We narrowed to 13,664 results for: sequence
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Plasmid#102355PurposePlasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning siteDepositorInsertTAL-L1-1.3
UseT3 in vitro transcription for expression in mamma…TagsFLAG and HAAvailable SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pV1F
Plasmid#205084PurposeProkaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.DepositorInsertAtVIP1
ExpressionBacterialPromoteragorbacterium virB operon promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-rac2 guide
Plasmid#107600PurposeAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expressionDepositorInsertrac2 guide RNA sequences
UseCRISPRAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
V1-MESA-45F-M-tTA
Plasmid#84500PurposeMESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and tTADepositorInsertV1-MESA-45F-M-tTA
UseAAVTagsFlagExpressionMammalianPromoterCMVAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB131
Plasmid#68585PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsEmerald Luc fused with PEST sequence [ELuc(PEST)]ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNP1 sfGFP trigger
Plasmid#107359PurposeTrigger sequence for pNP1 sfGFP toehold switchDepositorInserttoehold trigger 7
ExpressionBacterialPromoterT7Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_NES_C1
Plasmid#85062PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
ExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-1.3-VP64
Plasmid#103033PurposePlasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activatorDepositorInsertTAL-L1-1.3-VP64
UseT3 in vitro transcription for expression in mamma…TagsHA and VP64Available SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
SBP-EGFP-Ecadherin_puromycin
Plasmid#65292PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
TagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AktAR-Kras
Plasmid#123357PurposeFRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.DepositorInsertAktAR-Kras
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-2.4-VP64
Plasmid#103039PurposePlasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activatorDepositorInsertTAL-L1-2.4-VP64
UseT3 in vitro transcription for expression in mamma…TagsHA and VP64Available SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-Cas9)
Plasmid#194768PurposeTo insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.DepositorInsertT2A-Cas9
UseCRISPRExpressionInsectAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
JDW 1050 (pME_V5_GATA1_P2A_H2A_mCherry)
Plasmid#232127PurposeA middle entry gateway clone containing V5 tagged human GATA1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt
Plasmid#194519PurposeDual fluorescence reporter for studying protein degradationDepositorInsertTIF4631 leader sequence
ExpressionYeastAvailable SinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPTI-[5,55]Gly14Ile38
Plasmid#195059PurposeThermal stability of BPTIDepositorInsertbovine pancreatic trypsin inhibitor
TagsHis tag and trpΔLE leader sequencesExpressionBacterialMutationChanged Alanine residues at position of 14 and 38…PromoterT7 promotorAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-1.3-KRAB
Plasmid#103034Purpose[Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by KRAB domainDepositorInsertTAL-L1-1.3-KRAB
UseT3 in vitro transcription for expression in mamma…TagsHA and KRABAvailable SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
N-terminal Halo-XLF
Plasmid#207546PurposeHomologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human Lig4 locus sequences
TagsHaloTagExpressionMammalianAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB331
Plasmid#68659PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsEmerald Luc fused with PEST sequence [ELuc(PEST)]ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pShoHELIX-sgRNA_entry (BO3)
Plasmid#181784PurposeExpresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-miR9/9*
Plasmid#177805PurposeThe pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.DepositorInsertmiR-9/9*
ExpressionMammalianAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SUMO FAAA)3
Plasmid#130703PurposeMammalian expression plasmid for negative control of mCherry-(SUMO)3.DepositorInsert3 copies of human SUMO1 separated by (GGS)4
ExpressionMammalianMutationFKVK sequence in each SUMO1 module changed to FAAAPromoterCMVAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_human-4xUAS
Plasmid#125150PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSA120
Plasmid#59789PurposeC. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operonDepositorInsertshsp-16.41 promoter
zif-1 coding
gpd-2/gpd-3 intergenic sequence
mCherry (worm)
unc-54 3' UTR
ExpressionBacterialAvailable SinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
SEPTOPO
Plasmid#64944Purposesource of SEP (Super Ecliptic pHluorin) sequenceDepositorInsertSEP
ExpressionBacterialPromoterlacAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-μDpTT
Plasmid#182321PurposePlasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertLyn-μDronpa-TagRFP-T
TagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LaG9(S151C)
Plasmid#172490PurposeEncodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).DepositorInsertLaG9(S151C)
Tags6xHis, PPX cleavage site, and pelB leader sequenc…ExpressionBacterialMutationS151CPromoterT7Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-2.4-Ctrl
Plasmid#103037PurposePlasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning siteDepositorInsertTAL-L1-2.4
UseT3 in vitro transcription for expression in mamma…TagsFLAG and HAAvailable SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-miR124
Plasmid#177806PurposeThe pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.DepositorInsertmiR-124
ExpressionMammalianAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
eTC GFP ß-actin ΔZip
Plasmid#27124DepositorInsertbeta-actin (ACTB Human)
TagsEGFP and TC tagExpressionMammalianMutationThis construct lacks β-actin 3′UTR and therefore …Available SinceJan. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-'S-mito
Plasmid#158765PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
ExpressionMammalianMutationFlexible linkers between the protease and msGFP a…Available SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only