We narrowed to 9,586 results for: Coli
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Plasmid#248922PurposeBacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to mCherry for nanodisc formationDepositorInsertMSP1D1_mCherry
TagsHisExpressionBacterialPromoterT7 PromoterAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1_mBlueberry2
Plasmid#248925PurposeBacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to mBlueberry2 for nanodisc formationDepositorInsertMSP1D1_mBlueberry2
TagsHisExpressionBacterialPromoterT7 PromoterAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKP2009
Plasmid#167602PurposeExpress ExbB (215-224 Ala),ExbDDepositorInsertExbB, ExbD
ExpressionBacterialMutationexbB gene with ala codons replacing codons 215-22…Available SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2010
Plasmid#167603PurposeExpress ExbB (235-244 Ala),ExbDDepositorInsertExbB, ExbD
ExpressionBacterialMutationexbB gene with ala codons replacing codons 235-24…Available SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2019
Plasmid#167604PurposeExpress ExbB (195-204 Ala),ExbDDepositorInsertExbB, ExbD
ExpressionBacterialMutationexbB gene with ala codons replacing codons 195-20…Available SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2040
Plasmid#167650PurposeExpress His6-ExbB S80A, S82A, S84ADepositorInserthis6-exbB S80A, S82A, S84A
ExpressionBacterialMutationExbB S80A, S82A, S84A with His6 tag at N terminusAvailable SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2025
Plasmid#167605PurposeExpress ExbB (225-234 Ala),ExbDDepositorInsertExbB, ExbD
ExpressionBacterialMutationexbB gene with ala codons replacing codons 225-23…Available SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2008
Plasmid#167601PurposeExpress ExbB (205-214 Ala),ExbDDepositorInsertExbB, ExbD
ExpressionBacterialMutationexbB gene with ala codons replacing codons 205-21…Available SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-TRSter
Plasmid#78865PurposeExpresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS elementDepositorInsertThioredoxin Reductase (Txnrd1 Rat)
TagsMutated E. coli-type SECIS element (3’ end of ins…ExpressionBacterialPromoterT7lacAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCH1/RAD51o
Plasmid#102562PurposeExpress Human RAD51 protein codon optimzed for E.coli and the E.coli GorE OperonDepositorInsertsHomo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
E. coli groE operon
ExpressionBacterialMutationCodon optimized for E.coli ExpressionPromoterT7Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNeae2
Plasmid#168300PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
Tags6xHis, E-tag, and myc-tagExpressionBacterialPromoterLacI-PlacAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBK-EcTrpRS(H14)-EcTyrRS(VSMA,D165G)
Plasmid#231130PurposeExpresses mutant E. coli TrpRSTyrRS from a glnS promoters for proteome-wide incorporation of tryptophan/tyrosine analogues.DepositorInsertE. coli tyrosyl tRNA synthetase
ExpressionBacterialMutationEcTrpRS: S8A, V144G, V146C; EcTyrRS: Y37V, D165G,…Available SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSEVA228-pro4IUPi
Plasmid#122018PurposeExpresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.DepositorInsertsExpressionBacterialMutationCodon optimized for E. coliPromoterpro4Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK301
Plasmid#87767PurposeE. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacIDepositorInsertPrhaBAD-sfGFP
UseSynthetic BiologyTags6xHis TagExpressionBacterialPromoterPrhaBAD rhamnose-inducible promoter from E. coliAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-EcYtR-mCherry
Plasmid#217362PurposeExpresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli tyrosine tRNA
UseAAVExpressionMammalianPromoterU6Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-MBP-4xDQNAT
Plasmid#128398PurposePeriplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationY342H- please see depositor commentsPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-EcYtR(NGS-6)-mCherry
Plasmid#217363PurposeExpresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli tyrosine tRNA mutant, "NGS-6"
UseAAVExpressionMammalianPromoterU6Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTET-IUPi
Plasmid#122019PurposeExpresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.DepositorInsertsExpressionBacterialMutationCodon optimized for E. coliPromoterpTETAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYihI-N-term D-N/E-Q
Plasmid#233095PurposeExpression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)DepositorInsertYihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
TagsGSTExpressionBacterialMutationN-term aspartic acids (D) mutated to asparagine (…Available SinceMay 7, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYihI-N-term D-A/E-L
Plasmid#233097PurposeExpression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)DepositorInsertYihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
TagsGSTExpressionBacterialMutationN-term aspartic acids (D) mutated to alanine (A) …Available SinceMay 7, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRAR051
Plasmid#185029Purpose400 nt l31 promoter region, l31 5'UTR with nt. 47-51 and 80-84 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 47-51 & 80-84 mutated rpmE 5'…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR052
Plasmid#185030Purpose400 nt l31 promoter region, l31 5'UTR with nt. 56-61 and 68-73 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 56-61 and 68-73 mutated in rpmE 5…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR104
