We narrowed to 9,265 results for: yeast expression

1. pH-tdGFP

   Plasmid

   #74322

   Purpose

   pH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiae

   Depositor

   Joerg Stelling

   Article

   Roberts et al Sci Rep. 2016 Jun 21;6:28166. doi: 10.10

   Insert

   pH-tdGFP
   

   Use

   Yeast tagging

   Tags

   none

   Expression

   Yeast

   Mutation

   N149Y, Q204H

   Promoter

   Available Since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC-BETAipi-ispA/dxs

   Plasmid

   #178302

   Purpose

   Produces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Inserts

   ispA
   dxs
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS143

   Plasmid

   #122377

   Purpose

   CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PP78

   Plasmid

   #78948

   Purpose

   Supplemental Figure 3. Integration Site 5.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   Integration Site 5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

   Plasmid

   #58440

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. YIPlac204TKC-E2-Crimson-HDEL

   Plasmid

   #38771

   Depositor

   Benjamin Glick

   Article

   Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

   Insert

   E2-Crimson
   

   Use

   Tags

   Linker peptide and yeast ER retention signal sequ…

   Expression

   Yeast

   Mutation

   Promoter

   TPI1

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

   Plasmid

   #58439

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. BSP188

   Plasmid

   #110917

   Purpose

   For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

   Depositor

   Alexander Mendenhall

   Article

   Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

   Insert

   unc54 Terminator
   

   Use

   Synthetic Biology; Yeast homologous recombination…

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9v2

   Plasmid

   #103140

   Purpose

   TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

   Depositor

   Jens Nielsen

   Article

   Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

   Insert

   dCas9-VPR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Removed scaffold gRNA from original plasmid

   Promoter

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCORE-Kp53

    Plasmid

    #72241

    Purpose

    Contains the CORE cassette with KanMX4 and human p53 gene markers

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    kanMX4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V122A mutation

    Promoter

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJOG417

    Plasmid

    #105341

    Purpose

    Yeast two hybrid

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJOG418

    Plasmid

    #105342

    Purpose

    Yeast two hybrid

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDZ585 pKAN 2x-mCherry

    Plasmid

    #72236

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    2x-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

    Plasmid

    #58441

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 3 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PP74

    Plasmid

    #78944

    Purpose

    Supplemental Figure 3. Integration Site 1.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    Integration Site 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PP255

    Plasmid

    #78934

    Purpose

    Promoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS426-GFP-PLCdelta-PH

    Plasmid

    #71375

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    PLCdelta-PH
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain

    Promoter

    Available Since

    Nov. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pMAD-Gate2

    Plasmid

    #25941

    Depositor

    Kamil Onder

    Article

    Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

    Insert

    
    

    Use

    Tags

    VP16 Fusion Protein

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMOD6-pGRR-r11-r10-yeGFP

    Plasmid

    #74368

    Purpose

    pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    yeGFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGRR-r11-r10

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCORE-Hp53

    Plasmid

    #72242

    Purpose

    Contains the CORE cassette with hyg and human p53 gene markers

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    hyg
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V122A mutation

    Promoter

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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