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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,320 results for: Set

Showing: 7531 - 7560 of 8320 results

  2. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

    Plasmid
    #157984
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    157984-plasmid-map-sequence-id-355759

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  3. Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

    Plasmid
    #157985
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    157985-plasmid-map-sequence-id-332449

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  6. Th-iCre

    Plasmid
    #159459
    Purpose
    Targeting vector to TH gene locus for induction of P2A-fused iCre, with PGK-puroR drug resistant cassette flanked by FRT sequence.
    Depositor
    Yuejun Chen
    Article
    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-
    Insert
    P2A-iCre
    Use
    Cre/Lox
    Tags
    P2A
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous TH
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    159459-plasmid-map-sequence-id-319220

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  7. pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184739
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184739-plasmid-map-sequence-id-361530

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  10. pENTR-AtTAS1c-D2-B/c

    Plasmid
    #137883
    Purpose
    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]
    Depositor
    Alberto Carbonell
    Article
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Insert
    AtTAS1c-D2-B/c
    Use
    Gateway-compatible entry clone
    Tags
    Expression
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137883-plasmid-map-sequence-id-275808

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  13. pAC8_MF-pH-Flag-Cter (VE5739)

    Plasmid
    #161799
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal Flag tag
    Use
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    161799-plasmid-map-sequence-id-324153

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  14. pAC8_MF-PH-HA-Nter-P10-mCherry (VE5740)

    Plasmid
    #161800
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a N-ter HA tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    N-terminal HA tag
    Use
    Tags
    HA
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    161800-plasmid-map-sequence-id-324127

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  15. pAC8_MF-PH-Flag-Nter (VE5627)

    Plasmid
    #139773
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an N-ter Flag tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    N-terminal Flag tag
    Use
    Tags
    Flag
    Expression
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139773-plasmid-map-sequence-id-324119

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  16. pAC8_MF-PH-HA-Cter (VE5629)

    Plasmid
    #139775
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter HA tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal Hemaglutinine tag
    Use
    Tags
    HA
    Expression
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139775-plasmid-map-sequence-id-324156

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  17. pAC8_MF-PH-6His-Cter (VE5630)

    Plasmid
    #139777
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter6His tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal 6His tag
    Use
    Tags
    6 His
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139777-plasmid-map-sequence-id-324174

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  18. pAC8_MF-PH-c-Myc-Cter (VE5632)

    Plasmid
    #139778
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter C-Myc tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal C-Myc tag
    Use
    Tags
    c-Myc
    Expression
    Insect
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139778-plasmid-map-sequence-id-324155

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  19. pAC8_MF-PH-3C-10His-Cter (VE5622)

    Plasmid
    #139771
    Purpose
    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter 10-His tag under the pH promoter.
    Depositor
    Arnaud Poterszman
    Article
    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.
    Insert
    C-terminal 10-His tag
    Use
    Tags
    3C - 10His
    Expression
    Mutation
    Promoter
    PH
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    139771-plasmid-map-sequence-id-324118

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  20. pEcRNAP5

    Plasmid
    #128939
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoAppx234-241-(His)10 (rpoA Escherichia coli)
    RpoB
    RpoC
    RpoZ
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Residues 234-241 are substituted for a PreScissio…
    Promoter
    T7
    Available since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    128939-plasmid-map-sequence-id-254599

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  21. pLC-Flag-UBE2G1 sg2R-P2A-Hygro

    Plasmid
    #124299
    Purpose
    Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.
    Depositor
    Eva Gottwein
    Article
    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.
    Insert
    UBE2G1 (UBE2G1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    several silent mutations to disrupt sgRNA targeti…
    Promoter
    CMV
    Available since
    July 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124299-plasmid-map-sequence-id-244604

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  23. pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184740
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184740-plasmid-map-sequence-id-361528

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  25. pBB0323-TEV-His12

    Plasmid
    #137039
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137039-plasmid-map-sequence-id-291948

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  28. FB003

    Plasmid
    #119677
    Purpose
    Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119677-plasmid-map-sequence-id-238439

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  30. pAM4663

    Plasmid
    #85126
    Purpose
    NSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.
    Depositor
    Susan Golden
    Article
    Chang et al Science. 2015 Jul 17;349(6245):324-8. do
    Insert
    PkaiB-luciferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85126-plasmid-map-sequence-id-180127

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Showing: 7531 - 7560 of 8320 results