Plasmid#185034Purpose400 nt l31 promoter region, l31 5'UTR A74 and A76 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationA74 and A76 deletion of rpmE 5'UTR, Only fir…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR105
Plasmid#185035Purpose400 nt l31 promoter region, l31 5'UTR G52 and G79 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationG52 and G79 deletion of rpmE 5'UTR, Only fir…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR107
Plasmid#185037Purpose400 nt l31 promoter region, l31 5'UTR AGA74-76GAG mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationAGA74-76GAG mutation of rpmE 5'UTR, Only fir…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR109
Plasmid#185038Purpose400 nt l31 promoter region, l31 5'UTR G60 and C69 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationG60 and C69 deletion of rpmE 5'UTR, Only fir…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR110
Plasmid#185039Purpose400 nt l31 promoter region, l31 5'UTR G62 and G67 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationG62 and G67 deletion of rpmE 5'UTR, Only fir…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR039
Plasmid#185025Purpose400 nt l31 promoter region, l31 5'UTR with nt. 1-31 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 1-31 deleted from rpmE 5'UTR, On…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR040
Plasmid#185026Purpose400 nt l31 promoter region, l31 5'UTR with nt. 35-46 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 35-46 deleted from rpmE 5'UTR, O…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR041
Plasmid#185027Purpose400 nt l31 promoter region, l31 5'UTR with nt. 47-54 & 76-86 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
ExpressionBacterialMutationNucleotides 47-54 & 76-86 deleted from rpmE …Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCapVQ329R
Plasmid#183029Purposethe patatin-like phosphodiesterase CapV variant CapVQ329R cloned in the L-arabinose inducible vector pBAD28DepositorInsertpatatin-like phospholipase variant CapVQ329R
TagsNoExpressionBacterialMutationglutamine 329 changed to argininePromoterpBAD promoterAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Ec-iPGM-bio
Plasmid#162575PurposeExpresses E. coli iPGM containing a C-terminal biotinylation site and His tagDepositorInsertE. coli iPGM bio-6His
Tags6His tag, T7 tag, biotinylation sequence, and thr…ExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP019
Plasmid#168195Purposeexpresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.DepositorInserteatA[H134R]
ExpressionBacterialMutationH134R mutationPromoteraraBADAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT1-proD-mCherry-DAS+4
Plasmid#65824PurposeConst. mCherry-DAS_4 expression , proD promoter, E. coli ExpressionDepositorInsertmCherry-DAS+4
ExpressionBacterialPromoterproD E . coli Constitutive PromoterAvailable SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEcgRNA
Plasmid#166581PurposeCRISPR-Cas9-assisted genome editing in Escherichia coliDepositorInsertccdB
ExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB15a-GFP
Plasmid#126130PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyExpressionBacterialPromoterJ23100Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB19k-GFP
Plasmid#126135PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyExpressionBacterialPromoterJ23105Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21b FAST-PETase
Plasmid#225760PurposeExpresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tagDepositorInsertFAST-PETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCB301-2μ-HDV
Plasmid#196329PurposeA yeast-Escherichia coli-Agrobacterium shuttle vectorDepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbA19k-RFP
Plasmid#126105PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterJ23105Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-pro-siRNA-V2
Plasmid#111086PurposeFor production of recombinant siRNAs (pro-siRNAs) in E. coli.DepositorTypeEmpty backboneUseRNAiExpressionBacterialAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGRB
Plasmid#71539Purposefor expression of gRNA in E. coliDepositorTypeEmpty backboneExpressionBacterialPromoterJ231196Available SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21b Hot-PETase
Plasmid#225761PurposeExpresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tagDepositorInsertHot-PETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-EFTu
Plasmid#31172DepositorAvailable SinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBB572
Plasmid#27397DepositorAvailable SinceMarch 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRM006
Plasmid#98922PurposeExpress Histag-SUMO-FtsZ for purification in E coliDepositorInsertFtsZ
TagsHistag-SUMOExpressionBacterialPromoterT7Available SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSU-araC-Cas9
Plasmid#120423PurposeMultigene editing in the Escherichia coli genome using the CRISPR-Cas9 systemDepositorInsertCas9
UseCRISPRExpressionBacterialPromoteraraBADAvailable SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJAT13araE
Plasmid#18987DepositorAvailable SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
EcRho
Plasmid#113121PurposeExpresses E. coli RhoDepositorInsertRho
TagsMGHExpressionBacterialPromoterT7Available SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-eGFP-MinD
Plasmid#133622PurposeExpression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.DepositorAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only