We narrowed to 8,764 results for: ef1

1. PB-hEF1A-MG-U6-sgRNA

   Plasmid

   #175505

   Purpose

   All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1a, U6

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

   Plasmid

   #174890

   Purpose

   Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   U25C PylT

   Promoter

   EF1

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_Ef1a-->flagLaM4_Notch_tTA

   Plasmid

   #162237

   Purpose

   Lentiviral vector for the expression of LaM 4 mCherry nanobody notch tTA: flagLaM4_Notch_tTA in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   LaM4 mCherry nanobody Notch tTA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV-EF1aGFP2AKAT7-W

   Plasmid

   #159292

   Purpose

   Lentiviral vector expressing GFP and KAT7 (gRNA-resistant) driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   KAT7 (KAT7 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   CRISPR sites mutated (gRNA ID. 5 and A10)

   Promoter

   human EF1a promoter

   Available since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a_MYC deltaLZ_P2A_Hygro_Barcode

   Plasmid

   #120509

   Purpose

   Barcoded lentiviral vector to express MYC deltaLZ in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC deltaLZ (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of leucine zipper motif

   Promoter

   EF1a

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a_MYC deltaHLH_P2A_Hygro_Barcode

   Plasmid

   #120508

   Purpose

   Barcoded lentiviral vector to express MYC deltaHLH in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC deltaHLH (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of helix-loop-helix motif

   Promoter

   EF1a

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPL5606_TEF1*-Cre_ADE2

   Plasmid

   #60932

   Purpose

   Constitutive expression vector for Cre-recombinase containing ADE2 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Use

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Mutation

   Promoter

   mutant TEF1

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPL5071_TEF1*-Cre_URA3

   Plasmid

   #60930

   Purpose

   Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Use

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Mutation

   Promoter

   mutant TEF1

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1α-AausFP1-Z1

   Plasmid

   #135614

   Purpose

   AausFP1 - bright green fluorescent protein expression from A. australis under EF1a-HTLV promoter in minimal backbone plasmid.

   Depositor

   Jordan Green

   Insert

   AausFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-HTLV

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-mRor2WT

    Plasmid

    #22613

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 WT (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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