

   FLAG-HA-CAD with S1900 mutated to alanine

   Depositor

   Brendan Manning

   Article

   Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

   Insert

   carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Serine 1900 changed to Alanine (S1900A)

   Promoter

   CMV

   Available Since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

    Plasmid

    #80272

    Purpose

    mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of multi-phospho degron (amino acids 282…

    Promoter

    CMV

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA GAL4 CREB 1-341 M1

    Plasmid

    #22973

    Depositor

    Marc Montminy

    Article

    Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

    Insert

    CREB (Creb1 GAL4-fusion, Rat)
    

    Use

    Tags

    GAL4 DNA-binding domain

    Expression

    Mammalian

    Mutation

    Changed Serine 133 to Alanine of CREB

    Promoter

    Available Since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. Nano-lantern-peroxisome/pcDNA3

    Plasmid

    #51974

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to peroxisomes

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    SKL

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA3-HA-human MYCN-V421D

    Plasmid

    #74166

    Purpose

    Mammalian expression of human MycN-VD mutant

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MycN (MYCN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    V421D

    Promoter

    CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA 3.1 SIRT1-deltaExon2

    Plasmid

    #105671

    Purpose

    Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    CMV

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1+_LN-HA-AGO2-5xE

    Plasmid

    #92011

    Purpose

    Expresses Lambda-N-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Mutation

    S824E, S828E, T830E, S831E, S834E (5xE)

    Promoter

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 Flag MFF iso4 WT

    Plasmid

    #74390

    Purpose

    Flag tagged human MFF isoform 4

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

17. 9E10.ChWT.Gal4.pCDNA1/AMP

    Plasmid

    #21914

    Depositor

    David Ron

    Article

    Wang et al Science. 1996 May 31. 272(5266):1347-9.

    Insert

    CHOP (Ddit3 Mouse)
    

    Use

    Tags

    GAL4-DNA-BD and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3-Flag-SPRTN-Y117C

    Plasmid

    #110216

    Purpose

    Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

    Depositor

    Ivana Marinovic-Terzic, Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y117C, P296L (see depositor comments below)

    Promoter

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-MIS12-LAP-BUB1

    Plasmid

    #115904

    Purpose

    Used for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.

    Depositor

    Geert Kops

    Article

    Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

    Insert

    MIS12-BUB1 (MIS12 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    BUB1 fused to MIS12

    Promoter

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.3 CAS9 IRES-Dsred

    Plasmid

    #129444

    Purpose

    expression of FnCas9 and DsRed

    Depositor

    Oskar Laur

    Insert

    FnCas9 IRES-Dsred
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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