We narrowed to 1,640 results for: plasmid for e coli

1. pSCL.72

   Plasmid

   #184984

   Purpose

   Express Cas9/-Eco1 RT fusion, using linker1

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Cas9-fusion_linker1-Eco1RT
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSCL.95

   Plasmid

   #184986

   Purpose

   Express Cas9/-Eco1 RT fusion, using linker2

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Cas9-fusion_linker2-Eco1RT
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSCL.94

   Plasmid

   #184985

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker2

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker2-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSCL.037

   Plasmid

   #184966

   Purpose

   Express -Eco1 dead RT and ncRNA in yeasst

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Human codon optimized RT, YXDD catalytic region m…

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSCL.71

   Plasmid

   #184983

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker1

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker1-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSCL.112

   Plasmid

   #184981

   Purpose

   Express -Eco1 FAA1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   FAA1 donor P233stop

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSCL.002

   Plasmid

   #184972

   Purpose

   Express -Eco1 ADE2 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ADE2 donor P272stop

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSCL.110

   Plasmid

   #184979

   Purpose

   Express -Eco1 TRP2 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   TRP2 donor E64stop

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQE31-6xHis-Countdown

   Plasmid

   #182299

   Purpose

   E. coli plasmid encoding Countdown with a hexahistidine tag

   Depositor

   Andrew York

   Article

   Seidel et al (2021), doi: 10.5281/zenodo.5810930

   Insert

   Countdown
   

   Use

   Tags

   histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPP085

    Plasmid

    #158795

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-6His-MBP-MpAgo

    Plasmid

    #79249

    Purpose

    This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

    Depositor

    Jennifer Doudna

    Article

    Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

    Insert

    Marinitoga piezophila Argonaute
    

    Use

    CRISPR

    Tags

    6XHis-tag + MBP + PreScission Cleavage Site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPP089

    Plasmid

    #158796

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPP537

    Plasmid

    #158803

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Use

    Tags

    2xSV40NLS-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPP076

    Plasmid

    #158794

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPP378

    Plasmid

    #158797

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    dCasPhi-1 (D371A)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPP384

    Plasmid

    #158799

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    dCasPhi-3 (D413A)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHBT-Mb(GDx-Clo_L10)

    Plasmid

    #183406

    Purpose

    Expresses Monobody GDx-Clo_L10 in E. coli

    Depositor

    Shohei Koide

    Article

    Kermani et al Nat Commun. 2020 Nov 27;11(1):6064. doi:

    Insert

    His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)
    

    Use

    Tags

    His tag-Avi tag-TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXD69m1-TetW

    Plasmid

    #191246

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lenta

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEA02

    Plasmid

    #172193

    Purpose

    Suicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.

    Depositor

    Jean-Luc Pernodet

    Article

    Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

    Insert

    attL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZA16mflon

    Plasmid

    #75439

    Purpose

    Plasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    mf-lon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. I766T USP7 UBL-12

    Plasmid

    #227232

    Purpose

    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    I766T; aa 535-775

    Promoter

    T7-promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. L757P USP7 UBL-12

    Plasmid

    #227231

    Purpose

    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L757P; aa 535-775

    Promoter

    T7-promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. R152C USP7 TRAF

    Plasmid

    #227229

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    R152C; aa 62-205

    Promoter

    T7-promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. G392A FL USP7

    Plasmid

    #227217

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G392A

    Promoter

    T7-promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. L469R USP7 catalytic

    Plasmid

    #227212

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L469R; aa 207-560

    Promoter

    T7-promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. N418S FL USP7

    Plasmid

    #227215

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    N418S

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. H111Q USP7 TRAF

    Plasmid

    #227227

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    H111Q; aa 62-205

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. G392D FL USP7

    Plasmid

    #227216

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G392D

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. K420E FL USP7

    Plasmid

    #227223

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    K420E

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. L299A USP7 catalytic

    Plasmid

    #227213

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L299A; aa 207-560

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. V485G USP7 catalytic

    Plasmid

    #227209

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    V485G; aa 207-560

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. P273H USP7 catalytic

    Plasmid

    #227208

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    P273H; aa 207-560

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Y331H USP7 catalytic

    Plasmid

    #227205

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Y331H; aa 207-560

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. M225I USP7 catalytic

    Plasmid

    #227206

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    M225I; aa 207-560

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. USP7 N418S catalytic

    Plasmid

    #227202

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    N418Sl; aa 207-560

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. L373F FL USP7

    Plasmid

    #227220

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L373F

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Y331H FL USP7

    Plasmid

    #227218

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Y331H

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. P273H FL USP7

    Plasmid

    #227221

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    P273H

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. M225I FL USP7

    Plasmid

    #227219

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    M225I

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. K420E USP7 catalytic

    Plasmid

    #227210

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    K420E; aa 207-560

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. USP7 G392D catalytic

    Plasmid

    #227203

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G392D; aa 207-560

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. F118L USP7 TRAF

    Plasmid

    #227228

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    F118L; aa 62-205

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. L469R FL USP7

    Plasmid

    #227225

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L469R

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. E345K USP7 catalytic

    Plasmid

    #227211

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    E345K; aa 207-560

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. L373F USP7 catalytic

    Plasmid

    #227207

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L373F; aa 207-560

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. G392A USP7 catalytic

    Plasmid

    #227204

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G392A; aa 207-560

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. E345K FL USP7

    Plasmid

    #227224

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    E345K

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. V485G FL USP7

    Plasmid

    #227222

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    V485G

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pOK12

    Plasmid

    #49789

    Purpose

    E. coli cloning vector (KanR, low copy, blue/white selection)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pQE31-6xHis-pHCountdown

    Plasmid

    #182300

    Purpose

    E. coli plasmid encoding pH-Countdown with a hexahistidine tag

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    pH Countdown
    

    Use

    Tags

    histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge