We narrowed to 7,101 results for: tev

1. GFP-BICD2N

   Plasmid

   #64070

   Purpose

   N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)

   Depositor

   Andrew Carter

   Article

   Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

   Insert

   BICD2N (1-400) (Bicd2 Mouse)
   

   Use

   Tags

   GFP and His-ZZ-TCS

   Expression

   Insect

   Mutation

   Codon optimized for Sf9 expression

   Promoter

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A

   Plasmid

   #127790

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in His2…

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pfastbacHTb/mClock26-384

   Plasmid

   #47336

   Purpose

   mClock fragment cloned into pfastbacHTb vector with Hexa His tag

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   His and Tev

   Expression

   Mutation

   aa 26-384

   Promoter

   PH

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pY71-tet.I.8

   Plasmid

   #133521

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Use

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. hnRNPA2_190-303

   Plasmid

   #118822

   Purpose

   Bacterial expression of hnRNPA2 truncation (residues 190 to 302).

   Depositor

   Nicolas Fawzi

   Article

   Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   His tag and TEV

   Expression

   Bacterial

   Mutation

   contains amino acids 190-302

   Promoter

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. V32M,L84M CoV-2 ORF8

   Plasmid

   #159934

   Purpose

   Protein overexpression in E.coli

   Depositor

   James Hurley

   Article

   Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

   Insert

   CoV-2 ORF8 18-121 (ORF8 CoV-2)
   

   Use

   Tags

   6x Histidine, TEV cleavable

   Expression

   Bacterial

   Mutation

   V32M, L84M

   Promoter

   T7

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST

   Plasmid

   #175018

   Purpose

   Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

   Insert

   OK-HEF s57a ED
   

   Use

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   s57a esterase knock out mutant

   Promoter

   CMV

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCD5-ss-D/bovine/France/5920/2014-HEFwtED-GCN4-sfGFP-ST

   Plasmid

   #175017

   Purpose

   Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

   Insert

   OK-HEFwtED
   

   Use

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEXP5-NT-CuSeCat

   Plasmid

   #42568

   Depositor

   Ivan Korendovych

   Article

   Mack et al J Biol Inorg Chem. 2013 Apr;18(4):411-8.

   Insert

   mutated calmodulin gene
   

   Use

   Tags

   Polyhistidine (6×His) region, TEV recognition sit…

   Expression

   Bacterial

   Mutation

   changed Phenylalanine 92 to Glutamine acid, Valin…

   Promoter

   T7

   Available since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pY71-tet.I.16

    Plasmid

    #133536

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET151-Vps4(101-437)-Hcp1 K321A/R325A

    Plasmid

    #89287

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Lys-321 and Ar…

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLAP-MIS12-KARD

    Plasmid

    #114057

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    MIS12-KARD (BUB1B Human)
    

    Use

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

    Plasmid

    #101213

    Purpose

    Bacterial expression plasmid for SpCas9 + K855A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K855A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K855A

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBiex PKC - Peptide 14 mCit mCer

    Plasmid

    #83565

    Purpose

    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

    Insert

    PKC alpha (PRKCA Human)
    

    Use

    Tags

    10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…

    Expression

    Bacterial and Insect

    Mutation

    Only catalytic domain of PKC (aa 335-672)

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac-hDcr-K70A.

    Plasmid

    #89145

    Purpose

    Expression of helicase mutant of human Dicer in Sf9 cells

    Depositor

    Jennifer Doudna

    Article

    MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

    Insert

    hDicer (DICER1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Insect

    Mutation

    K70A

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX4T-1_OCT4_T234E_S235E

    Plasmid

    #40635

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV Cleavage, and Thrombin Cleavage

    Expression

    Bacterial

    Mutation

    T234E_S235E

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pGEX4T-1_OCT4_T234E

    Plasmid

    #40636

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV Cleavage, and Thrombin Cleavage

    Expression

    Bacterial

    Mutation

    T234E

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pGEX4T-1_OCT4_T234A

    Plasmid

    #40637

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV cleavage, and Thrombin cleavage

    Expression

    Bacterial

    Mutation

    T234A

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pGEX4T-1_OCT4_S235E

    Plasmid

    #40638

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV Cleavage, and Thrombin Cleavage

    Expression

    Bacterial

    Mutation

    S235E

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pFastBac1-ScVip1-186-1107

    Plasmid

    #238316

    Purpose

    ScVip1-186-1107 protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-186-1107

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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