We narrowed to 8,587 results for: reporter

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PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

Plasmid

#102888

Purpose

PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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p221z-erTagRFP

Plasmid

#71266

Purpose

Entry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

TagRFP

Use

Gateway

Tags

ER retention signal HDEL and signal peptide (ER)…

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFPV25-cR-2iG

Plasmid

#204618

Purpose

Fluorescent reporter for SPI2 induction in Salmonella

Depositor

Jacques Neefjes

Article

Stévenin et al Cell Rep. 2024 Nov 1;43(11):114931. doi:

Inserts

rpsM promoter
sseA promoter

Tags

GFP and dsRed

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSyn1-EGFP

Plasmid

#153187

Purpose

Mouse synapsin-1 (mSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pME V5-mTagBFP2 (JDW 830)

Plasmid

#224484

Purpose

Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mTagBFP2

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template

Plasmid

#167943

Purpose

SFTPC reporter targeting repair template

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

SFTPC (SFTPC Human)

Use

CRISPR; Donor template

Tags

Venus

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

EF1a

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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p221k-erTq2CFP

Plasmid

#71264

Purpose

Entry clone containing ER-localized Turqoise2. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system.

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

Tq2CFP

Use

Gateway

Tags

ER retention signal HDEL and signal peptide (ER)…

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUBC-EBFP2

Plasmid

#212314

Purpose

Lentiviral backbone with EBFP2 reporter

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV intron Renilla STOP 12xPP7 TNFα-ARE xrRNA12 24xMS2 SV40 CTE polyA

Plasmid

#104096

Purpose

Expresses TREAT-ARE reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Tags

12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

Expression

Mammalian

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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3xnls-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129626

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-cpmTq2-Calcium-lifetime-sensor

Tags

3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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ST-SBP-mCherry_puromycin

Plasmid

#65271

Purpose

ST reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAG27

Plasmid

#226742

Purpose

Expresses a reporter for a mitochondrial matrix protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

COX4 (COX4I1 Human)

Use

Lentiviral

Tags

GFP1-10

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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CD 44 pGL4basic

Plasmid

#78135

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

CD 44 (CD44 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

CD44

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSHUT4-eYFP

Plasmid

#133094

Purpose

Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI

Depositor

Jens Laurids Sørensen

Article

Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25

Insert

enhanced yellow flourescent protein

Use

Fungal expression

Promoter

TEF-1α

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2143

Plasmid

#104356

Purpose

AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-smCBA-EmGFP-WPRE

Use

AAV

Promoter

smCBA

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCMS-dsRed2-C1824U LMNA-GFP minigene

Plasmid

#128231

Purpose

C1824U mutation Lamin A splicing reporter

Depositor

Tom Misteli

Article

Shilo et al Nucleic Acids Res. 2019 Apr 22. pii: 547

Insert

lamin A (LMNA Human)

Tags

GFP and SV40-driven dsRed2 to identify transfecte…

Expression

Mammalian

Mutation

C1824U

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-hLMX1A-T2A-copGFP

Plasmid

#170444

Purpose

Lentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.

Depositor

Sang-Hun Lee

Article

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

human LMX1A

Use

Lentiviral

Promoter

EF1a

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMIR-CDH1 wtUTR

Plasmid

#25038

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

CDH1 3'UTR (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pGL3-BASIC-ARHGAP29 PROMOTER

Plasmid

#104155

Purpose

luciferase reporter with ARHGAP29 promoter

Depositor

Marius Sudol

Article

Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

Insert

ARHGAP29 promoter (ARHGAP29 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

ARHGAP29

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-EcYtR(NGS-6)-mCherry

Plasmid

#217363

Purpose

Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

E. coli tyrosine tRNA mutant, "NGS-6"

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-PytR(PyOtR)-mCherry

Plasmid

#204870

Purpose

Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

M. mazei pyrrolysyl tRNA mutant "PyOtR"

Use

AAV

Expression

Mammalian

Mutation

U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

Promoter

U6

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

Plasmid

#179437

Purpose

Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

coreHS4-TRE-cit-coreHS4-mch (DC)

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-B-161;+80pCalb2

Plasmid

#66745

Purpose

luciferase reporter for Calb2 promoter (-161to +80, WT)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/mCherry-DMDEx23

Plasmid

#211367

Purpose

Expresses a DMD exon 23 skipping reporter in mammalian cells (mCherry-DMDEx23)

Depositor

Ulrich Lächelt

Article

Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

Insert

mCherry-DMDEx23

Tags

eGFP

Expression

Mammalian

Mutation

mCherry interrupted by mdx dystrophin exon 23 bet…

Promoter

CMV

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDN-T2dMFot

Plasmid

#44558

Depositor

Gabor Balazsi

Article

Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

Inserts

PTETREG promoter
rtetR-M2::FFF

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

Promoter

PTETREG

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-D2irTN6kwh

Plasmid

#44724

Depositor

Gabor Balazsi

Article

Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

Inserts

pCMV-D2i promoter
htetR::NLS

Use

Synthetic Biology; Expression regulator/reporter;…

Tags

Rabbit β-globin intron II and WPRE

Expression

Mammalian

Mutation

Initiator motif (Inr) displaced relative to pCMV-…

Promoter

pCMV-D2i

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pMIR-9

Plasmid

#25037

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

miR-9 binding site (MIR9-1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pGL3-B-264;+80pCalb2

Plasmid

#66746

Purpose

luciferase reporter for Calb2 promoter (-264 to +80, WT)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-264bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-B-419;+80pCalb2

Plasmid

#66747

Purpose

luciferase reporter for Calb2 promoter (-419 to +80, WT)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-419bp+80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Calb2

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-Tet1 promoter A

Plasmid

#63881

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 promoter fragment A

Use

Luciferase

Expression

Mammalian

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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