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pCDNA3 Flag MKK3b(Glu)

Plasmid

#50449

Purpose

activates p38 in the signaling pathway

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

MKK3b (MAP2K3 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 218 to Glutamic Acid, changed Thre…

Promoter

CMV

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
pMK381 (AAVS1 CMV-OsTIR1F74G)

Plasmid

#140536

Purpose

AAVS1 CMV-OsTIR1(F74G)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CMV-OsTIR1(F74G)

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q

Plasmid

#84349

Purpose

Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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GPR50-Tango

Plasmid

#66361

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR50 (GPR50 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
LentiV_Neo_SIK3_CR_T221E

Plasmid

#108108

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

change guanine 501 to cytosine (silent mutation),…

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV-MRLC1-mRuby2-IRES-PuroR

Plasmid

#103031

Purpose

Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

mRuby2

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO_HOXB13_#1

Plasmid

#70093

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
FUW TetO HA-AMPKa2 AS

Plasmid

#69827

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-37Q

Plasmid

#84351

Purpose

Expression of the human Huntingtin Exon1 protein containing 37Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pPD0055_pIVTRup_MS2-p65-HSF1

Plasmid

#242541

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

MCP-p65-HSF1 (RELA, HSF1 Human)

Use

Other

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-TBD

Plasmid

#236737

Purpose

Protein overexpression of Thalidomide Binding domain of hCRBN

Depositor

Christina Woo

Article

Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

Insert

Thalidomide Binding domain of hCRBN (CRBN Human)

Tags

His6

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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mEmerald-TGNP-N-10

Plasmid

#54279

Purpose

Localization: Golgi Complex, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

TGNP (TGOLN2 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

Plasmid

#223175

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

hU6_Rfxcas13d-DR1_BsmBI

Use

Lentiviral

Mutation

DR1 A>T

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-Golgi-7

Plasmid

#55052

Purpose

Localization: Golgi Complex, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Golgi (B4GALT1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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AAV SYN flex PSAM4 GlyR IRES EGFP

Plasmid

#119741

Purpose

Cre-dependent chemogenetic inhibitor expression

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5 and AAV9

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)

Use

AAV

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

synapsin

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_ACh3.0

Plasmid

#121922

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Has Service

AAV9

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Promoter

hSyn

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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XRE-pNL1.3[secNLuc]

Plasmid

#182295

Purpose

Secreted Luciferase reporter for AhR activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

Xenobiotic Response Element (CYP1A1 Human)

Tags

secNluc

Expression

Mammalian

Promoter

XRE

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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beta2-spectrin-HA

Plasmid

#31070

Depositor

Vann Bennett

Insert

beta2-spectrin (SPTBN1 Human)

Tags

HA

Expression

Mammalian

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TFEB-WT-MYC (#647)

Plasmid

#99955

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-GFP-Sec61β

Plasmid

#186597

Purpose

Third generation lentiviral vector expressing GFP-Sec61β

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Sec61β (SEC61B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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