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pPB-EF1a-MegaGate-DD-Hygro

Plasmid

#175267

Purpose

MegaGate destination with C-terminal DD tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

Destabilization Domain

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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M-ST1-sgRNA

Plasmid

#48672

Purpose

Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter

Use

CRISPR

Promoter

hU

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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M-NMcas

Plasmid

#48670

Purpose

Mammalian N. meningitidis Cas9 expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9

Use

CRISPR

Tags

NLS

Promoter

CMV

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only
betaRBPIG

Plasmid

#16422

Depositor

Juergen Hescheler

Article

Pfannkuche et al Nat Protoc. 2008. 3(9):1510-26. doi: htt

Insert

loxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP

Expression

Mammalian

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pLEX_307_ms2_VPR

Plasmid

#79376

Purpose

Recruits VPR to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-VPR

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDD379

Plasmid

#91834

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Type

Empty backbone

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_p65_hsf1

Plasmid

#79374

Purpose

Recruits p65_hsf1 to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-p65-hsf1

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJKR-O-acuR

Plasmid

#62569

Purpose

Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

CFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCZGY2727

Plasmid

#193330

Purpose

Site specific insertion using CRISPR/Cas9 editing of C. elegans ChrI

Depositor

Yishi Jin

Article

Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

Insert

Hygromycin resistance

Expression

Worm

Promoter

Prps-0

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD16

Plasmid

#208268

Purpose

Production of syn-stemar-13-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL8

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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M-tdTom-SP

Plasmid

#48677

Purpose

Mammalian tdTomato activation reporter for SP with GTCCCCTCCACCCCACAGTG protospacer

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, SP-PAM, and tdTomato reporter. Compatible with S. pyogenes Cas9

Use

CRISPR

Promoter

Sp6

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only
M-SPcas

Plasmid

#48668

Purpose

Mammalian S. pyogenes Cas9 expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9

Use

CRISPR

Tags

NLS

Promoter

CMV

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9C-P2A-turboGFP

Plasmid

#80935

Purpose

Plasmid that expresses Cas9C in mammalian cells; co-expresses turboGFP.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-FAF1S181G

Plasmid

#107987

Purpose

expression of oncogenic FAF1

Depositor

Georg Weber

Article

Weber et al Sarcoma. 2015;2015:839182. doi: 10.1155/

Insert

FAF1S181G (FAF1 Human)

Expression

Mammalian

Mutation

carcinogenic mutant, S181G

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pZA72

Plasmid

#174783

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_XopJ4, Kmr plant selection marker

Expression

Plant

Mutation

WT + STOP codon

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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dSp-VP64-p65-RTA(75-190)

Plasmid

#99670

Purpose

Expresses dSp Cas9 fused to combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

VP64-p65-RTA(75-190)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET23a-Mm/HsUBA52

Plasmid

#69560

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBA52 (UBA52 Human)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
M-tdTom-NM

Plasmid

#48679

Purpose

Mammalian tdTomato activation reporter for NM with GTCCCCTCCACCCCACAGTG protospacer

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, NM-PAM, and tdTomato reporter. Compatible with N. meningitidis Cas9

Use

CRISPR

Promoter

Sp6

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only
pSY45

Plasmid

#48355

Purpose

Targets TbMCM-BP for Cre-lox mediated knockout in T. brucei

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

loxP-SAS-HYG-Ty1-TK-loxP flanked by TbMCM-BP-targeting homologies

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-hdNadV

Plasmid

#83362

Purpose

Expresses nadV from Haemophilus ducreyi in Escherichia coli

Depositor

George Cătălin Marinescu

Article

Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

Insert

nadV

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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